| Childtime of Jacksonville | 8500 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Childtime of Jacksonville | 9950 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Childtime of Orange Park | 1510 Business Center Drive, Fleming Island, FL 32003 | Preschool |
| Eagle Harbor Parkway KinderCare | 1735 Eagle Harbor Pkwy, Fleming Island, FL 32003 | Preschool |
| Fruit Cove KinderCare | 2590 Race Track Road, Saint Johns, FL 32259 | Center |
| La Petite Academy of Jacksonville | 6601 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| La Petite Academy of Middleburg | 3150 County Road 220, Middleburg, FL 32068 | Preschool |
| La Petite Academy of Orange Park | 4321 US Highway 17, Fleming Island, FL 32003 | Preschool |
| Oakleaf KinderCare | 431 Drysdale, Orange Park, FL 32065 | Center |
| Old St Augustine Rd KinderCare | 4310 Barkoskie Rd, Jacksonville, FL 32258 | Preschool |
|
| Tutor Time of Jacksonville | 13734 St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| Tutor Time of Jacksonville | 8510 Watermill Boulevard South, Jacksonville, FL 32244 | Preschool |
| A Child's Place Montessori School (Mandarin) | 12601 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| Abbate's Day Care | 349 Blanding Boulevard, Orange Park, FL 32073 | Preschool |
| Advent Lutheran Preschool | 2156 Loch Rane Boulevard, Orange Park, FL 32073 | Preschool |
| Andromeda Preschool | 131 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| Angels Elite Academy | 3560 Marbon Road, Jacksonville, FL 32223 | Preschool |
| Annunciation Catholic School | 1610 Blanding Blvd., Middleburg, FL 32068 | Preschool |
| Apple Tree Academy Childcare | 1246 Park Avenue, Orange Park, FL 32073 | Center |
| Argyle Christian Preschool and Kindergarten | 6823 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Arora's Clubhouse | 330 Arora Boulevard, Orange Park, FL 32073 | Preschool |
| Asbury Learning Center | 2781 Henley Road, Green Cove Springs, FL 32043 | Preschool |
| Atcom Child Care Learning Center | 5512 Westconnett Blvd, Jacksonville, FL 32244 | Center |
| Backpack University | 400 College Drive, Middleburg, FL 32068 | Center |
| Bannerman Learning Center | 608 Mill Street, Green Cove Springs, FL 32043 | Preschool |
| Belmont Head Start | 15 Belmont Blvd, Orange Park, FL 32073 | Preschool |
| Blue Angels Child Development Center Learning | 2025 Hwy 16 West, Green Cove Springs, FL 32043 | Preschool |
| Blueprints for life Childcare Academy | 11367 Old St. Augustine Road, Jacksonville, FL 32257 | Preschool |
| Bright Scholars Learning Center | 3003 Claire Lane, Jacksonville, FL 32223 | Preschool |
| Bright Start School and Daycare | 217 Wells Road, Orange Park, FL 32073 | Center |
| Celebration Children's Development Center | 806 Oak Street, Green Cove Springs, FL 32043 | Center |
| Challenge Enterprises Lighthouse Learning | 550 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Childtime Learning Center | 2890 Moody Avenue, Orange Park, FL 32073 | Preschool |
| Citi Family Center Jacksonville | 14000 Citi Cards Way Building F, Jacksonville, FL 32258 | Preschool |
| Doctors Inlet Elementary Preschool | 2634 Cr 220, Middleburg, FL 32068 | Preschool |
| Episcopal Children's Services Early Learning Center | 2506 Blanding Boulevard, Middleburg, FL 32068 | Preschool |
| Episcopal Children's Services Early Learning Center | 1107 Martin Luther King Jr Boulevard, Green Cove Springs, FL 32043 | Preschool |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| First Christian Preschool | 11924 San Jose Blvd, Jacksonville, FL 32223 | Preschool |
| First Class Learning Center | 939 Blanding Blvd., Orange Park, FL 32065 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Fleming Island Elementary School Preschool | 4425 Lakeshore Drive, Fleming Island, FL 32003 | Preschool |
| Fox Meadows Chrisitian Daycare | 3227 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Fruit Cove Baptist Weekday Preschool | 501 State Road 13, Saint Johns, FL 32259 | Center |
| Gingerbread Learning Center | 30 Knight Boxx Road, Orange Park, FL 32065 | Preschool |
| Good Samaritan Learning Tree | 3813 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Good Shepherd Lutheran Church Precious Lambs Preschool | 6551 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Grace Episcopal Dayschool | 156 Kingsley Avenue, Orange Park, FL 32073 | Center |
| Grove Park Elementary School Preschool | 1643 Miller Street, Orange Park, FL 32073 | Preschool |
| Hickory Grove Early Learning Center | 310 S. Oakridge Ave., Green Cove Springs, FL 32043 | Preschool |
