| Central Street Connection | 301 E. Central Street, Trenton, TN 38382 | Center |
| Charlotte's Day Care | 120 Johnson Street, Trenton, TN 38382 | Center |
| Children At Play Learning Center | 1709 Stallings Road, Humboldt, TN 38343 | Center |
| Christian Learning Center | 2000 Jones Blvd., Milan, TN 38358 | Preschool |
| Emma's Enrichment Center | 7005 Graball Road, Milan, TN 38358 | Center |
| First Baptist Children's Learning Center | 3400 Mitchell St., Humboldt, TN 38343 | Preschool |
| Gadsden Elementary Leaps | 18989 Hwy 79, Gadsden, TN 38337 | Center |
| Gadsden Elementary Pre-kindergarten | 18989 Hwy 79, Gadsden, TN 38337 | Preschool |
| Hannah's And Doris' Prayer | 910 N. Central Street, Humboldt, TN 38343 | Center |
| Hannah's Prayer Day Care | 704 Elliott St., Humboldt, TN 38343 | Center |
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| Humboldt City Preschool | 301 Westside Drive, Humboldt, TN 38343 | Preschool |
| Humboldt Head Start | 152 Humboldt-gibson Hwy., Humboldt, TN 38343 | Preschool |
| Johnson's Baby World | 408 E. Second Street, Trenton, TN 38382 | Center |
| Kid's Land Child Care Center | 612 N. Central Avenue., Humboldt, TN 38343 | Center |
| Kinder Academy Learning Center | 7005 Graball Drive, Milan, TN 38358 | Center |
| Lemac - Trenton Housing Authority | 435 East Second Street, Trenton, TN 38382 | Center |
| Little People's Day Care | 413 E. Fourth St., Trenton, TN 38382 | Center |
| Little People's Jump Start | 409 E. 4th St., Trenton, TN 38382 | Center |
| M.C.L.C. | 375 T. West Church Street, Medina, TN 38355 | Center |
| Medina Elementary Pre-kindergarten | 227 North Second Street, Medina, TN 38355 | Preschool |
| Medina School Age Child Care Extended School Program | 227 North Second Street, Medina, TN 38355 | Center |
| Paula's Playground | 57 Concord-moore's Chapel, Trenton, TN 38382 | Center |
| Pollard's Day Care | 502 So. Brownsville St., Trenton, TN 38382 | Center |
| Presbyterian Day School | 1528 Presbyterian Drive, Humboldt, TN 38343 | Center |
| Professional Child Care II | 1740 Industrial Drive, Trenton, TN 38382 | Center |
| Sherry's Day Care | 52 State Route 186 South, Humboldt, TN 38343 | Center |
| Small Wonders | 621 Gibson Road, Trenton, TN 38382 | Center |
| T & G Miracle Day Care # I | 1015 N. 17th Avenue, Humboldt, TN 38343 | Center |
| T & G Miracle Day Care II | 400 South 17th Avenue, Humboldt, TN 38343 | Center |
| T L C Day Care | 77 Dyersburg Hwy., Trenton, TN 38382 | Center |
| Tennessee State University TN Cares Early Head Start | 421 E. 2nd Street, Trenton, TN 38382 | Preschool |
| Tennessee State University TN Cares Early Head Start | 21 Hadley Drive, Humboldt, TN 38343 | Preschool |
| Tiny Tots Daycare | 980 Old Hwy 70e, Trenton, TN 38382 | Center |
| Trenton Elementary School Extended School Program | 811 South College Street, Trenton, TN 38382 | Center |
| Trenton Pre-kindergarten Early Childhood Program | 811 South College St., Trenton, TN 38382 | Preschool |
| Trenton SSD -Head Start Pre-kindergarten | 811 South College, Trenton, TN 38382 | Preschool |