| Childtime of Jacksonville | 8500 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Childtime of Orange Park | 1510 Business Center Drive, Fleming Island, FL 32003 | Preschool |
| Eagle Harbor Parkway KinderCare | 1735 Eagle Harbor Pkwy, Fleming Island, FL 32003 | Preschool |
| La Petite Academy of Jacksonville | 6601 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| La Petite Academy of Middleburg | 3150 County Road 220, Middleburg, FL 32068 | Preschool |
| La Petite Academy of Orange Park | 4321 US Highway 17, Fleming Island, FL 32003 | Preschool |
| Oakleaf KinderCare | 431 Drysdale, Orange Park, FL 32065 | Center |
| Tutor Time of Jacksonville | 8510 Watermill Boulevard South, Jacksonville, FL 32244 | Preschool |
| Abbate's Day Care | 349 Blanding Boulevard, Orange Park, FL 32073 | Preschool |
| Advent Lutheran Preschool | 2156 Loch Rane Boulevard, Orange Park, FL 32073 | Preschool |
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| Andromeda Preschool | 131 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| Annunciation Catholic School | 1610 Blanding Blvd., Middleburg, FL 32068 | Preschool |
| Apple Tree Academy Childcare | 1246 Park Avenue, Orange Park, FL 32073 | Center |
| Argyle Christian Preschool and Kindergarten | 6823 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Arora's Clubhouse | 330 Arora Boulevard, Orange Park, FL 32073 | Preschool |
| Asbury Learning Center | 2781 Henley Road, Green Cove Springs, FL 32043 | Preschool |
| Atcom Child Care Learning Center | 5512 Westconnett Blvd, Jacksonville, FL 32244 | Center |
| Backpack University | 400 College Drive, Middleburg, FL 32068 | Center |
| Belmont Head Start | 15 Belmont Blvd, Orange Park, FL 32073 | Preschool |
| Bright Start School and Daycare | 217 Wells Road, Orange Park, FL 32073 | Center |
| Builders of the Faith Christian Daycare | 7540 103rd Street #216, Jacksonville, FL 32210 | Preschool |
| Challenge Enterprises Lighthouse Learning | 550 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Childtime Learning Center | 2890 Moody Avenue, Orange Park, FL 32073 | Preschool |
| Doctors Inlet Elementary Preschool | 2634 Cr 220, Middleburg, FL 32068 | Preschool |
| Episcopal Children's Services Early Learning Center | 2506 Blanding Boulevard, Middleburg, FL 32068 | Preschool |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| First Class Learning Center | 939 Blanding Blvd., Orange Park, FL 32065 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Fleming Island Elementary School Preschool | 4425 Lakeshore Drive, Fleming Island, FL 32003 | Preschool |
| Fox Meadows Chrisitian Daycare | 3227 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Gingerbread Learning Center | 30 Knight Boxx Road, Orange Park, FL 32065 | Preschool |
| Good Samaritan Learning Tree | 3813 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Good Shepherd Lutheran Church Precious Lambs Preschool | 6551 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Grace Episcopal Dayschool | 156 Kingsley Avenue, Orange Park, FL 32073 | Center |
| Grove Park Elementary School Preschool | 1643 Miller Street, Orange Park, FL 32073 | Preschool |
| ISIS LITTLE HOMESCHOOL LLC | 8500 BLACKBERRY LANE EAST, Jacksonville, FL 32244 | Preschool |
| Island View Baptist Child Care Center | 900 Park Avenue, Orange Park, FL 32073 | Preschool |
| Ivy League Academy | 2245 Plantation Center Drive Suite 60, Fleming Island, FL 32003 | Preschool |
| Ivy League Academy at Oak Leaf | 7629 Old Middleburg Road South, Jacksonville, FL 32222 | Preschool |
| JUST KIDS Learning Center | 6247 Ricker Road, Jacksonville, FL 32244 | Preschool |
| Just Kids Learning Center | 120 Parkwood Drive East, Orange Park, FL 32073 | Preschool |
| Kampus Kids | 2300 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Keystone Heights Elementary School Preschool | 335 S. Pecan Street, Orange Park, FL 32065 | Preschool |
| Kid's World Child Center | 5390 County Road 218 West, Middleburg, FL 32068 | Preschool |
| Kids World of Florida | 2121 Constitution Drive, Orange Park, FL 32073 | Preschool |
| KidsFirst Learning Center | 2645 Blanding Blvd, Middleburg, FL 32068 | Preschool |
| Kirkwood Presbyterian Center of Early Learning | 8701 Argyle Forest Blvd, Jacksonville, FL 32244 | Center |
| Lake Asbury Elementary Preschool | 2901 Sandridge Rd, Green Cove Springs, FL 32043 | Preschool |
| Learning Time Academy | 5301 Ricker Road, Jacksonville, FL 32210 | Preschool |
| Little Paws Learning Center | 466 Madison Avenue, Orange Park, FL 32065 | Preschool |
