| Sunny Days After School | 12825 Hashanli Place, Matthews, NC 28105 | Center |
| Sunsetwood Academy | 1304 Eastway Drive, Charlotte, NC 28205 | Preschool |
| Sunshine House Early Learning Academy - Matthews | 1208 Sam Newell Road, Matthews, NC 28105 | Preschool |
| Superlative Academy | 1238 East 10th Street, Charlotte, NC 28204 | Center |
| Taylor's Home Day Care | 6214 Mayridge Dr, Charlotte, NC 28215 | Center |
| Teachers' Pet | 406 Silversmith Ln, Charlotte, NC 28270 | Preschool |
| Tegge's Learning Christian Enrichment Center | 2214 N Sharon Amity Road, Charlotte, NC 28205 | Center |
| Tele - Time Academy | 3426 Central Ave, Charlotte, NC 28205 | Center |
| Tender Hearts Child Care | 2301 Sharon Amity Rd N, Charlotte, NC 28205 | Center |
| The Charlotte Preparatory School | 212 Boyce Road, Charlotte, NC 28211 | Preschool |
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| The Learning Collaborative | 3045 North Davidison Street, Charlotte, NC 28205 | Preschool |
| The Learning Tree Enrichment Academy | 5500 Starkwood Drive, Charlotte, NC 28212 | Preschool |
| The Sunshine House - Mint Hill | 5825 Phyliss Lane, Charlotte, NC 28227 | Center |
| Tiaes And Joshua's Christian Playschool | 9827 Veronica Dr, Charlotte, NC 28215 | Center |
| Tiny Tots 2 Childcare | 2100 Eastway Drive, Charlotte, NC 28205 | Center |
| Toddlers University | 7231 Fox Hunt Road, Charlotte, NC 28212 | Center |
| Toddletown Kidz | 7328 Mossborough Ct, Charlotte, NC 28227 | Center |
| Tots Round The Clock | 3639 Eastway Dr, Charlotte, NC 28205 | Center |
| Tots World | 4432 Gaynelle Dr, Charlotte, NC 28215 | Center |
| University Child Development Center | 8303 University Executive Park, Charlotte, NC 28262 | Preschool |
| University Meadows After School Enrichment Program | 1600 Pavilion Blvd, Charlotte, NC 28262 | Center |
| Vision Love Day Care Home | 5424 Dennis Ct, Charlotte, NC 28213 | Center |
| Westminster Presbyterian - SUNDAY SCHOOL ONLY | 101 Collville Road, Charlotte, NC 28207 | Center |
| Winterfield Elementary ASEP | 3100 Winterfield Place, Charlotte, NC 28205 | Center |