| Alafaya Woods KinderCare | 300 Alafaya Woods Blvd, Oviedo, FL 32765 | Preschool |
| Aloma Methodist Early Childhood Learning Center | 3045 Aloma Avenue, Winter Park, Florida, Winter Park, FL 32792 | Preschool |
| Childtime of Oviedo | 1500 Swanson Drive, Oviedo, FL 32765 | Preschool |
| Childtime of Winter Park | 1001 South Semoran Boulevard, Winter Park, FL 32792 | Preschool |
| Goldenrod Road KinderCare | 4035 N Goldenrod Rd, Winter Park, FL 32792 | Preschool |
| KinderCare Orlando | 6537 Vista Park Blvd, Orlando, FL 32829 | Preschool |
| La Petite Academy of Orlando | 5066 S Conway Rd, Orlando, FL 32812 | Preschool |
| La Petite Academy of Orlando | 401 Mark Twain Blvd, Orlando, FL 32828 | Preschool |
| La Petite Academy of Orlando | 7303 Lake Underhill, Orlando, FL 32822 | Preschool |
| Winter Springs KinderCare | 1024 Spring Villas Pt, Winter Springs, FL 32708 | Preschool |
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| 4-C Seminole Head Start - Lawton | 188 South Lake Jessup Road, Oviedo, FL 32765 | Center |
| A Child's Place | 2301 E Michigan St, Orlando, FL 32806 | Preschool |
| A Growing Place Child Care | 18555 Old Cheney Hwy, Orlando, FL 32820 | Preschool |
| A Kid's Gym Fitness Center | 1495 Evans Street, Oviedo, FL 32765 | Preschool |
| ABC Learning Center and Child Care | 2310 East Concord Street, Orlando, FL 32803 | Preschool |
| Advanced Beginnings Childcare Development | 406 E. Amelia Street, Orlando, FL 32803 | Preschool |
| All About Kidz of Oviedo | 387 West Broadway Street, Oviedo, FL 32765 | Preschool |
| All Saints School | 338 East Lyman Avenue, Winter Park, FL 32789 | Preschool |
| Aloma Elementary School Preschool | 2949 Scarlet Road, Winter Park, FL 32789 | Preschool |
| Aloma Kids Academy | 3416 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Aloma Kids of Orlando | 3416 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Altamonte Learning Center | 1329 Altamonte Drive, Casselberry, FL 32707 | Preschool |
| American Pre-schools | 4000 South Goldenrod Road, Orlando, FL 32822 | Preschool |
| Angel Sprouts Academy | 7651 Valencia College Lane, Orlando, FL 32807 | Preschool |
| Ascension Lutheran Early Childhood Program | 351 Ascension Drive, Casselberry, FL 32707 | Preschool |
| Atonement Lutheran Preschool | 8849 Passaic Parkway, Orlando, FL 32829 | Center |
| Audubon Elementary School Preschool | 1750 Common Way Rd, Orlando, FL 32814 | Preschool |
| Aunt Delores's Child Care | 1411 Melanie Drive, Orlando, FL 32825 | Preschool |
| Avalon Elementary School Preschool | 13500 Tanji King Blvd, Orlando, FL 32828 | Preschool |
| Azalea Park Baptist School | 5725 Dahlia Dr, Orlando, FL 32807 | Preschool |
| Azalea Park Elementary School Preschool | 1 Carol Avenue, Orlando, FL 32807 | Preschool |
| Azalea Park Learning Center | 940 South Semoran Boulevard, Orlando, FL 32807 | Preschool |
| BETA Center Developmental Childcare | 4680 Lake Underhill Rd, Orlando, FL 32807 | Preschool |
| Baldwin Oaks Academy | 1862 Winter Park Road, Orlando, FL 32803 | Center |
| Best Memories Academy Child Care Center | 5135 Curry Ford Road, Orlando, FL 32812 | Preschool |
| Big Children's Academy | 3300 S. Conway Road, Orlando, FL 32812 | Preschool |
| Bithlo Head Start | 18501 Washington Avenue, Orlando, FL 32820 | Preschool |
| Black Belt Center of Orlando | 4516 Hoffner Ave, Orlando, FL 32812 | Center |
| Bright Horizons at Baldwin Park | 1700 Fire House Lane, Orlando, FL 32814 | Preschool |
| Brighter Futures Enrichment & Learning Center | 221 East Kennedy Boulevard, Maitland, FL 32751 | Preschool |
| Brush Arbor Christian | 2304 North Goldenrod Road, Orlando, FL 32807 | Center |
| CHS A Place For Children | 425 North Orange Avenue R270 S ..., Orlando, FL 32801 | Center |
| Callahan Headstart | 101 North Parramore Avenue, Orlando, FL 32801 | Preschool |
| Camelot Elementary School Preschool | 14501 Waterford Chase Parkway, Orlando, FL 32828 | Preschool |
| Casselberry Elementary Vpk - Seminole Co | 1075 Crystal Bowl Circle, Casselberry, FL 32707 | Preschool |
| Castle Creek Elementary School Preschool | 1245 N. Avalon Park Blvd, Orlando, FL 32828 | Preschool |
| Center For Early Childhood Education | 341 North Orlando Avenue, Maitland, FL 32751 | Preschool |
| Central Florida Christian Preschool | 2218 Alafaya Trail, Oviedo, FL 32765 | Preschool |
| Cheney Elementary School Preschool | 2000 N. Forsyth Road, Orlando, FL 32807 | Preschool |
| Chicksaw Elementary School Preschool | 6900 Autumnvale Dr., Orlando, FL 32822 | Preschool |
