| Childtime of Jacksonville | 8500 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Childtime of Jacksonville | 9950 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Childtime of Orange Park | 1510 Business Center Drive, Fleming Island, FL 32003 | Preschool |
| Eagle Harbor Parkway KinderCare | 1735 Eagle Harbor Pkwy, Fleming Island, FL 32003 | Preschool |
| Fruit Cove KinderCare | 2590 Race Track Road, Saint Johns, FL 32259 | Center |
| La Petite Academy of Jacksonville | 9220 Golfside Drive, Jacksonville, FL 32256 | Preschool |
| La Petite Academy of Jacksonville | 6601 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| La Petite Academy of Middleburg | 3150 County Road 220, Middleburg, FL 32068 | Preschool |
| La Petite Academy of Orange Park | 4321 US Highway 17, Fleming Island, FL 32003 | Preschool |
| Oakleaf KinderCare | 431 Drysdale, Orange Park, FL 32065 | Center |
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| Old St Augustine Rd KinderCare | 4310 Barkoskie Rd, Jacksonville, FL 32258 | Preschool |
| Tutor Time of Jacksonville | 13734 St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| Tutor Time of Jacksonville | 8510 Watermill Boulevard South, Jacksonville, FL 32244 | Preschool |
| 1st Journey Learning Center | 1759 Parkwood St, Jacksonville, FL 32207 | Preschool |
| A Bright Beginning Child Care | 8386 Baymeadow Road Suite 100, Jacksonville, FL 32256 | Preschool |
| A Bright Beginning Childcare | 8625 Old Kings Road South Suite ..., Jacksonville, FL 32217 | Center |
| A Child's Place Montessori School | 3718 Salisbury Road, Jacksonville, FL 32216 | Preschool |
| A Child's Place Montessori School (Mandarin) | 12601 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| A Gift From God Child Development Center | 2104 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| A Little Preschool House | 4504 Sunderland Road, Jacksonville, FL 32210 | Preschool |
| A Precious Gift From God Child Development Center | 1785 Cassat Ave, Jacksonville, FL 32210 | Center |
| ABC Learning Center of Jacksonville | 8341 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Abbate's Day Care | 349 Blanding Boulevard, Orange Park, FL 32073 | Preschool |
| Abundantly Loved Early Preschool Academy | 5154 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Advent Lutheran Preschool | 2156 Loch Rane Boulevard, Orange Park, FL 32073 | Preschool |
| All Saints Early Learning & Community Center | 4171 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Alpha Omega Learning Center | 2004 Hyde Park Road, Jacksonville, FL 32210 | Preschool |
| Andromeda Preschool | 131 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| Angels Elite Academy | 3560 Marbon Road, Jacksonville, FL 32223 | Preschool |
| Annunciation Catholic School | 1610 Blanding Blvd., Middleburg, FL 32068 | Preschool |
| Apple Tree Academy Childcare | 1246 Park Avenue, Orange Park, FL 32073 | Center |
| Argyle Christian Preschool and Kindergarten | 6823 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Arora's Clubhouse | 330 Arora Boulevard, Orange Park, FL 32073 | Preschool |
| Atcom Child Care Learning Center | 5512 Westconnett Blvd, Jacksonville, FL 32244 | Center |
| Avondale United Methodist Child's Day Out | 1651 Talbot Avenue, Jacksonville, FL 32205 | Preschool |
| Backpack University | 400 College Drive, Middleburg, FL 32068 | Center |
| Bank of America Child Development Center | 9000 Southside Boulevard Buildin ..., Jacksonville, FL 32256 | Preschool |
| Baymeadows Baptist Day School | 4826 Baymeadows Road, Jacksonville, FL 32217 | Preschool |
| Baymeadows KinderCare - CLOSED | 8401 Baymeadows Way, Jacksonville, FL 32256 | Preschool |
| Belmont Head Start | 15 Belmont Blvd, Orange Park, FL 32073 | Preschool |
| Blueprints for life Childcare Academy | 11367 Old St. Augustine Road, Jacksonville, FL 32257 | Preschool |
| Bosman Family Child Care Center | 5150 Timuquanna Road Unit 1, Jacksonville, FL 32210 | Preschool |
| Bright Future Learning Center | 8062 Old Kings Road South, Jacksonville, FL 32217 | Preschool |
| Bright Scholars Learning Center | 3003 Claire Lane, Jacksonville, FL 32223 | Preschool |
| Bright Start School and Daycare | 217 Wells Road, Orange Park, FL 32073 | Center |
| Builders of the Faith Christian Daycare | 7540 103rd Street #216, Jacksonville, FL 32210 | Preschool |
| Burrell's Child Care Center | 4162 Spring Park Circle, Jacksonville, FL 32207 | Preschool |
| Cedar Hills Baptist Christian School | 4200 Jammes Road, Jacksonville, FL 32210 | Center |
