| Hunt Club Montessori School | 502 Hunt Club Boulevard, Apopka, FL 32703 | Preschool |
| Hyer Street Skool | 407 N Hyer Ave, Orlando, FL 32803 | Preschool |
| IEC Learning Center | 2500 West Oakridge Road, Orlando, FL 32809 | Preschool |
| Ingenuity Preschool | 2505 E. Semoran Blvd, Apopka, FL 32703 | Preschool |
| Ingram's Academy | 7856 Forest City Road, Orlando, FL 32810 | Preschool |
| Irene's Christian Academy | 3403 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Itty Bitty Learning Center | 4513 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Ivey Lane Elementary School Preschool | 209 N. Silverton St, Orlando, FL 32811 | Preschool |
| Jardin D'enfants Pre-school | 623 W Lancaster Rd, Orlando, FL 32809 | Preschool |
| Jewish Community Center Childcare | 851 North Maitland Avenue, Maitland, FL 32751 | Preschool |
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| Journey Early Learning Center | 9580 Curry Ford Road, Orlando, FL 32825 | Preschool |
| Just Babies & Kids | 4509-4505-4501 North Pine Hills ..., Orlando, FL 32808 | Center |
| Kaley Elementary School Preschool | 1600 Kaley Street, Orlando, FL 32806 | Preschool |
| Kid Avenue Learning Center | 121 North Pine Hills Road, Orlando, FL 32811 | Preschool |
| Kid City USA - Douglas Ave | 841 Douglas Avenue, Altamonte Springs, FL 32714 | Preschool |
| Kid City USA - Longwood | 935 Wekiva Springs Rd., Longwood, FL 32779 | Center |
| Kid City USA Winter Park | 3650 Howell Branch Road, Winter Park, FL 32792 | Preschool |
| Kid E Place | 26 West Steele Street, Orlando, FL 32804 | Preschool |
| Kid's Kingdom Daycare and Christian Academy | 1342 Bruton Blvd, Orlando, FL 32805 | Preschool |
| Kid's Prep School | 823 S Semoran Blvd, Winter Park, FL 32792 | Preschool |
| Kiddie Haven Day Nursery | 1851 Goodrich Ave, Winter Park, FL 32789 | Preschool |
| Kids & Company | 4709 Curry Ford Road, Orlando, FL 32812 | Preschool |
| Kids & Company Raeford Road | 2447 Raeford Rd, Orlando, FL 32812 | Preschool |
| Kids Above and Beyond Learning Center | 6782 North Orange Blossom Trail, Orlando, FL 32810 | Preschool |
| Kids Ark Development Center | 1238 Bunnell Road, Altamonte Springs, FL 32714 | Preschool |
| Kids Carousel of Central Florida | 1527 Dodd Rd, Winter Park, FL 32792 | Preschool |
| Kids First Steps | 191 Normandy Road, Casselberry, FL 32707 | Preschool |
| Kids Kingdom Christian Academy | 1342 Bruton Blvd., Orlando, FL 32805 | Preschool |
| Kids R Kids 2 Oviedo | 315 Alafaya Woods Boulevard, Oviedo, FL 32765 | Preschool |
| Kids Resort II | 9367 Trevarthon Road, Orlando, FL 32817 | Preschool |
| Kids Time Kiddie Care | 197 South Cottage Hill Road, Orlando, FL 32805 | Center |
| Kidtown USA | 1460 Tuskawilla Road, Winter Springs, FL 32708 | Preschool |
| Kidz Choice | 4429 Cypress Street, Orlando, FL 32811 | Preschool |
| Kidz R 4 Uz | 6700 Silver Star Rd Suite 4, Orlando, FL 32818 | Preschool |
| Kidz at Work | 2035 West Washington Street, Orlando, FL 32805 | Preschool |
| Killarney Elementary School Preschool | 2401 Wellington Blvd, Winter Park, FL 32789 | Preschool |
| Kinder Kampus | 640 E Sr 434, Winter Springs, FL 32708 | Preschool |
| KinderCare at Hunt Club - CLOSED | 625 Executive Park Court, Apopka, FL 32703 | Preschool |
| Kinderoo Preschool & Child Care | 389 Spring Oak Blvd, Altamonte Springs, FL 32714 | Preschool |
| King of Kings Lutheran Preschool | 1101 N Wymore Rd, Maitland, FL 32751 | Preschool |
| Kingdom Kids Academy | 2047 Prospect Avenue, Orlando, FL 32814 | Preschool |
| Kingsway Christian Academy | 4161 N Powers Dr, Orlando, FL 32818 | Center |
| La Petite Academy # 173 Oviedo - CLOSED | 204 Alexandria Boulevard, Oviedo, FL 32765 | Preschool |
| La Shundra's Tots Child Care & Development Center | 140 West Street Suite B, Maitland, FL 32751 | Preschool |
| Ladybird Academy Lake Underhill | 10955 Lake Underhill Rd., Orlando, FL 32825 | Preschool |
| Ladybird Academy at Winter Springs | 282 Sr 434, Winter Springs, FL 32708 | Preschool |
| Lake Forrest Preparatory School | 866 Lake Howell Road, Maitland, FL 32751 | Center |
| Lake Gem Elementary School Preschool | 4801 Bloodhound St, Orlando, FL 32818 | Preschool |
| Lake Orienta Preschool | 612 Newport Avenue, Altamonte Springs, FL 32701 | Preschool |
| Lake Underhill Montessori | 523 Chickasaw Trail, Orlando, FL 32825 | Preschool |
