| Hillsdale KinderCare | 601 Hillsdale Dr, Charlottesville, VA 22901 | Center |
| Riverbend KinderCare | 201 Riverbend Dr, Charlottesville, VA 22911 | Center |
| ABC Preschool | 925 E. Market Street, Charlottesville, VA 22902 | Preschool |
| ACAC Preschool | 200 Four Seasons Drive, Charlottesville, VA 22901 | Preschool |
| Apple Tree Academies at Pantops | 201 Riverbend Drive, Charlottesville, VA 22911 | Preschool |
| Apple Tree Academies at Woodlands | 601 Hillsdale Drive, Charlottesville, VA 22901 | Preschool |
| Atlantic Coast Athletic Club ACAC Afterschool Kidz Club | 200 Four Seasons Drive, Charlottesville, VA 22901 | Center |
| Barrett Early Learning Center | 410 Ridge Street, Charlottesville, VA 22902 | Preschool |
| Beau Pre Kindergarten | 1570 Beau Pre Lane, Charlottesville, VA 22901 | Center |
| Boar's Head Sports Club Child Care | 200 Wellington Drive, Charlottesville, VA 22903 | Center |
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| Bounce-n-Play of Charlottesville | 127 Seminole Court, Charlottesville, VA 22901 | Center |
| Bright Beginnings Preschool - Mill Creek | 1221 Stony Ridge Road, Charlottesville, VA 22902 | Preschool |
| Bright Beginnings Preschool at Forest Lakes | 3401 Worth Crossing, Charlottesville, VA 22911 | Preschool |
| Chancellor Street Preschool Cooperative | 1700 University Avenue, (St. Paul's Memorial Church), Charlottesville, VA 22903 | Preschool |
| Charlottesville Day School | 320 10th Street, NE, Charlottesville, VA 22902 | Preschool |
| Charlottesville Waldorf School | 722 E. Rio Road, Charlottesville, VA 22901 | Preschool |
| Charlottesville Waldorf School | 120 Waldorf School Road, Charlottesville, VA 22901 | Preschool |
| Children Come First Child Care Center | 2320 Hunter's Way, Charlottesville, VA 22911 | Center |
| First Steps Infant Development Center | 1100 East Jefferson Street, Charlottesville, VA 22902 | Center |
| Foundations Child Development Center | 3375 Berkmar Drive, Charlottesville, VA 22901 | Preschool |
| Four Seasons Learning Center | 254 Lakeview Drive, Charlottesville, VA 22901 | Preschool |
| Hilltop Day Care Center | 909 Landonia Circle, Charlottesville, VA 22901 | Preschool |
| JABA Shining Star Pre-School | 674 Hillsdale Drive, Suite 9, Charlottesville, VA 22901 | Preschool |
| Lake Co-Op Preschool | 110 Sweetbriar Park Road, Locust Grove, VA 22508 | Center |
| MACAA - Clark Head Start | 1000 Belmont Avenue, Charlottesville, VA 22902 | Preschool |
| MACAA Baker-Butler Head Start | 2740 Proffit Road, Charlottesville, VA 22911 | Preschool |
| MACAA Jordan, Twyman & Ferguson Head Start Centers | 1025 Park Street, Charlottesville, VA 22901 | Preschool |
| Mighty Minds, Little Hands International Preschool | 2117 Angus Road, Charlottesville, VA 22901 | Preschool |
| Molly Michie Preschool | 717 Rugby Road, Charlottesville, VA 22903 | Preschool |
| Montessori School of Charlottesville | 631 Cutler Lane, Charlottesville, VA 22901 | Preschool |
| Montessori School of Charlottesville | 1602 Gordon Avenue, Charlottesville, VA 22903 | Preschool |
| Northern Neck Head Start - Lancaster | 36 Primary Circle, Lancaster, VA 22503 | Preschool |
| Old Dominion Day School | 74 Barclay Place Court, Charlottesville, VA 22901 | Preschool |
| Our Neighborhood Child Development Center | 2110 Ivy Road, Charlottesville, VA 22903 | Center |
| Peabody School | 1232 Stoney Ridge Road, Charlottesville, VA 22902 | Preschool |
| Piedmont Family YMCA ASPIRE Afterschool Program | 1525 Stony Point Road, Charlottesville, VA 22911 | Center |
| Piedmont Family YMCA Child Care Center | 442 Westfield Road, Charlottesville, VA 22901 | Center |
| Piedmont YMCA - Park's Edge Afterschool Program | 181 Whitewood Road, Charlottesville, VA 22901 | Center |
| St. Anne's - Belfield After School Enrichment Program | 2132 Ivy Road, Charlottesville, VA 22903 | Preschool |
| St. Anne's - Belfield School | 799 Faulconer Drive, Charlottesville, VA 22903 | Preschool |
| The Covenant School - The Covenant Club | 1000 Birdwood Road, Charlottesville, VA 22903 | Preschool |
| The Frost Montessori School of Albemarle | 1553 Delphi Drive, Charlottesville, VA 22911 | Preschool |
| The International School of Charlottesville | 830 Monticello Avenue, Charlottesville, VA 22902 | Preschool |
| University Montessori School | 1034 Reservoir Road, Charlottesville, VA 22903 | Preschool |
| University of Virginia Child Dev Center - CLOSED | 408 Earhart Street, Charlottesville, VA 22903 | Center |
| Wee Care Daycare 4 Kids | 1550 Seminole Trail, Charlottesville, VA 22906 | Center |