| Ivy League Academy at Oak Leaf | 7629 Old Middleburg Road South, Jacksonville, FL 32222 | Preschool |
| JUST KIDS Learning Center | 6247 Ricker Road, Jacksonville, FL 32244 | Preschool |
| Jacksonville Adventist Academy | 4298 Livingston Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Jewish Center | 3662 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Urban League Head Start | 3811 St Augustine Road, Jacksonville, FL 32207 | Preschool |
| Jacksonville Urban League Normandy Heads | 6803 Arques Road, Jacksonville, FL 32205 | Preschool |
| Jewish Community Alliance | 8505 San Jose Boulevard, Jacksonville, FL 32217 | Center |
| Juliette's Day Care | 6939 Como Road, Jacksonville, FL 32210 | Preschool |
| Julington Creek KinderCare - CLOSED | 200 N Ridgecrest Ln, Saint Johns, FL 32259 | Preschool |
| Just 4 Kids Learning Academy | 4371 Losco Rd., Jacksonville, FL 32257 | Preschool |
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| Just Kids Learning Center | 120 Parkwood Drive East, Orange Park, FL 32073 | Preschool |
| Kampus Kids | 2300 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Keystone Heights Elementary School Preschool | 335 S. Pecan Street, Orange Park, FL 32065 | Preschool |
| Kharacter Kid Christian Academy | 1523 Chaffee Road South, Jacksonville, FL 32221 | Preschool |
| Kias Little Angels | 5870-2&3 San Juan Boulevard, Jacksonville, FL 32210 | Preschool |
| Kid's Academy USA | 1842 Blanding Blvd Ste 3, Jacksonville, FL 32210 | Preschool |
| Kiddieville Learning Center | 6820 Daughtry Blvd. South, Jacksonville, FL 32210 | Center |
| Kids Expanding the Kingdom Christian Preschool | 3749 Crownpoint Road, Jacksonville, FL 32257 | Preschool |
| Kids Park | 4274 Hershel St., Jacksonville, FL 32210 | Preschool |
| Kids United Childcare & Learning Center | 7037-1 Phillips Highway Suite 1, Jacksonville, FL 32216 | Preschool |
| Kids World Academy Westside Jacksonville | 4932 Blanding Blvd, Jacksonville FL, Jacksonville, FL 32210 | Center |
| Kids World of Florida | 2121 Constitution Drive, Orange Park, FL 32073 | Preschool |
| Kids World of Florida | 2597 Oak Street, Jacksonville, FL 32204 | Preschool |
| Kids World of Florida | 4040 Confederate Point Road, Jacksonville, FL 32210 | Preschool |
| Kidz Klub Developmental Learning Center | 4804 Blanding Boulevard, Jacksonville, FL 32210 | Preschool |
| Kimbari Learning Academy | 3651 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Kingdom Kare Learning Center | 5827 Seaboard Avenue, Jacksonville, FL 32244 | Center |
| Kirkwood Presbyterian Center of Early Learning | 8701 Argyle Forest Blvd, Jacksonville, FL 32244 | Center |
| Lakewood Presbyterian Church Preschool | 2001 University Boulevard West, Jacksonville, FL 32217 | Preschool |
| Lakewood United Methodist Preschool | 6133 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
| Lane Avenue Child Development Center | 1650 Lane Avenue, Jacksonville, FL 32210 | Preschool |
| Lang's Little Lambs Preschool | 5310 Lenox Ave Unit #26, Jacksonville, FL 32205 | Preschool |
| Learning Ladders Child Development Center | 11270 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| Learning Time Academy | 5301 Ricker Road, Jacksonville, FL 32210 | Preschool |
| Lil' Nuggets Daycare Center | 7054 Wilson Boulevard, Jacksonville, FL 32210 | Preschool |
| Little Britches | 10829 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Little Friends | 4001 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Little Paws Learning Center | 466 Madison Avenue, Orange Park, FL 32065 | Preschool |
| Little People Child Care Center II | 5868 Lenox Avenue, Jacksonville, FL 32205 | Preschool |
| Little People's Learning Center | 5095 Lakeshore Drive West, Fleming Island, FL 32003 | Preschool |
| Little Royalty Learning Center | 4833 Plymouth Street, Jacksonville, FL 32205 | Center |
| Little Saints Child Care Center | 2179 Emerson Street, Jacksonville, FL 32207 | Preschool |
| Little Tots Day Care | 6019 Lynnwood Avenue, Jacksonville, FL 32210 | Preschool |
| Living Waters Preschool | 2189 State Road 13, Saint Johns, FL 32259 | Preschool |
| Lollipop Kids Early Learning Center | 1233 Lane Avenue South #39, Jacksonville, FL 32205 | Preschool |
| Mandarin Childhood Learning Center | 4228 Hood Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Prep School | 3968 Sunbeam Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Presbyterian Preschool | 11844 Mandarin Rd., Jacksonville, FL 32223 | Preschool |
| Merry Pats Preschool | 1461 Fruit Cove Road South, Saint Johns, FL 32259 | Preschool |
| Merry Pop-Ins Child Care Center | 3935 St. Johns Avenue, Jacksonville, FL 32205 | Preschool |
