| Arlington KinderCare | 3033 Monument Rd Ste 21, Jacksonville, FL 32225 | Center |
| Atlantic Beach KinderCare | 2 Atlantic Ct, Atlantic Beach, FL 32233 | Preschool |
| Gate Parkway KinderCare | 11526 Lake Mead Ave Ste 105, Jacksonville, FL 32256 | Center |
| Intercoastal KinderCare | 11761 Beach Blvd Ste 13, Jacksonville, FL 32246 | Center |
| La Petite Academy of Jacksonville | 50 St. Johns Bluff Road, Jacksonville, FL 32225 | Preschool |
| La Petite Academy of Jacksonville | 13957 Beach Boulevard, Jacksonville, FL 32224 | Preschool |
| La Petite Academy of Jacksonville | 800 Girvin Rd., Jacksonville, FL 32225 | Preschool |
| Ponte Vedra KinderCare | 12000 Sawgrass Village Dr, Ponte Vedra Beach, FL 32082 | Preschool |
| Southside KinderCare | 8595 Beach Blvd Ste 201, Jacksonville, FL 32216 | Center |
| Tutor Time of Jacksonville | 4783 Windsor Commons Court, Jacksonville, FL 32224 | Preschool |
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| 1st Ave Montessori School | 617 First Avenue North, Jacksonville Beach, FL 32250 | Preschool |
| Al Furqan Academy | 2333 St. Johns Bluff Rd, Jacksonville, FL 32246 | Preschool |
| Al-furqan Academy | 2333 St. Johns Bluff Road South, Jacksonville, FL 32246 | Preschool |
| Allen Children Centers | 1804 S. St. Johns Bluff Rd, Jacksonville, FL 32246 | Preschool |
| Apple Patch Learning Center | 14054 Beach Boulevard Suites 1 ..., Jacksonville Beach, FL 32250 | Preschool |
| Apple Patch Learning Center II | 14054 Beach Blvd. Suite 7 & 8, Jacksonville Beach, FL 32250 | Preschool |
| Beach United Methodist Church Early Learning Center | 334 8th Avenue North, Jacksonville Beach, FL 32250 | Preschool |
| Beaches Academy | 1725 Penman Road, Jacksonville Beach, FL 32250 | Preschool |
| Beaches Chapel School | 610 Florida Blvd, Neptune Beach, FL 32266 | Preschool |
| Bethlehem Lutheran Preschool | 1423 8th Ave N, Jacksonville Beach, FL 32250 | Preschool |
| Bethlehem Lutheran Preschool | 1337 Palm Circle, Jacksonville Beach, FL 32250 | Preschool |
| Blessed Trinity Catholic School | 10472 Beach Boulevard, Jacksonville, FL 32246 | Preschool |
| Bright Stars Early Learning Center | 7344 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Calvary Christian Jr. Academy | 3266 Southside Boulevard, Jacksonville, FL 32216 | Preschool |
| Chabad Lubavitch of Southside | 11271 Alumni Way, Jacksonville, FL 32246 | Preschool |
| Chappell at FCCJ South Campus | 11901 Beach Boulevard, Jacksonville, FL 32246 | Preschool |
| Childtime Childrens Center | 45 Executive Way, Ponte Vedra Beach, FL 32082 | Preschool |
| Christ Episcopal Church Preschool | 400 San Juan Drive, Ponte Vedra Beach, FL 32082 | Center |
| Christ Methodist Kindergarten | 400 Penman Road, Neptune Beach, FL 32266 | Preschool |
| Christian Heritage Academy | 3930 University Boulevard South, Jacksonville, FL 32216 | Preschool |
| Circles of Learning by Rose Academy | 8900 Corporate Square Ct., Jacksonville, FL 32216 | Preschool |
| Community Presbyterian Preschool | 150 Sherry Drive, Atlantic Beach, FL 32233 | Preschool |
| Daystar Daycare & Learning Academy | 8061 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Discovery Montessori School | 102 15th Street South, Jacksonville Beach, FL 32250 | Preschool |
| Dream Kids Preschool & Arts Learning Center | 3980 Southside Blvd Ste#102, Jacksonville, FL 32257 | Preschool |
| Exclusively Infants | 11900-225 Atlantic Blvd, Jacksonville, FL 32225 | Center |
| Fort Caroline Learning Tree | 7440 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Franklyn Learning Center | 1851 Beach Boulevard, Jacksonville Beach, FL 32250 | Preschool |
| Ft. Caroline Baptist Academy | 11428 Mccormick Rd., Jacksonville, FL 32225 | Preschool |
| Ft. Caroline Christian Pre-K | 8510 Ft. Caroline Rd., Jacksonville, FL 32277 | Preschool |
| Grace Lutheran School | 12200 Mccormick Road, Jacksonville, FL 32225 | Preschool |
| Head Start at Anders Park | 10770 Anders Blvd #407, Jacksonville, FL 32246 | Preschool |
| Head Start at Lee Road | 1210 Lee Road, Jacksonville, FL 32225 | Preschool |
| Headstart at Southwind Villas | 8711 Newton Road, Jacksonville, FL 32216 | Preschool |
| Hidden Hills Learning Tree | 12160 Ft. Caroline Rd., Jacksonville, FL 32225 | Preschool |
| Highlands Precious Gifts Preschool | 10900 Mccormick Road, Jacksonville, FL 32225 | Preschool |