| Challenge Enterprises Lighthouse Learning | 550 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Chappell Child Development Center | 8400 Baycenter Road, Jacksonville, FL 32256 | Preschool |
| Chappell at Florida State College at Jacksonville | 3939 Roosevelt Boulevard, Jacksonville, FL 32205 | Preschool |
| Children's Gifting Circle Child Development Center | 1561 Cassat Ave., Jacksonville, FL 32210 | Center |
| Childrenville Learning Center | 5576 Timuquana Road #101, Jacksonville, FL 32210 | Preschool |
| Childtime Learning Center | 2890 Moody Avenue, Orange Park, FL 32073 | Preschool |
| Chosen Ones Learning Center | 4953 Firestone Road, Jacksonville, FL 32210 | Preschool |
| Chosen Vessels Christian Academy Preschool | 4545-3 Shirley Avenue, Jacksonville, FL 32210 | Preschool |
| Citi Family Center Jacksonville | 14000 Citi Cards Way Building F, Jacksonville, FL 32258 | Preschool |
| Community Academy of Jacksonville | 2655 Community Road, Jacksonville, FL 32207 | Preschool |
| Cornerstone Christian School VPK | 4000 Spring Park Rd, Jacksonville, FL 32207 | Preschool |
| Cornerstone Kids Day School | 5285 Shad Road Suite 103, Jacksonville, FL 32257 | Preschool |
| D C Academy | 2642 Firestone Road, Jacksonville, FL 32210 | Center |
| Dawn's Daycare and Learning Center | 1775 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| Deermeadows Preschool | 9780 Baymeadows Road, Jacksonville, FL 32256 | Preschool |
| Doctors Inlet Elementary Preschool | 2634 Cr 220, Middleburg, FL 32068 | Preschool |
| ECS Early Learning at Exchange South | 9143 Phillips Highway Suite 500, Jacksonville, FL 32256 | Preschool |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| First Christian Preschool | 11924 San Jose Blvd, Jacksonville, FL 32223 | Preschool |
| First Class Learning Center | 939 Blanding Blvd., Orange Park, FL 32065 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Fleming Island Elementary School Preschool | 4425 Lakeshore Drive, Fleming Island, FL 32003 | Preschool |
| Flower Garden Early Education | 3602 Park Street, Jacksonville, FL 32205 | Center |
| Fox Meadows Chrisitian Daycare | 3227 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Fruit Cove Baptist Weekday Preschool | 501 State Road 13, Saint Johns, FL 32259 | Center |
| Gingerbread House Learning Center | 6005 Chester Ave., Jacksonville, FL 32217 | Preschool |
| Gingerbread Learning Center | 30 Knight Boxx Road, Orange Park, FL 32065 | Preschool |
| Good Shepherd Lutheran Church Precious Lambs Preschool | 6551 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Grace Episcopal Dayschool | 156 Kingsley Avenue, Orange Park, FL 32073 | Center |
| Graham Bears Childcare | 5151 Colonial Ave., Jacksonville, FL 32210 | Preschool |
| Grove Park Elementary School Preschool | 1643 Miller Street, Orange Park, FL 32073 | Preschool |
| Growing Minds Development Center | 1181 Cassat Avenue, Jacksonville, FL 32205 | Center |
| Happy Hands Christian Child Care | 2017 Lane Avenue South, Jacksonville, FL 32210 | Preschool |
| Heaven Sent Daycare | 2151 Lane Ave. Suite. 102 & ..., Jacksonville, FL 32210 | Center |
| Holy Family | 9800-3 Baymeadows Road, Jacksonville, FL 32256 | Preschool |
| Honey Bear's Daycare | 1903 Fouraker Road, Jacksonville, FL 32210 | Center |
| Honey Bears Pre School | 8026 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Hyde Park Elementary Preschool | 5300 Park Street, Jacksonville, FL 32205 | Preschool |
| ISIS LITTLE HOMESCHOOL LLC | 8500 BLACKBERRY LANE EAST, Jacksonville, FL 32244 | Preschool |
| Island View Baptist Child Care Center | 900 Park Avenue, Orange Park, FL 32073 | Preschool |
| It's A Small World | 6723 Golfview Street, Jacksonville, FL 32210 | Preschool |
| Ivy League Academy | 2245 Plantation Center Drive Suite 60, Fleming Island, FL 32003 | Preschool |
| Ivy League Academy at Oak Leaf | 7629 Old Middleburg Road South, Jacksonville, FL 32222 | Preschool |
| JUST KIDS Learning Center | 6247 Ricker Road, Jacksonville, FL 32244 | Preschool |
| Jacksonville Adventist Academy | 4298 Livingston Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Jewish Center | 3662 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Urban League Head Start | 3811 St Augustine Road, Jacksonville, FL 32207 | Preschool |
| Jewish Community Alliance | 8505 San Jose Boulevard, Jacksonville, FL 32217 | Center |
| Juliette's Day Care | 6939 Como Road, Jacksonville, FL 32210 | Preschool |
| Julington Creek KinderCare - CLOSED | 200 N Ridgecrest Ln, Saint Johns, FL 32259 | Preschool |
| Just 4 Kids Learning Academy | 4371 Losco Rd., Jacksonville, FL 32257 | Preschool |
| Just Kids Learning Center | 120 Parkwood Drive East, Orange Park, FL 32073 | Preschool |