| Methodist Children's VIllage | 7915 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Mulberry Lane Childcare | 57 College Drive, Orange Park, FL 32065 | Preschool |
| Murray Hill Baptist Child Care | 4300 Post Street, Jacksonville, FL 32205 | Preschool |
| Nanny's Little Angels Learning Center | 8531 Normandy Blvd., Jacksonville, FL 32221 | Center |
| Naval Air Station Jacksonville Child Development Center | 705 Child Street, Jacksonville, FL 32212 | Preschool |
| New Dimension Christian Early Education Center | 5505 Normandy Bvld, Jacksonville, FL 32205 | Preschool |
| Normandy Village Elementary Preschool | 8257 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Normandy Village Learning Academy | 8119 Herlong Road, Jacksonville, FL 32210 | Preschool |
| O.P.K. South of Middleburg | 3425 Habitat Drive, Middleburg, FL 32068 | Center |
| O2B Kids 7 | 1821 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| O2B Kids 7 | 1821 Town Center Blvd. Suite 101, Fleming Island, FL 32003 | Preschool |
| Oak Crest Child Care | 5900 Ricker Rd, Jacksonville, FL 32244 | Preschool |
| Oak Hill Early Learning Center | 4151 Old Middleburg Road N., Jacksonville, FL 32210 | Preschool |
| Oak Hill Elementary Preschool | 6910 Daughtry Blvd South, Jacksonville, FL 32210 | Preschool |
| Oakleaf Preschool & Childcare | 415 Meldrum Lane, Orange Park, FL 32065 | Preschool |
| Oakleaf Village Elementary Preschool | 410 Oakleaf Village Parkway, Orange Park, FL 32073 | Preschool |
| Ollie's Day Care | 158 College Drive, Orange Park, FL 32065 | Preschool |
| On The Right Track Child Development Center | 3325 Plymouth Street 14, Jacksonville, FL 32205 | Preschool |
| Orange Park Kindergarten | 3050 Moody Rd, Orange Park, FL 32065 | Preschool |
| Orange Park Presbyterian Church Preschool | 1905 Park Avenue, Orange Park, FL 32073 | Preschool |
| Orange Park Preschool and Learning Center | 99 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park's Little Miracles | 1981 Wells Rd, Orange Park, FL 32073 | Preschool |
| Ortega Hills Preschool | 5029 Greenway Drive, Jacksonville, FL 32244 | Preschool |
| Ortega Play School | 4807 Roosevelt Boulevard, Jacksonville, FL 32210 | Preschool |
| Page of Learning | 5809 Southpoint Parkway, Jacksonville, FL 32216 | Preschool |
| Parks Place Daycare and Learning Center | 5500 Shindler Dr, Jacksonville, FL 32222 | Preschool |
| Peas In A Pod Learning Center | 9652 Hood Road, Jacksonville, FL 32257 | Preschool |
| Pinedale Elementary Preschool | 4229 Edison Avenue, Jacksonville, FL 32254 | Preschool |
| Pinewood Christian Academy | 198 Knight Boxx Rd, Middleburg, FL 32068 | Preschool |
| Plantation Christian Academy | 6263 Roosevelt Boulevard, Jacksonville, FL 32244 | Preschool |
| Pokey's Angelic Christian Children's Academy | 3863 St. Augustine Road, Jacksonville, FL 32207 | Preschool |
| Precious Promises Preschool | 10365 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Primrose School of Fleming Island | 2031 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| Primrose School of Julington Creek | 480 State Road 13 North, Saint Johns, FL 32259 | Preschool |
| Progressive Learning Center | 1855 Hamilton Street, Jacksonville, FL 32210 | Preschool |
| Promise Land Preschool | 3990 Loretto Road, Jacksonville, FL 32223 | Center |
| Punkin Place Child Development of Jax | 6641 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Rideout Elementary Preschool | 3065 Apalachicola Blvd, Middleburg, FL 32068 | Preschool |
| Rising Stars of Tomorrow Preschool | 6304 Hyde Park Haven, Jacksonville, FL 32210 | Preschool |
| Ronelli's Academy | 12078 Old St. Augustine Road, Jacksonville, FL 32258 | Center |
| Ruth Upson Elementary Preschool | 1090 Dancy Street, Jacksonville, FL 32205 | Preschool |
| Sacred Heart Catholic School | 5752 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Saint Marks Ark Child Development Center | 3976 Hendricks Ave, Jacksonville, FL 32207 | Preschool |
| Saint Paul's Catholic School | 2609 Park Street, Jacksonville, FL 32204 | Preschool |
| San Carlos Learning Center | 2045 Gilmore Street, Jacksonville, FL 32204 | Preschool |
| San Jose Catholic School | 3619 Toledo Road, Jacksonville, FL 32217 | Preschool |
| San Jose Elementary Preschool | 5805 St. Agustine Road, Jacksonville, FL 32207 | Preschool |
| San Juan Del Rio Catholic School | 1714 State Road 13, Saint Johns, FL 32259 | Preschool |
| Seventh Generation Academy | 10679 Old St. Augustine Rd, Jacksonville, FL 32257 | Preschool |
| Sharing Tree Preschool at Fleming Island | 5900 Highway 17 South, Fleming Island, FL 32003 | Preschool |