| Childlife Preschool | 5495 Lake Margaret Drive, Orlando, FL 32812 | Preschool |
| Children 1st Preschool | 11648 McCulloch Rd, Orlando, FL 32817 | Center |
| Children's Academy at Loch Haven | 200 E Evans St, Orlando, FL 32804 | Preschool |
| Children's Academy at Loch Haven - Mildly Ill | 227 East Evans Street, Orlando, FL 32804 | Preschool |
| Children's Prep Academy | 1883 S. Volusia Avenue, Oviedo, FL 32762 | Preschool |
| Chuck and Kerry's House | 234 Concord Drive, Casselberry, FL 32707 | Preschool |
| Coalition For The Homeless | 639 W Central Blvd, Orlando, FL 32801 | Preschool |
| College Park U M Child Dev Center | 44 West Princeton Street, Orlando, FL 32804 | Preschool |
| Columbia Elementary School Preschool | 18501 Cypress Lake Glen, Orlando, FL 32801 | Preschool |
| Community Alliance Preschool | 4815 E Lake Dr, Winter Springs, FL 32708 | Preschool |
| Community Christian Preschool | 4921 South U.s. Highway 17-92, Casselberry, FL 32707 | Preschool |
| Community Church Preschool | 3839 S Ferncreek Ave, Orlando, FL 32806 | Center |
| Conway Learning Center | 3636 E Michigan St, Orlando, FL 32812 | Preschool |
| Conway United Methodist Preschool | 3401 S Conway Rd, Orlando, FL 32812 | Preschool |
| Denton Johnson Head Start | 400 Ruffle Street, Maitland, FL 32751 | Preschool |
| Discover Academy | 319 South Lakewood Drive, Orlando, FL 32803 | Preschool |
| Discovery Zone Preschool | 11333 Lake Underhill Rd, Orlando, FL 32825 | Preschool |
| Discovery Zone ll Preschool | 2876 S. Alafaya Trail, Orlando, FL 32828 | Preschool |
| Divine Individuals Developmental Center | 140 South West Avenue, Maitland, FL 32751 | Preschool |
| Downey Christian Pre-School | 10201 E. Colonial Drive, Orlando, FL 32817 | Preschool |
| Dreams Castle | 1530 South Primrose Drive, Orlando, FL 32806 | Preschool |
| Early Childhood Center - First United Methodist | 263 King St, Oviedo, FL 32765 | Preschool |
| East Lake Elementary School Preschool | 3971 N Tanner Rd, Orlando, FL 32826 | Preschool |
| East Orange Head Start | 12050 East Colonial Drive, Orlando, FL 32826 | Preschool |
| Eastbrook Elementary Vpk - Seminole County | 5525 Tangerine Avenue, Winter Park, FL 32792 | Preschool |
| Eastland Christian School | 9000 Lakeunder Hill Road, Orlando, FL 32825 | Center |
| Einstein Academy - Campus 2 | 12046 Collegiate Way, Orlando, FL 32817 | Preschool |
| Einstein Academy at La Vina | 9145 Narcoossee, Orlando, FL 32827 | Preschool |
| Engelwood Elementary School Preschool | 900 Engel Dr, Orlando, FL 32807 | Preschool |
| English Estates Pre-k | 299 Oxford Road, Casselberry, FL 32730 | Preschool |
| Evans Elementary Vpk Preschool | 100 Chapman Road, Oviedo, FL 32765 | Preschool |
| Faith Christian Academy Preschool | 2008 N Goldenrod Road, Orlando, FL 32807 | Center |
| Fern Creek Elementary School Preschool | 1121 N. Fern Creek Ave., Orlando, FL 32803 | Preschool |
| First Congregational Church Preschool | 225 South Interlachen Avenue, Winter Park, FL 32789 | Preschool |
| First Friends | 789 N. Alafaya Tr, Orlando, FL 32828 | Preschool |
| First Phase Children's Academy | 116 N Bumby Ave, Orlando, FL 32803 | Preschool |
| First Presbyterian Church Infant Child Care Center | 139 Jackson Street, Orlando, FL 32801 | Center |
| First School Day Care | 7309 E Pershing Ave, Orlando, FL 32822 | Center |
| First Steps Pre-School and Development Center | 12727 East Colonial Drive, Orlando, FL 32826 | Preschool |
| First Steps Preschool & Daycare | 1199 Clay Street, Winter Park, FL 32789 | Preschool |
| First Steps of Learning Camp Seminole | 390 Piney Ridge Road, Casselberry, FL 32707 | Preschool |
| First Years Preschool and Kindergarten | 45 W Broadway St, Oviedo, FL 32765 | Center |
| Golden Bear Academy | 421 S Goldenrod Rd, Orlando, FL 32822 | Preschool |
| Good Shepherd Early Childhood Educational Center | 5902 Oleander Dr, Orlando, FL 32807 | Preschool |
| Grand Avenue Elementary School Preschool | 800 W. Grand St., Orlando, FL 32805 | Preschool |
| Growing Minds Educational Day Care Center | 11887 E. Colonial Dr., Orlando, FL 32826 | Center |
| Growing Together Academy | 843 Woodbury Rd, Orlando, FL 32828 | Preschool |
| Growing Tree Learning Center | 900 Dodd Road, Casselberry, FL 32707 | Preschool |
| Hand N Hand Child Enrichment Center | 6225 Hazeltine National Drive, Orlando, FL 32822 | Preschool |
| Happyland Learning Academy | 1295 North Semoran Boulevard, Orlando, FL 32807 | Preschool |