| Administrative Service Center | 500 S Spaulding, Pueblo, CO 81007 | Center |
| Ascension Preschool | 420 W 18th ST, Pueblo, CO 81003 | Preschool |
| Belmont Preschool | 31 MacNaughton RD, Pueblo, CO 81001 | Preschool |
| Bessemer Preschool | 1125 E Routte, Pueblo, CO 81003 | Preschool |
| Bradford Preschool | 107 S LaCrosse, Pueblo, CO 81001 | Preschool |
| Building Blocks | 34 Montebello RD, Pueblo, CO 81001 | Center |
| Central Christian Preschool | 1902 N Hudson, Pueblo, CO 81001 | Preschool |
| Child's Play Day Care | 807 San Angelo, Pueblo, CO 81001 | Center |
| Desert Sage Elementary | 935 S Palomar DR, Pueblo, CO 81007 | Center |
| East Side Child Care Center | 2717 E 8th ST, Pueblo, CO 81001 | Preschool |
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| Ecems at CSU - Pueblo Child Care Center | 2200 Bonforte BLVD, Pueblo, CO 81001 | Preschool |
| Eva Baca Preschool | 2800 E 17th ST, Pueblo, CO 81001 | Preschool |
| Franklin Preschool | 1315 Horseshoe DR, Pueblo, CO 81001 | Preschool |
| Goodnight Preschool | 624 Windy WAY, Pueblo, CO 81005 | Preschool |
| Haaff Preschool | 15 Chinook LN, Pueblo, CO 81001 | Preschool |
| Highland Park Preschool | 2701 Vinewood LN, Pueblo, CO 81005 | Preschool |
| Imagination Station | 2102 S Prairie BLVD, Pueblo, CO 81005 | Center |
| Irving Preschool | 1629 W 21st ST, Pueblo, CO 81003 | Preschool |
| John Neumann Preschool | 2415 E Orman, Pueblo, CO 81004 | Preschool |
| Mcclelland School | 415 E Abriendo, Pueblo, CO 81004 | Preschool |
| Minnequa Medicenter Daycare | 2701 California ST, Pueblo, CO 81004 | Center |
| Nature & Raptor Center of Pueblo | 5200 Nature Center RD, Pueblo, CO 81003 | Center |
| North Mesa Elementary | 28881 Gale RD, Pueblo, CO 81006 | Preschool |
| Parkview Preschool #2 | 1327 E 9th ST, Pueblo, CO 81001 | Preschool |
| Pueblo School District No. 70 | 24951 E Highway 50, Pueblo, CO 81006 | Center |
| Shrine of St Therese Catholic School | 320 Goodnight AVE, Pueblo, CO 81004 | Preschool |
| Somerlid Preschool | 2717 West ST, Pueblo, CO 81003 | Preschool |
| Southside Building Blocks Learning Center | 95 Lehigh, Pueblo, CO 81005 | Center |
| Southside Childrens Center | 2601 Sprague, Pueblo, CO 81004 | Center |
| Spann Preschool | 2300 E 10th ST, Pueblo, CO 81001 | Preschool |
| Thatcher Learning Center at Parkview | 522 W 15th ST, Pueblo, CO 81003 | Center |
| The Children's Palace | 973 E Spaulding AVE, Pueblo, CO 81007 | Preschool |
| The Mcclelland School Preschool | 415 E Abriendo, Pueblo, CO 81004 | Preschool |
| Washington Childrens Center | 401 S Prairie AVE, Pueblo, CO 81005 | Center |
| Youth and Family Academy Infant Nursery | 1920 Valley DR, Pueblo, CO 81008 | Center |