| Gingerbread House Learning Center | 6005 Chester Ave., Jacksonville, FL 32217 | Preschool |
| Grace Lutheran School | 12200 Mccormick Road, Jacksonville, FL 32225 | Preschool |
| Happy Acres Ranch | 7117 Crane Avenue, Jacksonville, FL 32216 | Preschool |
| Harvest Christian Academy | 1057 Arlington Road, Jacksonville, FL 32211 | Center |
| Head Start at Anders Park | 10770 Anders Blvd #407, Jacksonville, FL 32246 | Preschool |
| Head Start at Fairfield | 515 Victoria Street, Jacksonville, FL 32202 | Preschool |
| Head Start at Ft. Caroline Road | 5543 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Head Start at Jacksonville Urban League 8th St | 20 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Head Start at Lee Road | 1210 Lee Road, Jacksonville, FL 32225 | Preschool |
| Head Start at Northeast Springfield | 1221 E 16th Street, Jacksonville, FL 32206 | Preschool |
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| Head Start at Old Stanton | 521 West Ashley Street, Jacksonville, FL 32202 | Preschool |
| Head Start at Pearson | 1314 West Union Street, Jacksonville, FL 32209 | Preschool |
| Head Start at Robert F Kennedy | 1133 Ionia St, Jacksonville, FL 32206 | Preschool |
| Head Start at The Bridge | 320 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Headstart at Shands | 2052 Jefferson Street, Jacksonville, FL 32209 | Preschool |
| Headstart at Southwind Villas | 8711 Newton Road, Jacksonville, FL 32216 | Preschool |
| Hendricks Day School | 1824 Dean Road, Jacksonville, FL 32216 | Preschool |
| Hidden Hills Learning Tree | 12160 Ft. Caroline Rd., Jacksonville, FL 32225 | Preschool |
| Hidden Treasures Child Care Center | 1941 Dean Road, Jacksonville, FL 32216 | Preschool |
| Highlands Precious Gifts Preschool | 10900 Mccormick Road, Jacksonville, FL 32225 | Preschool |
| Hodges Boulevard Presbyterian Church | 4140 Hodges Boulevard, Jacksonville, FL 32224 | Preschool |
| Hogan Christian Preschool | 8045 Hogan Road, Jacksonville, FL 32216 | Preschool |
| Holy Family | 9800-3 Baymeadows Road, Jacksonville, FL 32256 | Preschool |
| Holy Spirit Catholic School | 11665 Fort Caroline Road, Jacksonville, FL 32225 | Preschool |
| Honey Child Pre-School | 2627 Spring Glen Road, Jacksonville, FL 32207 | Center |
| Hope Haven Children's Clinic and Family Center | 4600 Beach Blvd, Jacksonville, FL 32207 | Preschool |
| Hopes-n-Dreams Child Care | 6689 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Intercoastal Kids | 13109 Professional Drive, Jacksonville, FL 32225 | Preschool |
| Isle of Faith Child Development Center | 1821 San Pablo Road, Jacksonville, FL 32224 | Preschool |
| Ivy League Academy at San Pablo | 3232 San Pablo Road South, Jacksonville, FL 32224 | Preschool |
| Jacksonville Adventist Academy | 4298 Livingston Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Jewish Center | 3662 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Urban League Head Start | 3811 St Augustine Road, Jacksonville, FL 32207 | Preschool |
| Jewish Community Alliance | 8505 San Jose Boulevard, Jacksonville, FL 32217 | Center |
| John Love Elementary Preschool | 1531 Winthrop Street, Jacksonville, FL 32206 | Preschool |
| Joyful Sounds Christian Academy | 10750 Atlantic Boulevard Suite 11, Jacksonville, FL 32225 | Preschool |
| Just 4 Kids Learning Academy | 4371 Losco Rd., Jacksonville, FL 32257 | Preschool |
| Justina Road Elementary Preschool | 3101 Justina Road, Jacksonville, FL 32277 | Preschool |
| Kats Little Kittens Learning Center | 3692 Beach Blvd., Jacksonville, FL 32207 | Preschool |
| Kid Kampus U S A | 1868 Parental Home Road, Jacksonville, FL 32216 | Preschool |
| Kid'z on Top II Child Care & Learning Center | 5571 Playa Way, Jacksonville, FL 32211 | Preschool |
| Kids By The Hour | 13947 Beach Boulevard Suite 210, Jacksonville, FL 32224 | Preschool |
| Kids By The Hour II | 2771 Monument Rd #3, Jacksonville, FL 32225 | Preschool |
| Kids Expanding the Kingdom Christian Preschool | 3749 Crownpoint Road, Jacksonville, FL 32257 | Preschool |
| Kids Konnection | 2221 Noble Street, Jacksonville, AL 36265 | Center |
| Kids United Childcare & Learning Center | 7037-1 Phillips Highway Suite 1, Jacksonville, FL 32216 | Preschool |
| Kids World of Florida | 6150 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Kids World of Florida | 2597 Oak Street, Jacksonville, FL 32204 | Preschool |
| Kids in Dynamic Settings | 3271 Tiger Hole Road, Jacksonville, FL 32216 | Center |
| Kidz Quest Academy | 1820 University Boulevard North, Jacksonville, FL 32211 | Preschool |
