| Alpha and Omega Child Care II | 429 Main St, Pevely, MO 63070 | Center |
| Angels In Joyland | 450 Bailey Rd, Crystal City, MO 63019 | Preschool |
| Bright Beginning Learning Center | 1549 W Main St, Festus, MO 63028 | Center |
| Children of Destiny Child Care | 15533 Gamel Cemetery Rd, Festus, MO 63028 | Center |
| Daytime Discoveries | Po Box 275, Valmeyer, IL 62295 | Center |
| For Kids Only Ltd | 5432 B Highway 61 67, Imperial, MO 63052 | Center |
| Immanuel Lutheran Christian Childcare and Preschool | 19 N 3rd St, Festus, MO 63028 | Preschool |
| Immanuel Lutheran Parish Pre School | 110 Hoener Ave, Waterloo, IL 62298 | Preschool |
| Jefferson R -7 Preschool | 2400 R-7 School Rd, Festus, MO 63028 | Preschool |
| Jefferson R7 Kids Korner | 2400 R-7 School Rd, Festus, MO 63028 | Center |
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| Kid's Academy of Waterloo | 409 Lakeview Dr, Waterloo, IL 62298 | Center |
| Little Scholars Preschool | 409 Deer Crossing Dr, Festus, MO 63028 | Preschool |
| Lori Collop Dulaney | 109 Williamsburg Dr, Crystal City, MO 63019 | Center |
| Mustard Seed Preschool | 6439 Highway 61-67, Imperial, MO 63052 | Preschool |
| Osceola Migrant Head Start Center | Hwy 61 North, Osceola, AR 72370 | Center |
| Prime Time Child Care Academy | 5181 Warren Rd, Imperial, MO 63052 | Preschool |
| Sacred Heart's Ursuline Learning Center | 201 Brierton Ln, Festus, MO 63028 | Center |
| Shannon Cancienne | 5512 Lehigh Ln, Imperial, MO 63052 | Center |
| St Lukes Preschool | 1101 Sulphur Springs Rd, Ballwin, MO 63021 | Preschool |
| The Lighthouse Learning Center | 261 Covington, Waterloo, IL 62298 | Center |
| The Lighthouse Learning Center | 303 Covington, Waterloo, IL 62298 | Center |
| The Playground Child Care Center | 1302 Kenner St, Crystal City, MO 63019 | Center |
| Valley View Day Care and Learning Center | 2010 Seckman Rd, Imperial, MO 63052 | Preschool |
| Valmeyer Elementary School Preschool | 300 S Cedar Bluff Dr, Valmeyer, IL 62295 | Preschool |
| Wee Care Lc | 821 American Legion Dr, Festus, MO 63028 | Preschool |
| Westbrook Academy Childcare and Learning Center | 4045 Jeffco Blvd, Arnold, MO 63010 | Preschool |
| YMCA Jefferson County Branch Festus | 1500 Mid Meadows Dr, Festus, MO 63028 | Center |
| YMCA Jefferson County Branch Our Ladys | 1550 St Mary's Ln, Festus, MO 63028 | Center |
| YMCA Jefferson County Crystal City Elementary | 600 Mississippi, Crystal City, MO 63019 | Center |
| YMCA of Jefferson County Dunklin R-v | 30 Main St, Pevely, MO 63070 | Center |
| YMCA of Jefferson County Lil Rascals Preschool | 1303 Ymca Dr, Festus, MO 63028 | Preschool |