| Childtime of Winston Salem | 1021 West Clemmonsville Road, Winston Salem, NC 27127 | Preschool |
| La Petite Academy of Kernersville | 1975 West Mountain Street, Kernersville, NC 27284 | Preschool |
| La Petite Academy of Winston Salem | 2011 Bethabara Road, Winston Salem, NC 27106 | Preschool |
| La Petite Academy of Winston Salem | 110 Jonestown Road, Winston Salem, NC 27104 | Preschool |
| The Children's Courtyard of Kernersville | 1009 Masten Dr, Kernersville, NC 27284 | Center |
| A Better World Learning Center | 317 E 33rd St, Winston Salem, NC 27105 | Center |
| A Child's World Learning Center | 1290 Hartman Plaza Drive, Winston Salem, NC 27127 | Preschool |
| A Child's World Learning Center | 702 N Cherry Street, Winston Salem, NC 27101 | Preschool |
| Beck's Baptist Church Day Care | 5505 Beck's Church Road, Winston Salem, NC 27106 | Preschool |
| Bethlehem Community Child Development Center | 520 Cleveland Ave, Winston Salem, NC 27101 | Center |
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| Beulahland Child Development Center | 1352 North Trade Street, Winston Salem, NC 27105 | Center |
| Bolton Elementary Prekindergarten Program | 1250 Bolton Street, Winston Salem, NC 27103 | Center |
| Brookside Montessori School | 736 Piney Grove Road, Kernersville, NC 27284 | Center |
| Buchanan Head Start Center | 2555 Buchanan St, Winston Salem, NC 27127 | Center |
| Caesar's Lil Palace | 5720 Shattalon Drive, Winston Salem, NC 27105 | Center |
| Carver Road Educational & Development Center | 3111 Carver School Road, Winston Salem, NC 27105 | Center |
| Cash Elementary Prekindergarten Program | 4700 Old Hollow Road, Kernersville, NC 27284 | Center |
| Cedar Forest Baptist Day Care | 4634 Shattalon Drive, Winston Salem, NC 27106 | Center |
| Centenary Child Care Center | 646 West Fifth Street, Winston Salem, NC 27102 | Center |
| Childcare Network #161 | 820 Salisbury Street, Kernersville, NC 27284 | Preschool |
| Childcare Network #299 | 3595 Reynolda Rd., Winston Salem, NC 27106 | Center |
| Children's Center For The Physically Disabled | 2315 Coliseum Drive, Winston Salem, NC 27106 | Center |
| Children's Choice Learning Center | 200 Lockland Ave, Winston Salem, NC 27103 | Preschool |
| Christ Rescue Temple #3 | 1625 N Dunleith Ave, Winston Salem, NC 27105 | Center |
| Church Child Care Center | 4400 Poindexter Street, Walkertown, NC 27051 | Preschool |
| Country Club Learning & Development Center | 3842 Country Club Road, Winston Salem, NC 27104 | Center |
| Creative Day School | 3595 Reynolda Road, Winston Salem, NC 27106 | Preschool |
| Discovering Our World Child Development Center | 3640 New Walkertown Road, Winston Salem, NC 27105 | Preschool |
| Early Image Child Care Center - FTHC | 3749 New Walkertown Rd, Winston Salem, NC 27105 | Center |
| Easton Elementary Prekindergarten Program | 734 Clemmonsville Road, Winston Salem, NC 27107 | Preschool |
| Educating Angels Child Care Center | 5909 Germanton Rd, Winston Salem, NC 27105 | Center |
| Ephesus Learning Center | 1225 North Cleveland Avenue, Winston Salem, NC 27101 | Center |
| Epiphany Early Childhood Center | 5220 Silas Creek Pkwy, Winston Salem, NC 27106 | Center |
| Faith Day Care Center | 1077 Gumtree Road, Winston Salem, NC 27107 | Center |
| Family Services Child Development at WSSU | 601 S Martin Luther King Jr Dr, Winston Salem, NC 27110 | Preschool |
| Family Services, | 2050 Bighouse Gaines Blvd, Winston Salem, NC 27101 | Preschool |
| First Assembly Preschool #1 | 3730 University Pkwy, Winston Salem, NC 27106 | Preschool |
| First Baptist Church | 401 Oakhurst Street, Kernersville, NC 27284 | Preschool |
| First Baptist Church Children's Center | 501 West Fifth Street, Winston Salem, NC 27101 | Preschool |
| First Step Preschool | 1305 Pleasant Fork Church Road, Winston Salem, NC 27127 | Preschool |
| Forest Park Elementary School Prekindergarten Program | 2019 Milford Street, Winston Salem, NC 27107 | Center |
| Friedland Moravian Church Day Care | 2750 Friedland Church Rd, Winston Salem, NC 27107 | Center |
| Future Leaders Learning Academy | 725 Waughtown Street, Winston Salem, NC 27107 | Center |
| Future Young Scholars Child Development Center | 1398 Jonestown Road, Winston Salem, NC 27103 | Center |
| Generations Preschool And Child Care | 4465 Bridalcreek West, Winston Salem, NC 27106 | Preschool |
| Gospel Light Christian School | 4940 Gospel Light Church Road, Winston Salem, NC 27101 | Center |
| Guys And Dolls Child Care | 220 Terrace Ave, Winston Salem, NC 27101 | Center |
| Happy Days Child Care | 1480 Harvest Drive, Winston Salem, NC 27101 | Center |
| Higher Learning Child Development Center | 2081 Kerensky St, Winston Salem, NC 27103 | Center |
