| 21st Century Homework Club | 907 Kingsprings Road, Johnson City, TN 37601 | Center |
| Bluff City Elementary Pre-kindergarten | 282 J. Forrest Thomas Street, Bluff City, TN 37618 | Preschool |
| Bluff City Head Start | 282 J. Forest Thomas St., Bluff City, TN 37618 | Preschool |
| Carver Head Start | 500 Washington St, Johnson City, TN 37601 | Center |
| Central Baptist Preschool | 300 North Roan Street, Johnson City, TN 37601 | Preschool |
| Central Elementary - Head Start | 252 Taylor Town Rd., Johnson City, TN 37601 | Preschool |
| Central Elementary Carter Cares | 252 Taylortown Road, Johnson City, TN 37601 | Center |
| Cherokee Christian Learning Center | 2615 South Roan Street, Johnson City, TN 37601 | Preschool |
| Children First Head Start Center | 837 Pardee St., Johnson City, TN 37601 | Preschool |
| Children's Center Of Sycamore Shoals Hospital | 408 Bemberg Road, Elizabethton, TN 37643 | Center |
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| Coalition For Kid's - Kid's In Action | 2308 Watauga Rd., Johnson City, TN 37601 | Center |
| Coalition For Kids Mountain View Elementary | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Coalition For Kids North Side Elementary | 1000 N. Roan St., Johnson City, TN 37601 | Center |
| Community Day Care & Learning Center | 820 Hemlock St., Elizabethton, TN 37643 | Center |
| Community Day Care & Learning Center | 210 South Hills Dr, Elizabethton, TN 37643 | Center |
| Crystal's Cuties | 1497 Southside Rd., Elizabethton, TN 37643 | Center |
| East Side Day Care & Learning Center | 206 Old Siam Road, Elizabethton, TN 37643 | Center |
| East Side Elementary Pre-kindergarten | 800 Siam Road, Elizabethton, TN 37643 | Preschool |
| Elizabethton Boys And Girls Club | 104 Hudson Drive, Elizabethton, TN 37643 | Center |
| Fairmont Elementary - Educare | 1405 Lester Harris Road, Johnson City, TN 37601 | Center |
| First Christian Church Preschool | 200 Mountcastle Drive, Johnson City, TN 37601 | Preschool |
| First United Methodist Preschool | 325 East E Street, Elizabethton, TN 37643 | Preschool |
| Hampton Elementary Carter Cares After | 408 Highway 321, Hampton, TN 37658 | Center |
| Hampton Elementary Head Start | 408 Highway 321, Hampton, TN 37658 | Preschool |
| Hampton Elementary Head Start II | 408 Highway 321, Hampton, TN 37658 | Preschool |
| Happy Valley Elementary Head Start | 1840 Milligan Highway, Johnson City, TN 37601 | Preschool |
| Happy Valley Elementary School - Extended School Program | 1840 Milligan Highway, Johnson City, TN 37601 | Center |
| Happy Valley Middle School Leaps | 163 Warpath Lane, Elizabethton, TN 37643 | Center |
| Harold Mccormick - Extended School Program | 226 Cedar Avenue, Elizabethton, TN 37643 | Center |
| Hunter Elementary Head Start | 145 Hope Street, Elizabethton, TN 37643 | Preschool |
| Hunter First Baptist Daycare & Learning Center I | 693 Highway 91, Elizabethton, TN 37643 | Center |
| Hunter First Baptist Daycare & Learning Center II | 693 Highway 91, Elizabethton, TN 37643 | Center |
| Johnson City Prek At Fairmont Elementary | 1405 Lester Harris Road, Johnson City, TN 37601 | Preschool |
| Joy In Learning | 489 Egypt Rd., Bluff City, TN 37618 | Center |
| Keenburg Carter Cares | 139 Keenburg Road, Elizabethton, TN 37643 | Center |
| Keenburg Elementary Head Start | 139 Keenburg Road, Elizabethton, TN 37643 | Preschool |
| Lake Terrace Head Start | 2450 Mint Hill Rd, Johnson City, TN 37601 | Preschool |
| Little Kids Country | 187 Hardin Hollow Road, Elizabethton, TN 37643 | Center |
| Little Lambs Learning Center And Day Care | 1207 Broad St., Elizabethton, TN 37643 | Preschool |
| Mountain View Baptist Daycare & Learning | 4005 Bristol Hwy., Johnson City, TN 37602 | Center |
| Mountain View Elementary Educare | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Munsey Memorial Preschool | 201 E. Market St., Johnson City, TN 37601 | Preschool |
| North Side Elementary School Educare | 1000 North Roan Street, Johnson City, TN 37601 | Center |
| Northside Elementary Pre-kindergarten | 1000 N. Roan, Johnson City, TN 37601 | Preschool |
| Pinecrest Head Start | 500 Angel Drive, Johnson City, TN 37601 | Preschool |
| Piney Bluff Childcare | 4429 Bluff City Hwy, Bluff City, TN 37618 | Center |
| Playstation - The Kids Depot | 1006 South Broadway, Johnson City, TN 37601 | Center |
| Princeton Preporatory | 504-a Princeton Road, Johnson City, TN 37601 | Center |
| Range Elementary Carter Cares | 655 Watauga Road, Watauga, TN 37694 | Center |
| Range Elementary School Pre-kindergarten | 655 Watauga Road, Watauga, TN 37694 | Preschool |
| Rise Up! | 1500 East Millard Street, Johnson City, TN 37601 | Center |
| Science Hill Preschool | Technology Center, Johnson City, TN 37601 | Preschool |
| Shining Stars Christian Learning | 2615 South Roan Street, Johnson City, TN 37604 | Center |
| Small Steps Children's Academy | 1201 East Chilhowie Ave., Johnson City, TN 37601 | Center |
| St. Mary's School | 2211 East Lakeview Drive, Johnson City, TN 37601 | Center |
| Stepping Stones Day Care And Preschool | 216 East Market St., Johnson City, TN 37601 | Preschool |
| Telamon Early Head Start | 1207 Broad Street, Elizabethton, TN 37643 | Center |
| Tennessee Tots Learning Center | 440 Blue Ridge Drive, Elizabethton, TN 37643 | Center |
| Toddler Time Day Care Center | 435 Highway 321, Hampton, TN 37658 | Center |
| Unaka Elementary Carter Cares | 120 Unaka Drive, Elizabethton, TN 37643 | Center |
| Valley Forge Carter Cares | 1485 Riverview Drive, Elizabethton, TN 37643 | Center |
| Valley Forge Elementary Head Start II | 485 Riverview Road, Elizabethton, TN 37643 | Preschool |
| Valley Forge Elementary Pre-kindergarten | 1485 Riverview Drive, Elizabethton, TN 37643 | Preschool |
| Wesley Memorial United Methodist Church Preschool | 225 Princeton Road, Johnson City, TN 37601 | Preschool |