| Lake Weston Elementary School Preschool | 5500 Milian Dr, Orlando, FL 32810 | Preschool |
| Lakeville Elementary School Preschool | 2015 Lakeville Road, Apopka, FL 32703 | Preschool |
| Lancaster Elementary School Preschool | 6700 Sheryl Ann Drive, Orlando, FL 32809 | Preschool |
| Landmark Community Church | 1001 West Sr 434, Winter Springs, FL 32708 | Preschool |
| Lawton Elementary Vpk - Seminole County | 151 Graham Avenue, Oviedo, FL 32765 | Preschool |
| Learning Development Center | 1175 Benjamin Avenue, Winter Park, FL 32789 | Preschool |
| Life Changers Academy | 4900 Donovan Street, Orlando, FL 32808 | Preschool |
| Light Years Ahead Child Care Center | 398 Douglas Ave., Altamonte Springs, FL 32714 | Preschool |
| Lighthouse Christian Academy Early Development | 4390 Grand Avenue, Casselberry, FL 32730 | Center |
| Lil Rascals Child Care Center | 2701 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Lila Mitchell Head Start | 5151 Raleigh Street, Orlando, FL 32806 | Preschool |
| Little Angels Child Care | 5700 Silver Star Road, Orlando, FL 32808 | Preschool |
| Little Angels Christian Academy | 1340 West Michigan Street, Orlando, FL 32805 | Preschool |
| Little Angels Preschool | 10012 Portale Ave, Orlando, FL 32825 | Preschool |
| Little Anointed Ones Learning Center | 2612 Pioneer Road, Orlando, FL 32808 | Center |
| Little Citizens Child Care | 1112 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Little Creations Child Care | 2740 W. Washington St, Orlando, FL 32805 | Preschool |
| Little Flower Academy | 980 South Eagle Circle, Casselberry, FL 32707 | Center |
| Little Lords and Ladies | 570 N Alafaya Trail Suite 101-c, Orlando, FL 32828 | Preschool |
| Little Miracles Academy | 900 Plymouth Avenue, Orlando, FL 32805 | Center |
| Little People Learning Center | 1033 W Michigan St, Orlando, FL 32805 | Preschool |
| Little People's College | 615 Pine Hills Road, Orlando, FL 32808 | Preschool |
| Little People's Reading Club | 241 W Kennedy Blvd, Maitland, FL 32751 | Preschool |
| Little Rae's Christian Center | 3099 Orange Center Boulevard, Orlando, FL 32805 | Preschool |
| Little River Elementary School Preschool | 100 Caswell Dr, Orlando, FL 32825 | Preschool |
| Little Stars Child Care | 4501 Goddard Ave, Orlando, FL 32804 | Preschool |
| Little Steps | 26 Willow Drive, Orlando, FL 32807 | Preschool |
| Lovell Elementary School Preschool | 815 Roger Williams Rd, Apopka, FL 32703 | Preschool |
| Loving Earth Preschool & Development Center | 813 N. Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| Loving Hands Childcare Learning Center | 216 North Ivey Lane, Orlando, FL 32811 | Preschool |
| Magnolia Academy and Child Care Center | 4607 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Mai Little Starz Learning Academy | 6837 & 6841 W Colonial Dr, Orlando, FL 32818 | Preschool |
| Maitland Community School | 1300 W Maitland Blvd, Maitland, FL 32751 | Preschool |
| Maitland Montessori School | 236 North Swoope Ave, Maitland, FL 32751 | Preschool |
| Maitland Montessori School | 236 North Swoope Avenue, Maitland, FL 32751 | Center |
| Mama's Pride & Joy Preschool and Child Care | 933 South Ivey Lane, Orlando, FL 32811 | Preschool |
| Margie's Non-Stop Kiddie Kare | 903 Plymouth Avenue, Orlando, FL 32805 | Preschool |
| Markham Woods Christian Academy | 1675 Dixon Road, Longwood, FL 32779 | Preschool |
| Mary's Little Lambs Day Care Center | 1122 N Powers Dr, Orlando, FL 32818 | Preschool |
| Matrix Child Care and Learning Center | 2138 W. Colonial Drive, Orlando, FL 32804 | Preschool |
| Merriday Montessori School | 2600 E. Jackson Street, Orlando, FL 32803 | Preschool |
| Methodist School For Early Education | 125 N Interlachen Ave, Winter Park, FL 32789 | Center |
| Metro Kids | 220 E Wildmere Ave, Longwood, FL 32750 | Preschool |
| Millenia Elementary Preschool | 5301 Cypress Creek Blvd, Orlando, FL 32811 | Preschool |
| Mills Child Development Center | 5200 West South Street, Orlando, FL 32811 | Center |
| Mission Road Church Day Care | 151 Mission Road, Oviedo, FL 32765 | Center |
| Mollie Ray Elementary School Preschool | 2000 Beecher St., Orlando, FL 32808 | Preschool |
| Montessori Bilingual Academy | 61 South Dean Road, Orlando, FL 32825 | Preschool |
| Montessori Kids Academy | 1932 N. Goldenrod Road, Orlando, FL 32807 | Preschool |
| Ms B's Angels Child Care | 2514 W Colonial Dr, Orlando, FL 32804 | Preschool |