| Ankeny KinderCare | 3055 N. Ankeny Blvd, Ankeny, IA 50021 | Center |
| Childtime of Des Moines | 610 Center St, Des Moines, IA 50309 | Center |
| East Ridge KinderCare | 4025 Benjamin Dr, Saint Paul, MN 55125 | Center |
| Grimes KinderCare | 1101 NE Gateway Dr., Grimes, IA 50111 | Center |
| Johnston KinderCare | 8601 Thomas Ave, Johnston, IA 50131 | Center |
| La Petite Academy of Ankeny | 929 East 1St St, Ankeny, IA 50021 | Center |
| Oralabor KinderCare | 225 SW Oralabor Road, Ankeny, IA 50023 | Center |
| Pleasant Hill KinderCare | 1425 Copper Creek Dr, Des Moines, IA 50327 | Preschool |
| 2 For You Day Care | 6950 NE 14th St Stes 36-38, Ankeny, IA 50023 | Center |
| Adventure Life Preschool | 1700 8th St SW, Altoona, IA 50009 | Center |
|
| Altoona 8th Street Child Care | 907 8Th St SW, Altoona, IA 50009 | Center |
| Altoona Campus Kids Klub - Centennial Site | 910 7th Ave SE, Altoona, IA 50009 | Center |
| Altoona Campus Kids Klub - Clay Elementary | 3200 1St Ave S, Altoona, IA 50009 | Center |
| Altoona Campus Kids Klub - Delaware Elementary | 4401 NE 46th St, Des Moines, IA 50317 | Center |
| Altoona Campus Kids Klub - Willowbrook | 300 17th Ave SW, Altoona, IA 50009 | Center |
| Altoona Kidsworld Daycare - Preschool #2 | 630 8th St SE, Altoona, IA 50009 | Center |
| America's Children of Oakmoor | 4731 Merle Hay Rd, Urbandale, IA 50322 | Center |
| Angeli Cristiani Childcare | 2200 Beaver Ave, Des Moines, IA 50310 | Center |
| Ankeny Christian Academy Childcare | 1604 W 1st St, Ankeny, IA 50023 | Center |
| Ankeny Christian Childcare | 2506 SW 3rd St, Ankeny, IA 50023 | Center |
| Apple Tree Children's Center - JOH | 5623 NW 86th St Suite 100, Johnston, IA 50131 | Center |
| Apple Tree Childrens Center - ANK | 225 E 1st St, Ankeny, IA 50021 | Center |
| Bear Basics Children's Center | 135 Douglas Ave, Des Moines, IA 50313 | Center |
| Beautiful Beginnings Preschool & Childcare Center | 1701 W Jester Park Dr, Polk City, IA 50226 | Center |
| Bethany Preschool | 720 NE 52nd Ave, Des Moines, IA 50313 | Center |
| Bob and Billie Ray Child Development Center | 401 NE 66th Ave, Des Moines, IA 50313 | Center |
| Boulevard Children's Center | 900 Polk Blvd, Des Moines, IA 50312 | Center |
| Bright Beginnings Preschool at A First United Methodist Church | 206 SW Walnut St, Ankeny, IA 50023 | Center |
| Bright Horizons At The Downtown School | 1800 Grand Ave, Des Moines, IA 50309 | Center |
| CFI Child Development Center | 801 Forest Ave, Des Moines, IA 50314 | Center |
| CFI Teddy Bear Town | 3725 52nd St, Des Moines, IA 50310 | Center |
| Capitol Park Early Learning Center | 800 E 12th St, Des Moines, IA 50316 | Center |
| Capitol Park Early Learning Center - EAST HIGH | 1421 Walker St, Des Moines, IA 50316 | Center |
| Casady Early Learning Cemter | 1801 16th St, Des Moines, IA 50314 | Center |
| Casey's General Stores Child Development Center | 1 SE Convenience Blvd, Ankeny, IA 50021 | Center |
| Child's View Day Care | 118 SW State St, Ankeny, IA 50023 | Center |
| Childserve Child Care Center | 5609 Greendale Rd, Johnston, IA 50131 | Center |
| Childserve Woodland Center | 644 24th St, Des Moines, IA 50312 | Center |
| Childtime of Ankeny - Closed | 718 NE Grant Street, Ankeny, IA 50021 | Center |
| Church Of The Cross Preschool | 1975 8th St SW, Altoona, IA 50009 | Center |
| Conmigo Early Education Center | 828 Scott Ave, Des Moines, IA 50309 | Center |
| Cottontail Creek Montessori School | 7297 NW Fisher Lane, Ankeny, IA 50023 | Center |
| Cowles Children's Center | 6401 College Ave, Des Moines, IA 50309 | Center |
| Creative Center For Young Children | 1001 Pleasant St, Des Moines, IA 50309 | Center |
| Creative Children Preschool | 2506 SW 3rd St, Ankeny, IA 50023 | Center |
| Creative Kids Learning Center | 1535 SE Delaware Ave, Ankeny, IA 50021 | Center |
| DMACC Child Development Center | 2006 S ANKENY BLVD BLDG 9, Ankeny, IA 50023 | Center |
| Des Moines CSD - M.E.E.P. - Edmunds | 1601 Crocker Street, Des Moines, IA 50314 | Center |
| Des Moines Day Care | 555 5th Avenue, Des Moines, IA 50309 | Center |