| Kidz n Kidz Academy | 6229 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Kimbari Learning Academy | 3651 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Kinder Kollege Learning Center | 1403 Myrtle Avenue North, Jacksonville, FL 32209 | Center |
| Kingdom Kids at CrossRoad | 10005 Gate Parkway North, Jacksonville, FL 32246 | Preschool |
| Kings and Queens Child Care and Learning Center | 3325 Plateau Street, Jacksonville, FL 32206 | Center |
| La Core Christian Academy | 122 Arlington Road, Jacksonville, FL 32211 | Preschool |
| La Escuelita Pre School | 5415 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Lakewood Presbyterian Church Preschool | 2001 University Boulevard West, Jacksonville, FL 32217 | Preschool |
| Lakewood United Methodist Preschool | 6133 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
| Learning Ladders Child Development Center | 11270 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| Leslie's Angels | 2114 Tilden Street, Jacksonville, FL 32206 | Center |
| Lighthouse Christian Preschool | 2115 12th Avenue North, Jacksonville Beach, FL 32250 | Preschool |
| Lil' Sprouts Learning Center | 8840-7 Atlantic Blvd., Jacksonville, FL 32211 | Preschool |
| Little Britches | 10829 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Little Diamonds Big Heart Day Care | 1441 East 31st Street, Jacksonville, FL 32206 | Center |
| Little Friends | 4001 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Little Saints Child Care Center | 2179 Emerson Street, Jacksonville, FL 32207 | Preschool |
| Little Sarephims Daycare | 220 Mill Creek Road, Jacksonville, FL 32211 | Center |
| Little Stars Preschool | 545 A1a North, Ponte Vedra Beach, FL 32082 | Preschool |
| Little Temples Child Care Center II | 9100 Merrill Road Unit 15, Jacksonville, FL 32225 | Preschool |
| Little Wise Kids Early Learning Center | 6318 Arlington Road, Jacksonville, FL 32211 | Center |
| Little Wise Kids Early Learning Center II | 5703 Arlington Road, Jacksonville, FL 32211 | Center |
| Lone Star Learning Center | 8142 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Long Branch Elementary Preschool | 3723 Franklin Street, Jacksonville, FL 32206 | Preschool |
| Magellan Academy - CLOSED | 14985 Old St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| Magellan Christian Academies | 10550 - 704 Deerwood Park Boul ..., Jacksonville, FL 32256 | Preschool |
| Mandarin Childhood Learning Center | 4228 Hood Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Prep School | 3968 Sunbeam Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Presbyterian Preschool | 11844 Mandarin Rd., Jacksonville, FL 32223 | Preschool |
| McCrary's Learning Center | 6620 Arlington Expressway, Jacksonville, FL 32211 | Preschool |
| Mine Yours & Ours Child Care Learning Center | 7645 Merrill Road #107, Jacksonville, FL 32277 | Preschool |
| Montessori Tides School | 1550 Penman Road, Jacksonville Beach, FL 32250 | Preschool |
| Monument Christian Academy | 1509 Mayport Road, Atlantic Beach, FL 32233 | Center |
| Monument KinderCare - CLOSED | 12040 McCormick Rd, Jacksonville, FL 32225 | Preschool |
| Naval Air Station Jacksonville Child Development Center | 705 Child Street, Jacksonville, FL 32212 | Preschool |
| Naval Station Mayport Child Development Center | 1137 Cove Landing Drive, Atlantic Beach, FL 32233 | Preschool |
| Neptune Baptist W.E.E. School | 407 3rd Street, Neptune Beach, FL 32266 | Preschool |
| New Dimensions Learning Center | 3612 Belfort Road, Jacksonville, FL 32216 | Preschool |
| Northern Lights Sunshine Academy | 6240 Fort Caroline Road #1-4, Jacksonville, FL 32277 | Preschool |
| Once Upon A Time Development Center | 1129 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Open Arms Christian Childcare Center | 9039 Beach Boulevard, Jacksonville, FL 32216 | Preschool |
| Page of Learning | 5809 Southpoint Parkway, Jacksonville, FL 32216 | Preschool |
| Palms Preschool & Extended Day Program | 3410 3rd Street South, Jacksonville Beach, FL 32250 | Preschool |
| Parkwood Weekday Early Education Center | 7900 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Parson's Little Scholars Creative Child Care | 6505 Fort Caroline Road, Jacksonville, FL 32277 | Preschool |
| Parsons Christian Academy | 5705 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Peas In A Pod Learning Center | 9652 Hood Road, Jacksonville, FL 32257 | Preschool |
| Pokey's Angelic Christian Children's Academy | 3863 St. Augustine Road, Jacksonville, FL 32207 | Preschool |
| Pooh + Friends Child Care Development Center | 6029 Bennett Road, Jacksonville, FL 32216 | Preschool |
| Power Challengers Ministries | 4316 Barnes Road, Jacksonville, FL 32207 | Center |