| Hodges Boulevard Presbyterian Church | 4140 Hodges Boulevard, Jacksonville, FL 32224 | Preschool |
| Holy Spirit Catholic School | 11665 Fort Caroline Road, Jacksonville, FL 32225 | Preschool |
| Intercoastal Kids | 13109 Professional Drive, Jacksonville, FL 32225 | Preschool |
| Isle of Faith Child Development Center | 1821 San Pablo Road, Jacksonville, FL 32224 | Preschool |
| Ivy League Academy at San Pablo | 3232 San Pablo Road South, Jacksonville, FL 32224 | Preschool |
| Jessie Activity Center | 2775-1 Mayport Road, Atlantic Beach, FL 32233 | Preschool |
| Joyful Sounds Christian Academy | 10750 Atlantic Boulevard Suite 11, Jacksonville, FL 32225 | Preschool |
| Kids By The Hour | 13947 Beach Boulevard Suite 210, Jacksonville, FL 32224 | Preschool |
| Kids By The Hour II | 2771 Monument Rd #3, Jacksonville, FL 32225 | Preschool |
| Kingdom Kids at CrossRoad | 10005 Gate Parkway North, Jacksonville, FL 32246 | Preschool |
| La Core Christian Academy | 122 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Lighthouse Christian Preschool | 2115 12th Avenue North, Jacksonville Beach, FL 32250 | Preschool |
| Little Sarephims Daycare | 220 Mill Creek Road, Jacksonville, FL 32211 | Center |
| Little Stars Preschool | 545 A1a North, Ponte Vedra Beach, FL 32082 | Preschool |
| Little Temples Child Care Center II | 9100 Merrill Road Unit 15, Jacksonville, FL 32225 | Preschool |
| Lone Star Learning Center | 8142 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Magellan Christian Academies | 10550 - 704 Deerwood Park Boul ..., Jacksonville, FL 32256 | Preschool |
| Mine Yours & Ours Child Care Learning Center | 7645 Merrill Road #107, Jacksonville, FL 32277 | Preschool |
| Montessori Tides School | 1550 Penman Road, Jacksonville Beach, FL 32250 | Preschool |
| Monument Christian Academy | 1509 Mayport Road, Atlantic Beach, FL 32233 | Center |
| Monument KinderCare - CLOSED | 12040 McCormick Rd, Jacksonville, FL 32225 | Preschool |
| Naval Station Mayport | Building 373 Moale Avenue, Jacksonville, FL 32228 | Preschool |
| Naval Station Mayport Child Development Center | 1137 Cove Landing Drive, Atlantic Beach, FL 32233 | Preschool |
| Neptune Baptist W.E.E. School | 407 3rd Street, Neptune Beach, FL 32266 | Preschool |
| Open Arms Christian Childcare Center | 9039 Beach Boulevard, Jacksonville, FL 32216 | Preschool |
| Palms Preschool & Extended Day Program | 3410 3rd Street South, Jacksonville Beach, FL 32250 | Preschool |
| Parkwood Weekday Early Education Center | 7900 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Providence Child Development Center | 3115 Discovery Way, Jacksonville, FL 32224 | Preschool |
| Saint Paul's Catholic School | 428 2nd Ave. N., Jacksonville Beach, FL 32250 | Preschool |
| Seacoast Christian Academy Preschool | 9570 Regency Square Boulevard, Jacksonville, FL 32225 | Preschool |
| St. Paul Children's Enrichment Center | 8264 Lone Star Road, Jacksonville, FL 32211 | Center |
| T's Learning Center | 11761 Beach Boulevard Suite 13, Jacksonville, FL 32246 | Preschool |
| T's Learning Center II | 8595 Beach Boulevard Unit 201, Jacksonville, FL 32216 | Preschool |
| T's Learning Center IV | 3033 Monument Road Suite 21, Jacksonville, FL 32225 | Preschool |
| The Beaches School | 2049 Florida Boulevard, Neptune Beach, FL 32266 | Preschool |
| The Cambridge Prep School | 12750 Abess Boulevard, Jacksonville, FL 32225 | Preschool |
| The Foundation Academy | 3675 San Pablo Road South, Jacksonville, FL 32224 | Preschool |
| The Goddard School | 14230 Spartina Court, Jacksonville, FL 32224 | Preschool |
| The Red School House | 419 Fifth Avenue North, Jacksonville Beach, FL 32250 | Preschool |
| The Seaside Playgarden | 223 8th Avenue South, Jacksonville Beach, FL 32250 | Preschool |
| Trinity Academy 4 Kids | 3980 Southside Boulevard Units ..., Jacksonville, FL 32216 | Preschool |
| UNF Child Development Research Center | 1 Unf Drive Building 49, Jacksonville, FL 32224 | Preschool |
| Victory Preschool | 4651 Kernan Blvd.s, Jacksonville, FL 32224 | Preschool |
| Windy Hill Elementary Preschool | 3831 Forest Boulevard, Jacksonville, FL 32246 | Preschool |
| Woodland Acres Elementary Preschool | 328 Bowlan Street, Jacksonville, FL 32211 | Preschool |
| YMCA Pryme Time at Panther Enrichment Center | 1709 Landsdowne Drive, Jacksonville, FL 32211 | Center |