| 1st Journey Learning Center | 1759 Parkwood St, Jacksonville, FL 32207 | Preschool |
| A Child's Choice Development Center | 7044 Beach Boulevard, Jacksonville, FL 32216 | Center |
| A Gift From God Child Development Center | 2104 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| A Little Preschool House | 4504 Sunderland Road, Jacksonville, FL 32210 | Preschool |
| A New Beginning Child Care | 2528 W. Beaver St., Jacksonville, FL 32254 | Preschool |
| A New Beginning Early Learning Center | 5639 Ansley St, Jacksonville, FL 32211 | Center |
| A New Creation Learning Center | 4924 Moncrief Road West, Jacksonville, FL 32209 | Center |
| A Precious Gift From God Child Development Center | 1785 Cassat Ave, Jacksonville, FL 32210 | Center |
| A.R.T. Learning Center | 6180 Fort Caroline Road Suite 2, Jacksonville, FL 32277 | Preschool |
| ABC Christian Academy and Preschool | 2360 Kings Road, Jacksonville, FL 32209 | Preschool |
|
| ABC Learning Center of Jacksonville | 8341 Herlong Road, Jacksonville, FL 32210 | Preschool |
| ABCD Center | 735 S. Mcduff Ave, Jacksonville, FL 32205 | Center |
| Abundantly Loved Early Preschool Academy | 5154 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Academic Scholars Christian Academy | 2209 University Boulevard North, Jacksonville, FL 32211 | Preschool |
| Academie de Montessori | 1216 Lasalle Street, Jacksonville, FL 32207 | Preschool |
| Aladdan Christian Learning Center | 3839 Division Street, Jacksonville, FL 32209 | Preschool |
| Aldridge Day Care Center | 2721 Penton Street, Jacksonville, FL 32209 | Preschool |
| All About God's Children Academy | 3550 Westlake Avenue, Jacksonville, FL 32206 | Preschool |
| All About Kids | 4563 Pearl Street North, Jacksonville, FL 32206 | Preschool |
| All About Kids VPK Academy | 4204 Pearl Street North, Jacksonville, FL 32206 | Center |
| All Saints Early Learning & Community Center | 4171 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Alpha Omega Learning Center | 2004 Hyde Park Road, Jacksonville, FL 32210 | Preschool |
| Amazing Kids Learning Center | 3509 Kingston St., Jacksonville, FL 32254 | Preschool |
| Andrew Robinson Elementary Preschool | 101 West 12th Street, Jacksonville, FL 32206 | Preschool |
| Annie R. Morgan Elementary Preschool | 964 St. Clair Street, Jacksonville, FL 32254 | Preschool |
| Arlington Baptist Childcare Center | 6009 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Arlington Christian Academy | 5606 Windermere Drive, Jacksonville, FL 32211 | Preschool |
| Arlington Kindergarten of Jacksonville | 1210 Marcheck St., Jacksonville, FL 32211 | Preschool |
| As We Grow Preschool & Early Learning Center | 8927 Lem Turner Road, Jacksonville, FL 32208 | Preschool |
| Assumption Catholic School | 2431 Atlantic Boulevard, Jacksonville, FL 32207 | Preschool |
| Atcom Child Care Learning Center | 5512 Westconnett Blvd, Jacksonville, FL 32244 | Center |
| Audrey's Angels | 7803 Lueders Avenue, Jacksonville, FL 32208 | Preschool |
| Avondale United Methodist Child's Day Out | 1651 Talbot Avenue, Jacksonville, FL 32205 | Preschool |
| Bay Bay Kids Learning Center | 7047 Atlantic Boulevard Suite 2, Jacksonville, FL 32211 | Center |
| Beverly Hills Development Center | 3731 Rogers Avenue, Jacksonville, FL 32208 | Preschool |
| Bibleway Child Care Center | 3716 N. Pearl Street, Jacksonville, FL 32206 | Center |
| Biltmore Elementary Preschool | 2101 West Palm Avenue, Jacksonville, FL 32254 | Preschool |
| Blooming Blossoms Daycare Center | 1014 East 21st Street, Jacksonville, FL 32206 | Center |
| Bosman Family Child Care Center | 5150 Timuquanna Road Unit 1, Jacksonville, FL 32210 | Preschool |
| Bright Stars Early Learning Center | 7344 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Builders of the Faith Christian Daycare | 7540 103rd Street #216, Jacksonville, FL 32210 | Preschool |
| Burrell's Child Care Center | 4162 Spring Park Circle, Jacksonville, FL 32207 | Preschool |
| C.U.C.I.O.M Little Disciples | 2725 North Laura Street, Jacksonville, FL 32206 | Preschool |
| CHILDRENS FANTASY WORLD | 438 smith street, Jacksonville, FL 32204 | Center |
| Carter G. Woodson Elementary Preschool | 2334 Butler Avenue, Jacksonville, FL 32209 | Preschool |
| Cedar Hills Baptist Christian School | 4200 Jammes Road, Jacksonville, FL 32210 | Center |
| Chappell Corretta Scott King at FSCJ | 101 West State Street, Jacksonville, FL 32202 | Preschool |
| Chappell Premier Schools - Riverside | 484 Riverside Avenue, Jacksonville, FL 32202 | Preschool |
| Chappell at FCCJ North Campus | 4501 Capper Road, Jacksonville, FL 32218 | Preschool |