| Holy Cross Child Development Center | 616 South Cherry Street, Kernersville, NC 27284 | Center |
| K J's Kiddie Land | 1715 Brewer Road, Winston Salem, NC 27127 | Center |
| Kelly's Learning Center | 1000 East Fourth Street, Winston Salem, NC 27101 | Center |
| Kidz Zone Learning Center | 515 Polo Road, Winston Salem, NC 27106 | Center |
| Kimberley Park Elementary School Prekindergarten Program | 1701 North Cherry Street, Winston Salem, NC 27105 | Center |
| Konnoak Elementary Extended Day Program | 3200 Renon Road, Winston Salem, NC 27127 | Center |
| Little Red School House | 2842 Old Greensboro Road, Winston Salem, NC 27101 | Center |
| Little Snuggles Christian Center | 3862 Reynolda Road, Winston Salem, NC 27106 | Center |
| Lollipop Stop Child Development Center | 160 Noel Drive, Winston Salem, NC 27105 | Center |
| Main Street Baptist Day Care | 126 N Main Street, Kernersville, NC 27284 | Center |
| Marvin M. Ward Elementary Prekindergarten Program | 37775 Fraternity Church Road, Winston Salem, NC 27127 | Center |
| Maxx Kinder College | 2411 Waughtown Street, Winston Salem, NC 27107 | Center |
| Middle Fork Elementary Prekindergarten Program | 3125 Williston Road, Walkertown, NC 27051 | Center |
| Mineral Springs Baptist Child Care | 3733 Ogburn Ave, Winston Salem, NC 27105 | Center |
| Mineral Springs Head Start | 4527 Ogburn Ave, Winston Salem, NC 27105 | Preschool |
| Mt. Sinai Child Development Center | 4634 Grove Garden Drive, Winston Salem, NC 27105 | Center |
| Mt. Zion Child Development Center | 950 File Street, Winston Salem, NC 27101 | Center |
| Mud Pies Child Development - Bolton | 1210 Bolton St, Winston Salem, NC 27103 | Preschool |
| Mud Pies Child Development Center - Coliseum | 2530 Pittsburgh Ave, Winston Salem, NC 27102 | Preschool |
| Mud Pies Child Development Centers - Downtown | 300 N Poplar Street, Winston Salem, NC 27101 | Preschool |
| Nona's Day Care Center | 3219 Tracy St, Winston Salem, NC 27105 | Center |
| North Point Academy | 4950 Indiana Avenue, Winston Salem, NC 27105 | Center |
| Oak Forest After School Program | 11461 Old Us Highway 52, Winston Salem, NC 27107 | Center |
| Oak Summit Learning Academy | 230 Oak Summit Road, Winston Salem, NC 27105 | Preschool |
| Our Lady Of Mercy Preschool | 1730 Link Road, Winston Salem, NC 27103 | Preschool |
| Parkview Christian Day Care | 3263 Kernersville Road, Winston Salem, NC 27107 | Preschool |
| Peek - A - Moo Child Development Center | 1001reynolda Road, Winston Salem, NC 27104 | Preschool |
| Pinedale Christian Day Care | 3395 Peters Creek Parkway, Winston Salem, NC 27127 | Center |
| Playtime Day Care And Early Learning Center | 5890 Sullivantown Road, Walkertown, NC 27051 | Center |
| Primrose School Of Kernersville | 1009 Masten Dr, Kernersville, NC 27284 | Preschool |
| Salem Baptist Christian School | 429 South Broad Street, Winston Salem, NC 27101 | Preschool |
| Sandbox Academy Child Care Center | 5016 Old Walkertown Road, Winston Salem, NC 27105 | Center |
| School Of Grace | 2236 Old Sailisbury Road, Winston Salem, NC 27127 | Center |
| Sharpe Kidz | 108 Davis Street, Kernersville, NC 27284 | Center |
| Small Angels Childcare | 2425 Old Salisbury Road, Winston Salem, NC 27127 | Center |
| Sniffles N' Such | 3333 Silas Creek Parkway, Winston Salem, NC 27103 | Center |
| Speas Elementary Prekindergarten Program | 2000 West Polo Rd, Winston Salem, NC 27106 | Preschool |
| Special Children's School | 4505 Shattalon Drive, Winston Salem, NC 27106 | Preschool |
| St. Andrews Day Care Center | Hwy 66, Winston Salem, NC 27105 | Center |
| St. Anne's Day Care | 2690 Fairlawn Drive, Winston Salem, NC 27106 | Center |
| St. John's Lutheran Day Care | 2415 Silas Creek Parkway, Winston Salem, NC 27103 | Preschool |
| Step By Step Day Care | 4612 Old Rural Hall Rd, Winston Salem, NC 27105 | Center |
| Sugar And Spice Child Enrichment Center | 1414 Union Cross Road, Kernersville, NC 27284 | Preschool |
| Sunshine House | 600 East Polo Road, Winston Salem, NC 27106 | Preschool |
| TLC The Learning Academy | 2832 South Main St, Winston Salem, NC 27127 | Center |
| The Bunny Stop Preschool | 4009 Old Vineyard Rd, Winston Salem, NC 27104 | Center |
| The Potter's House For Children | 1035 Hutton Street, Winston Salem, NC 27103 | Center |
| The Sunshine House | 3806 Country Club Road, Winston Salem, NC 27104 | Preschool |
| Today's Child Learning Center | 1005 Bethesda Road, Winston Salem, NC 27103 | Center |
| Triad Baptist Church Child Enrichment Center | 1175 South Main Street, Kernersville, NC 27284 | Preschool |
| Vernon Forest Baptist Church Training Center | 294 Mt Vernon Church Road, Winston Salem, NC 27107 | Center |