| Little People's Learning Center | 5095 Lakeshore Drive West, Fleming Island, FL 32003 | Preschool |
| Mulberry Lane Childcare | 57 College Drive, Orange Park, FL 32065 | Preschool |
| O.P.K. South of Middleburg | 3425 Habitat Drive, Middleburg, FL 32068 | Center |
| O2B Kids 7 | 1821 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| O2B Kids 7 | 1821 Town Center Blvd. Suite 101, Fleming Island, FL 32003 | Preschool |
| Oak Crest Child Care | 5900 Ricker Rd, Jacksonville, FL 32244 | Preschool |
| Oakleaf Preschool & Childcare | 415 Meldrum Lane, Orange Park, FL 32065 | Preschool |
| Oakleaf Village Elementary Preschool | 410 Oakleaf Village Parkway, Orange Park, FL 32073 | Preschool |
| Ollie's Day Care | 158 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park Kindergarten | 3050 Moody Rd, Orange Park, FL 32065 | Preschool |
| Orange Park Presbyterian Church Preschool | 1905 Park Avenue, Orange Park, FL 32073 | Preschool |
| Orange Park Preschool and Learning Center | 99 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park's Little Miracles | 1981 Wells Rd, Orange Park, FL 32073 | Preschool |
| Ortega Hills Preschool | 5029 Greenway Drive, Jacksonville, FL 32244 | Preschool |
| Parks Place Daycare and Learning Center | 5500 Shindler Dr, Jacksonville, FL 32222 | Preschool |
| Pinewood Christian Academy | 198 Knight Boxx Rd, Middleburg, FL 32068 | Preschool |
| Plantation Christian Academy | 6263 Roosevelt Boulevard, Jacksonville, FL 32244 | Preschool |
| Primrose School of Fleming Island | 2031 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| Rideout Elementary Preschool | 3065 Apalachicola Blvd, Middleburg, FL 32068 | Preschool |
| Shadowlawn Elementary School Preschool | 2945 County Road 218, Green Cove Springs, FL 32043 | Preschool |
| Sharing Tree Preschool at Fleming Island | 5900 Highway 17 South, Fleming Island, FL 32003 | Preschool |
| St. Giles Children's Center | 116 Foxridge Rd., Orange Park, FL 32065 | Preschool |
| St. Margaret's Children's House | 6865 Pine Avenue, Fleming Island, FL 32003 | Preschool |
| Sugar N Spice | 3211 Moody Avenue, Orange Park, FL 32065 | Preschool |
| Sunshine Academy of Orange Park | 782 Foxridge Center Dr., Orange Park, FL 32065 | Preschool |
| Tender Hearts Child Care | 29 Cinnamon Street, Middleburg, FL 32068 | Center |
| The Children's Corner | 1720 Smith Street, Orange Park, FL 32073 | Preschool |
| The Goddard School of Fleming Island | 2280 Village Square Parkway, Fleming Island, FL 32003 | Preschool |
| Tynes Elementary Preschool | 1550 Tynes Blvd, Middleburg, FL 32068 | Preschool |
| W E Cherry Elementary School Preschool | 420 Edison Drive, Orange Park, FL 32073 | Preschool |
| Wee Care DayCare & Preschool, Inc | 4717 County Road 218, Middleburg, FL 32068 | Preschool |
| Wilkinson Elementary School Preschool | 4965 County Road 218, Middleburg, FL 32068 | Preschool |
| Wonderworks OPUMC | 2036 Reed Street, Orange Park, FL 32073 | Preschool |
| World of Wonders Academy | 101 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| YMCA Pryme Time at Argyle Elementary | 2625 Spencer Plantation Blvd, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Coppergate Elementary | 2250 County Road 209 North, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Drs. Inlet Elementary | 2634 State Road 220, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Fleming Island Elementary | 4425 Lake Shore Drive, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Grove Park Elementary | 1643 Miller Street, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at JL Wilkinson | 4965 County Highway 218, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Lake Asbury Elementary | 2901 Sandridge Road, Green Cove Springs, FL 32043 | Center |
| YMCA Pryme Time at Lakeside Elementary | 2752 Moody Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Middleburg Elementary | 3958 Main Street, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Montclair Elementary | 2398 Moody Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Oakleaf Village Elementary | 410 Oakleaf Village Parkway, Orange Park, FL 32065 | Center |
| YMCA Pryme Time at Orange Park Elementary | 1401 Plainfield Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Paterson Elementary | 5400 Pine Avenue, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Plantation Oaks Elementary | 4150 Plantation Oaks Blvd, Orange Park, FL 32065 | Center |
| YMCA Pryme Time at Rideout Elementary | 3065 Apalachicola Boulevard, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Ridgeview Elementary | 421 Jefferson Ave., Orange Park, FL 32065 | Center |