| Chappell at Florida State College at Jacksonville | 3939 Roosevelt Boulevard, Jacksonville, FL 32205 | Preschool |
| Chappell at Riverplace | 1301 Riverplace Boulevard, Jacksonville, FL 32207 | Preschool |
| Chatman's Day Care | 1614 Leonid Rd, Jacksonville, FL 32218 | Preschool |
| Chatman's Day Care & Learning Center | 3334 Moncrief Road, Jacksonville, FL 32209 | Center |
| Children of Liberty Child Care Center | 232 East 19th Street, Jacksonville, FL 32206 | Center |
| Children of Tomorrow Learning Center | 2950 Leonid Road, Jacksonville, FL 32218 | Preschool |
| Children's Choice Learning Center | 6046 Harvin Road, Jacksonville, FL 32216 | Preschool |
| Children's Fantasy World Pre-School | 438 Smith Street, Jacksonville, FL 32204 | Preschool |
| Children's Gifting Circle Child Development Center | 1561 Cassat Ave., Jacksonville, FL 32210 | Center |
| Childrenville Learning Center | 5576 Timuquana Road #101, Jacksonville, FL 32210 | Preschool |
| Chosen Ones Learning Center | 4953 Firestone Road, Jacksonville, FL 32210 | Preschool |
| Chosen Vessels Christian Academy Preschool | 4545-3 Shirley Avenue, Jacksonville, FL 32210 | Preschool |
| Christ The King Catholic School | 6822 Larkin Road, Jacksonville, FL 32211 | Preschool |
| Christ the King Child Care | 720 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Cinderella Child Development Center | 44 East 27th Street, Jacksonville, FL 32206 | Preschool |
| Cindy C's Day Nursery & Child Care | 9044 Crystal Springs Road, Jacksonville, FL 32221 | Preschool |
| Clayton Preschool Academy | 1419 East 15th Street, Jacksonville, FL 32206 | Preschool |
| Community Academy of Jacksonville | 2655 Community Road, Jacksonville, FL 32207 | Preschool |
| Community Connections of Jacksonville | 325 East Duval Street, Jacksonville, FL 32202 | Preschool |
| Cornerstone Christian School VPK | 4000 Spring Park Rd, Jacksonville, FL 32207 | Preschool |
| Country Bumpkins Child Care | 10805 Old Gainesville Road, Jacksonville, FL 32221 | Preschool |
| Crane's Land of Enchantment | 7442 Gainesville Ave, Jacksonville, FL 32208 | Preschool |
| Creative Little Hands | 6027 Kennerly Road, Jacksonville, FL 32216 | Preschool |
| Creative World Learning Center | 10852 Lem Turner Rd, Jacksonville, FL 32218 | Preschool |
| Crysties New World Academy II | 2933 Leonid Road, Jacksonville, FL 32218 | Center |
| D C Academy | 2642 Firestone Road, Jacksonville, FL 32210 | Center |
| D L C Nurse & Learn | 4101 College Street, Jacksonville, FL 32205 | Preschool |
| Dawn's Daycare and Learning Center | 1775 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| Dayspring Christian Academy and Preschool | 5654 Dunn Avenue, Jacksonville, FL 32218 | Preschool |
| Daystar Daycare & Learning Academy | 8061 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Debbie's Day Care | 325 Chestnut Drive, Jacksonville, FL 32208 | Center |
| Diamond In A Rough Learning Center | 142 Lane Avenue South, Jacksonville, FL 32254 | Preschool |
| Different Faces | 6221 Merrill Road, Jacksonville, FL 32277 | Center |
| Eagle Academy | 8985 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Early Learning Center of Arlington | 930 Cesery Boulevard, Jacksonville, FL 32211 | Center |
| Eastport Preschool & Kindergarten | 1322 Eastport Road, Jacksonville, FL 32218 | Center |
| Emerald City Learning Center | 5411 Soutel Drive, Jacksonville, FL 32219 | Preschool |
| Episcopal Children's Services Early Learning Center | 1070 West 18th Street, Jacksonville, FL 32209 | Preschool |
| Every Child Can Learn Child Development Center | 8301 West Beaver Street, Jacksonville, FL 32220 | Preschool |
| Exceptional Day Care & The Arts | 5111 North Pearl Street, Jacksonville, FL 32208 | Preschool |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| Fields Christian Academy | 6620 Arlington Expressway, Jacksonville, FL 32211 | Center |
| First Baptist Junior Academy | 125 West Ashley St, Jacksonville, FL 32202 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| First Coast Young Stars Learning Center | 3911 North Main Street, Jacksonville, FL 32206 | Preschool |
| First New Zion Christian Academy | 4819 Soutel Drive, Jacksonville, FL 32208 | Preschool |
| Flower Garden Early Education | 3602 Park Street, Jacksonville, FL 32205 | Center |
| Footprints In The Sand Child Development Center | 1303 North Edgewood Avenue, Jacksonville, FL 32254 | Preschool |
| For Your Child Only # 1 | 2183 W 15th Street, Jacksonville, FL 32209 | Center |
| For Your Child Only Day Care #4 | 2135 Kings Road, Jacksonville, FL 32209 | Preschool |
| For Your Child Only Victory Land | 6101 Avenue B, Jacksonville, FL 32209 | Preschool |