| ABC Day Care & Preschool | 2855 Lake Helen - O'steen Road, Deltona, FL 32738 | Preschool |
| Amanda's Childcare & Preschool | 123 W Rhode Island Rd, Orange City, FL 32763 | Preschool |
| Amanda's Childcare II | 115 W. Rhode Island Road, Orange City, FL 32763 | Center |
| Blake Memorial Dayschool | 134 N Euclid Avenue, Lake Helen, FL 32744 | Center |
| Boston Avenue Preschool | 340 N. Boston Avenue, Deland, FL 32724 | Preschool |
| Casa Montessori | 217 North Stone Street, Deland, FL 32720 | Preschool |
| Child's World of Learning | 2700 Enterprise Road, Orange City, FL 32763 | Preschool |
| Children First Academy | 825 S Spring Garden Avenue, Deland, FL 32720 | Preschool |
| Children's House Montessori School | 509 E Pennsylvania Avenue, Deland, FL 32724 | Center |
| Children's Playskool | 1748 South Woodland Blvd., Deland, FL 32720 | Preschool |
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| Coleman Head Start Center | 200 East Blue Springs, Orange City, FL 32763 | Preschool |
| Cornerstone Christian Learning Center | 2813 Howland Blvd, Deltona, FL 32725 | Center |
| DeLand Family YMCA Childcare | 761 E Int'l Speedway Blvd, Deland, FL 32724 | Preschool |
| Deland High School Infant - Toddler Development Center | 800 North Hill Avenue, Deland, FL 32724 | Center |
| Easter Seals of Volusia & Flagler County | 2219 S Woodland Blvd, Deland, FL 32720 | Preschool |
| Emmaus Lutheran Preschool | 2500 S Volusia Ave, Orange City, FL 32763 | Preschool |
| First Baptist Early Childhood Development Center | 725 N Woodland Blvd, Deland, FL 32720 | Preschool |
| First Baptist Preschool Learning Center | 975 E Graves Avenue, Orange City, FL 32763 | Preschool |
| First Presbyterian Church Day School | 724 North Woodland Boulevard, Deland, FL 32720 | Preschool |
| First United Methodist Church of DeLand - Preschool | 115 East Howry Avenue, Deland, FL 32724 | Preschool |
| Forever Learning Center | 227 S. Florida Ave., Deland, FL 32720 | Center |
| Gingerbread Childcare | 138 E French Avenue, Orange City, FL 32763 | Preschool |
| Great Beginnings Learning Center | 337 Old Daytona Road., Deland, FL 32724 | Preschool |
| Johnson Academy of Early Learning | 465 S. Volusia Avenue, Orange City, FL 32763 | Preschool |
| Kid's Korner Development Center | 1400 N Clara Avenue, Deland, FL 32720 | Preschool |
| Kiddie Koledge | 1114 W. Beresford Avenue, Deland, FL 32720 | Preschool |
| Kids Life! | 346 N. Volusia Avenue, Orange City, FL 32763 | Center |
| Kidz Connection Learning Center | 1785 Elkcam Blvd, Deltona, FL 32725 | Center |
| Kidz Learn & Play | 541 S Volusia Avenue, Orange City, FL 32763 | Center |
| King's Kids Academy | 3185 Yorkshire Drive, Deltona, FL 32738 | Center |
| Learning Bridge Academy | 2411 E. Graves Avenue, Orange City, FL 32763 | Center |
| Lighthouse Christian Academy - DeLand | 126 S Ridgewood Avenue, Deland, FL 32720 | Center |
| Lil Lambs Christian Pre School | 401 E. Taylor Road, Deland, FL 32724 | Center |
| Little Cherubs Learning Academy | 107 S Chandler Avenue, Deland, FL 32724 | Preschool |
| Malloy Head Start Center | 330 E Retta St, De Leon Springs, FL 32130 | Preschool |
| Mater Christi Preschool PSG | 50 Mansfield Avenue, West Greenwich, RI 02817 | Center |
| Merry Day Child Care Center | 407 E Kentucky Avenue, Deland, FL 32724 | Preschool |
| My Little Friends Preschool | 285 W Blue Springs Ave, Orange City, FL 32763 | Preschool |
| New Creation Academy | 1250 S. Spring Garden Avenue ..., Deland, FL 32720 | Preschool |
| New Horizons Babies & Toddlers | 227 S Florida Ave, Deland, FL 32720 | Center |
| New Horizons Discovery Centers | 500 S Dexter Avenue, Deland, FL 32720 | Preschool |
| Rainbow Child Care Center | 1401 W New York Avenue, Deland, FL 32720 | Preschool |
| Saint Barnabas Episcopal School | 322 West Michigan Avenue, Deland, FL 32720 | Preschool |
| St. Barnabus Episcopal School | 322 West Michigan Avenue, Deland, FL 32720 | Preschool |
| Stepping Stones Child Care | 1575 Detrick Avenue, Deland, FL 32724 | Preschool |
| Stetson Baptist Christian School | 1025 W Minnesota Avenue, Deland, FL 32720 | Preschool |
| The Little House Next Door Child Development | 133 E. Church Street, Deland, FL 32720 | Preschool |
| Trinity Christian Academy Preschool | 875 Elkcam Boulevard, Deltona, FL 32725 | Preschool |
| Trinity Learning Center | 306 W Wisconsin Ave, Deland, FL 32720 | Preschool |
| Twinkle Star Center | 238 S Amelia Ave, Deland, FL 32724 | Preschool |
| Twinkle Toes Early Learning Center | 399 N Orange Avenue, Orange City, FL 32763 | Preschool |
| West Volusia Educational Center | 803 South Woodland Boulevard, Deland, FL 32720 | Preschool |