| ISIS LITTLE HOMESCHOOL LLC | 8500 BLACKBERRY LANE EAST, Jacksonville, FL 32244 | Preschool |
| Island View Baptist Child Care Center | 900 Park Avenue, Orange Park, FL 32073 | Preschool |
| Ivy League Academy | 2245 Plantation Center Drive Suite 60, Fleming Island, FL 32003 | Preschool |
| Ivy League Academy at Oak Leaf | 7629 Old Middleburg Road South, Jacksonville, FL 32222 | Preschool |
| Jacksonville Adventist Academy | 4298 Livingston Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Jewish Center | 3662 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Julington Creek KinderCare - CLOSED | 200 N Ridgecrest Ln, Saint Johns, FL 32259 | Preschool |
| Just 4 Kids Learning Academy | 4371 Losco Rd., Jacksonville, FL 32257 | Preschool |
| Just Kids Learning Center | 120 Parkwood Drive East, Orange Park, FL 32073 | Preschool |
| Kampus Kids | 2300 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Keystone Heights Elementary School Preschool | 335 S. Pecan Street, Orange Park, FL 32065 | Preschool |
| Kids Expanding the Kingdom Christian Preschool | 3749 Crownpoint Road, Jacksonville, FL 32257 | Preschool |
| Kids World of Florida | 2121 Constitution Drive, Orange Park, FL 32073 | Preschool |
| KidsFirst Learning Center | 2645 Blanding Blvd, Middleburg, FL 32068 | Preschool |
| Kimbari Learning Academy | 3651 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Kirkwood Presbyterian Center of Early Learning | 8701 Argyle Forest Blvd, Jacksonville, FL 32244 | Center |
| Lake Asbury Elementary Preschool | 2901 Sandridge Rd, Green Cove Springs, FL 32043 | Preschool |
| Learning Ladders Child Development Center | 11270 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| Little Britches | 10829 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Little Cardinal's Nest | 1205 Roberts Road, Saint Johns, FL 32259 | Center |
| Little Paws Learning Center | 466 Madison Avenue, Orange Park, FL 32065 | Preschool |
| Little People's Learning Center | 5095 Lakeshore Drive West, Fleming Island, FL 32003 | Preschool |
| Living Waters Preschool | 2189 State Road 13, Saint Johns, FL 32259 | Preschool |
| Mandarin Childhood Learning Center | 4228 Hood Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Prep School | 3968 Sunbeam Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Presbyterian Preschool | 11844 Mandarin Rd., Jacksonville, FL 32223 | Preschool |
| Merry Pats Preschool | 1461 Fruit Cove Road South, Saint Johns, FL 32259 | Preschool |
| Mulberry Lane Childcare | 57 College Drive, Orange Park, FL 32065 | Preschool |
| Naval Air Station Jacksonville Child Development Center | 705 Child Street, Jacksonville, FL 32212 | Preschool |
| O.P.K. South of Middleburg | 3425 Habitat Drive, Middleburg, FL 32068 | Center |
| O2B Kids 7 | 1821 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| O2B Kids 7 | 1821 Town Center Blvd. Suite 101, Fleming Island, FL 32003 | Preschool |
| Oakleaf Preschool & Childcare | 415 Meldrum Lane, Orange Park, FL 32065 | Preschool |
| Oakleaf Village Elementary Preschool | 410 Oakleaf Village Parkway, Orange Park, FL 32073 | Preschool |
| Ollie's Day Care | 158 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park Kindergarten | 3050 Moody Rd, Orange Park, FL 32065 | Preschool |
| Orange Park Presbyterian Church Preschool | 1905 Park Avenue, Orange Park, FL 32073 | Preschool |
| Orange Park Preschool and Learning Center | 99 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park's Little Miracles | 1981 Wells Rd, Orange Park, FL 32073 | Preschool |
| Ortega Hills Preschool | 5029 Greenway Drive, Jacksonville, FL 32244 | Preschool |
| Pinewood Christian Academy | 198 Knight Boxx Rd, Middleburg, FL 32068 | Preschool |
| Plantation Christian Academy | 6263 Roosevelt Boulevard, Jacksonville, FL 32244 | Preschool |
| Precious Promises Preschool | 10365 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Primrose School of Fleming Island | 2031 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| Primrose School of Julington Creek | 480 State Road 13 North, Saint Johns, FL 32259 | Preschool |
| Promise Land Preschool | 3990 Loretto Road, Jacksonville, FL 32223 | Center |
| Rideout Elementary Preschool | 3065 Apalachicola Blvd, Middleburg, FL 32068 | Preschool |
| Ronelli's Academy | 12078 Old St. Augustine Road, Jacksonville, FL 32258 | Center |
| Saint Joseph Catholic School | 11600 Old St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| San Juan Del Rio Catholic School | 1714 State Road 13, Saint Johns, FL 32259 | Preschool |