| Lisa's Lots Of Love Day Care | 2103 Knickerbocker Ave., Medford, NY 11763 | Preschool |
| Appleton Preschool Early Learning Center and Child Care | 397 Appleton St, Holtsville, NY 00544 | Preschool |
| Bay Area Head Start | 161 Margin Drive, Shirley, NY 11967 | Preschool |
| Bellport Area Community Action Committee | 37 Kreamer Street, Bellport, NY 11713 | Center |
| Bellport Area Community Action Committee | 599 Brookhaven Avenue, Bellport, NY 11713 | Center |
| Boys & Girls Club of the Bellport Area | 471 Atlantic Avenue, Bellport, NY 11713 | Center |
| Bright Horizons at Holtsville | 1 Motorola Plaza, Holtsville, NY 11742 | Center |
| Brookhaven Country Preschool | 79 Long Island Avenue, Yaphank, NY 11980 | Preschool |
| Brookhaven National Laboratory Child Development Center | 373 Yale Road, Upton, NY 11973 | Center |
| Care Center Day Care | 247 Cabot St, Holtsville, NY 00544 | Center |
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| Castle Kids | 1401 Montauk Highway, Mastic, NY 11950 | Center |
| Center for Youth and Family Services | 368 Maple St, Holtsville, NY 00544 | Center |
| Central Brookhaven Head Start | 2884 Route 112, Medford, NY 11763 | Preschool |
| Churchill Head Start | 41 Commercial St, Holtsville, NY 00544 | Center |
| Colonial Youth and Family Services | 201 Montauk Highway, Moriches, NY 11955 | Center |
| Colonial Youth and Family Services | 2 Van Buren Street, Mastic, NY 11950 | Center |
| Cornerstone Kiddie Kollege | 576 Granny Road, Medford, NY 11763 | Center |
| Developmental Disabilities Institute-Medford Campus | 1 Scouting Blvd, Medford, NY 11763 | Center |
| East End Kids Academy | 177 Weeks Avenue, Manorville, NY 11949 | Center |
| Girls Incorporated of Holyoke | 52 Nick Cosmos Way, Holtsville, NY 00544 | Center |
| Holyoke Boys and Girls Club | 70 Nick Cosmos Way, Holtsville, NY 00544 | Preschool |
| Imagine BSC Kids | 2 Corporate Drive, Holtsville, NY 11742 | Preschool |
| Imagine Learning Center | 94b Montauk Highway, East Moriches, NY 11940 | Preschool |
| J. C. Learning Center | 40 Brown Ave, Holtsville, NY 00544 | Preschool |
| J.C. Learning Center | 251 Appleton St, Holtsville, NY 00544 | Preschool |
| Julie's Story Book Nursery | 145b Montauk Highway, Westhampton, NY 11977 | Center |
| Just Kids Early Childhood Learning Center | 35 Longwood Rd, Middle Island, NY 11953 | Preschool |
| Just Kids at William Floyd | 99 Lexington Rd., Shirley, NY 11967 | Center |
| Kiddie Kampus | 3235 Route 112, Medford, NY 11763 | Center |
| Kiddie Kollege | 135 Clark St., Patchogue, NY 11772 | Center |
| Kids' World Nursery School | 21 Center Court, Center Moriches, NY 11934 | Center |
| KinderCare at Farmingville - CLOSED | 2270 North Ocean Ave., Farmingville, NY 11738 | Preschool |
| L.I.H.S.C.D.S. Bellport Head Start | 189 N. Dunton Ave, Patchogue, NY 11772 | Preschool |
| Life Line Mediation Center | 41 Yaphank-middle Island Road, Middle Island, NY 11953 | Center |
| Little People's Child Care Center | 208 Jamaica Ave, Medford, NY 11763 | Center |
| Long Island Head Start - Patchogue | 20 Church Street, Patchogue, NY 11772 | Preschool |
| Long Island School of the Arts | 6 Francis Landau Place, Shirley, NY 11967 | Center |
| Master Heo's Tae Kwon Do After School Martial Arts Program | 225 South St, Holtsville, NY 00544 | Center |
| Middle Island Caring for Kids | 629 Middle Country Road, Middle Island, NY 11953 | Center |
| Patchogue Early Head Start | 10 Church Street, Patchogue, NY 11772 | Preschool |
| Reach For the Stars Day Care | 1720 Middle Country Rd, Ridge, NY 11961 | Center |
| Red Robin East Country Day School | 184 Main Street, Center Moriches, NY 11934 | Center |
| Rubber Ducky Child Care #3 | 1723 North Ocean Avenue, Medford, NY 11763 | Center |
| SCOPE at Bayport/Blue Point Elementary | 212 Blue Point Ave., Blue Point, NY 11715 | Center |
| SCOPE at East Moriches | 523 Montauk Hwy., East Moriches, NY 11940 | Center |
| SCOPE at Ridge | 105 Ridge Rd., Ridge, NY 11961 | Center |
| SNAP Long Island | 630 Moriches-middle Island Road, Moriches, NY 11955 | Center |
| St. Paul's Nursery School | 485 Appleton St, Holtsville, NY 00544 | Preschool |
| Starbright Children's Center | 40 North Ocean Avenue, Center Moriches, NY 11934 | Center |
| Summerfield Child Care Center | 74 Summerfield Drive Suite B, Holtsville, NY 11742 | Center |
| The Kids' Place | 303 Homestead Ave, Holtsville, NY 00544 | Preschool |
| The Learning Experience | 2920 Route 112, Medford, NY 11763 | Center |
| The New Interdisciplinary School | 430 Sills Rd., Yaphank, NY 11980 | Center |
| The Patchogue YMCA | 255 West Main Street, Patchogue, NY 11772 | Center |
| Tutor Time Child Care Middle Island | 1225 Middle Country Rd-strathmore Apt. Commons, Middle Island, NY 11953 | Center |
| Tutor Time Learning Center - East Patchogue | 655 E. Main St., Patchogue, NY 11772 | Preschool |
| Tutor Time of Medford | 703 Patchogue Yaphank Road, Medford, NY 11763 | Center |