| Discovery World Preschool | 914 NW Ash Dr, Ankeny, IA 50023 | Center |
| Drake University Early Head Start - Highland Park | 4260 NW 6th Dr, Des Moines, IA 50313 | Center |
| Evangelical Covenant Preschool | 2625 Hubbell Ave, Des Moines, IA 50317 | Center |
| Evelyn Davis Early Learning Academy | 1409 Clark St Ste 7, Des Moines, IA 50314 | Center |
| Exemplary Early Learning Center | 901 Walnut St, Des Moines, IA 50309 | Center |
| Findley Elem Head Start | 3000 Cambridge St, Des Moines, IA 50313 | Center |
| First Assembly of God Child Development Center | 2725 Merle Hay Rd, Des Moines, IA 50310 | Center |
| First Steps Child Care Center of Grimes | 441 Gateway Drive, Grimes, IA 50111 | Center |
| Generation Next | 6109 Merle Hay Rd, Johnston, IA 50131 | Center |
| Grace Kids Care Center | 3010 52nd St, Des Moines, IA 50310 | Center |
| Grace Preschool and Drop-In Center | 3700 Cottage Grove Ave, Des Moines, IA 50311 | Center |
| Grand View Child Development Center | 3451 Hubbell Ave, Des Moines, IA 50317 | Center |
| Grand View Child Development Center II | 3004 E 38th St, Des Moines, IA 50317 | Center |
| Holy Family School - Latch Key - Des Moines | 1265 E 9Th St, Des Moines, IA 50316 | Center |
| Hope Lutheran Preschool | 3857 E 42nd St, Des Moines, IA 50317 | Center |
| House Of Mercy - John R Grubb Child Daycare | 1409 Clark St, Des Moines, IA 50314 | Center |
| Iowa Health Child Development Center | 1019 Chestnut, Des Moines, IA 50309 | Center |
| Kids Academy Of Bondurant | 119B Brick St, Bondurant, IA 50035 | Center |
| Kids Connection - Holy Trinity School | 2922 Beaver Ave, Des Moines, IA 50310 | Center |
| Kids Teen Connection | Johnston Comm Sch, Johnston, IA 50131 | Center |
| Kids Towne USA SWTC Child Care Center | 1800 Bronson BLVD, Des Moines, IA 50319 | Center |
| KinderFarm Preschool | 5048B Highway 6 SE, Iowa City, IA 52240 | Center |
| Lil Scholars Preschool | 756 9th Street, Des Moines, IA 50309 | Center |
| Little Endeavors | 205 SE Oralabor Rd, Ankeny, IA 50021 | Center |
| Little Lambs Preschool | 5625 Franklin Ave, Des Moines, IA 50310 | Center |
| Loving Arms Children's Center | 6270 Merle Hay Rd, Johnston, IA 50131 | Center |
| MEEP | 1620 Pleasant St, Des Moines, IA 50314 | Center |
| Mckee Learning Center | 2115 E 39Th St, Des Moines, IA 50317 | Center |
| Mckinley Universal Preschool | 1610 Se 16Th St, Des Moines, IA 50315 | Center |
| Mercy Child Dev Center | 1150 6th Ave, Des Moines, IA 50314 | Center |
| Meredith Drive Preschool- Bridge Campus | 10025 NW 62nd Ave, Des Moines, IA 50310 | Center |
| Metro Kids - Brubaker | 2900 E 42nd St, Des Moines, IA 50317 | Center |
| Metro Kids - Capitol View | 320 E 16th St, Des Moines, IA 50316 | Center |
| Metro Kids - Carver - Cattell - Findley - Madison | 3101 E 12th Street, Des Moines, IA 50316 | Center |
| Metro Kids - Cowles | 6401 College Ave, Windsor Heights, IA 50324 | Center |
| Metro Kids - Garton | 2820 E 24th St, Des Moines, IA 50317 | Center |
| Metro Kids - Hillis | 2401 56th St, Des Moines, IA 50310 | Center |
| Metro Kids - Hubbell | 800 42nd St, Des Moines, IA 50312 | Center |
| Metro Kids - King - Monroe | 2250 30th St, Des Moines, IA 50310 | Center |
| Metro Kids - Oak Park | 3928 6th Ave, Des Moines, IA 50313 | Center |
| Metro Kids - Perkins | 4301 College Ave, Des Moines, IA 50311 | Center |
| Metro Kids - Phillips | 1701 Lay St, Des Moines, IA 50317 | Center |
| Metro Kids - Pleasant Hill | 4801 E Oakwood Dr, Des Moines, IA 50327 | Center |
| Metro Kids - Samuelson | 3929 Bel Aire, Des Moines, IA 50310 | Center |
| Metro Kids - Stowe | 1411 E 33rd Street, Des Moines, IA 50317 | Center |
| Metro Kids - Walnut Street | 901 Walnut St, Des Moines, IA 50309 | Center |
| Metro Kids - Willard | 2941 Dean Ave, Des Moines, IA 50317 | Center |
| Metro Kids - Windsor | 5912 University Ave, Des Moines, IA 50311 | Center |
| New Life Center Daycare | 1029 23rd St, Des Moines, IA 50311 | Center |
| Oakridge Early Enrichment Child Care Center | 1401 Center Street, Des Moines, IA 50314 | Center |
| Our Lady's Little Learners Preschool | 510 E 1st St, Ankeny, IA 50021 | Center |