| Lakewood United Methodist Preschool | 6133 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
| Lane Avenue Child Development Center | 1650 Lane Avenue, Jacksonville, FL 32210 | Preschool |
| Lang's Little Lambs Preschool | 5310 Lenox Ave Unit #26, Jacksonville, FL 32205 | Preschool |
| Learning Time Academy | 5301 Ricker Road, Jacksonville, FL 32210 | Preschool |
| Leslie's Angels | 2114 Tilden Street, Jacksonville, FL 32206 | Center |
| Lil Frenche's Christian Learning Center | 5013 Broadway Avenue, Jacksonville, FL 32254 | Preschool |
| Lil' Nuggets Daycare Center | 7054 Wilson Boulevard, Jacksonville, FL 32210 | Preschool |
| Lil' Sprouts Learning Center | 8840-7 Atlantic Blvd., Jacksonville, FL 32211 | Preschool |
| Little Blessings Academy | 1822 West 30th Street, Jacksonville, FL 32209 | Preschool |
| Little Bundles of Joy Learning Center and Daycare | 101 West 48th Street Suite 11, Jacksonville, FL 32208 | Preschool |
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| Little Diamonds Big Heart Day Care | 1441 East 31st Street, Jacksonville, FL 32206 | Center |
| Little Disciples in Training Christian Childcare II | 3428 Winton Drive, Jacksonville, FL 32208 | Preschool |
| Little Dove Day Care & Learning Center | 7525 New Kings Road, Jacksonville, FL 32219 | Preschool |
| Little Friends | 4001 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Little Miracles Early Learning Academy | 101 - 8 West 48th Street, Jacksonville, FL 32208 | Preschool |
| Little People Child Care Center II | 5868 Lenox Avenue, Jacksonville, FL 32205 | Preschool |
| Little Royalty Learning Center | 4833 Plymouth Street, Jacksonville, FL 32205 | Center |
| Little Saints Child Care Center | 2179 Emerson Street, Jacksonville, FL 32207 | Preschool |
| Little Sarephims Daycare | 220 Mill Creek Road, Jacksonville, FL 32211 | Center |
| Little Temples Child Care Center | 2467 Faye Road Unit #13, Jacksonville, FL 32226 | Preschool |
| Little Temples Child Care Center II | 9100 Merrill Road Unit 15, Jacksonville, FL 32225 | Preschool |
| Little Tots Day Care | 6019 Lynnwood Avenue, Jacksonville, FL 32210 | Preschool |
| Little Wise Kids Early Learning Center | 6318 Arlington Road, Jacksonville, FL 32211 | Center |
| Little Wise Kids Early Learning Center II | 5703 Arlington Road, Jacksonville, FL 32211 | Center |
| Lollipop Kids Early Learning Center | 1233 Lane Avenue South #39, Jacksonville, FL 32205 | Preschool |
| Lone Star Learning Center | 8142 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Long Branch Elementary Preschool | 3723 Franklin Street, Jacksonville, FL 32206 | Preschool |
| Love-N-Care Day Care Center | 2948 Eden Street, Jacksonville, FL 32254 | Preschool |
| Lula's Kids | 1239 West Edgewood Avenue, Jacksonville, FL 32208 | Preschool |
| McCrary's Learning Center | 6620 Arlington Expressway, Jacksonville, FL 32211 | Preschool |
| Merry Pop-Ins Child Care Center | 3935 St. Johns Avenue, Jacksonville, FL 32205 | Preschool |
| Methodist Children's VIllage | 7915 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Mine Yours & Ours Child Care Learning Center | 7645 Merrill Road #107, Jacksonville, FL 32277 | Preschool |
| Murray Hill Baptist Child Care | 4300 Post Street, Jacksonville, FL 32205 | Preschool |
| My First Step Learning Center | 10658 Biscayne Boulevard, Jacksonville, FL 32218 | Preschool |
| My First Step Learning Center II | 1638 Leonid Road, Jacksonville, FL 32218 | Preschool |
| Nakesa's Learning Center | 727 N Mcduff Avenue, Jacksonville, FL 32254 | Preschool |
| Nanny's Little Angels Learning Center | 8531 Normandy Blvd., Jacksonville, FL 32221 | Center |
| Nanny's Northside Nursery | 11033 Biscayne Blvd., Jacksonville, FL 32218 | Preschool |
| New Beginning Day Care Center | 2804 West Edgewood Avenue, Jacksonville, FL 32209 | Preschool |
| New Dimension Christian Early Education Center | 5505 Normandy Bvld, Jacksonville, FL 32205 | Preschool |
| New Life In Christ Child Development Center | 6225 Norwood Avenue, Jacksonville, FL 32208 | Center |
| Normandy Village Elementary Preschool | 8257 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Normandy Village Learning Academy | 8119 Herlong Road, Jacksonville, FL 32210 | Preschool |
| North Shore Elementary Preschool | 5701 Silver Plaza, Jacksonville, FL 32208 | Preschool |
| Northern Lights Sunshine Academy | 6240 Fort Caroline Road #1-4, Jacksonville, FL 32277 | Preschool |
| Northside Center For Creative Learning | 3534 Winton Drive, Jacksonville, FL 32208 | Preschool |
| Northside Community Involvement | 4736 Avenue B, Jacksonville, FL 32209 | Preschool |
| Oak Hill Early Learning Center | 4151 Old Middleburg Road N., Jacksonville, FL 32210 | Preschool |
| Oak Hill Elementary Preschool | 6910 Daughtry Blvd South, Jacksonville, FL 32210 | Preschool |
| Oceanway Elementary Preschool | 12555 Gillespie Avenue, Jacksonville, FL 32218 | Preschool |
| Old Plank Road Baptist Church Daycare | 8964 Old Plank Rd, Jacksonville, FL 32220 | Center |
| On The Right Track Child Development Center | 3325 Plymouth Street 14, Jacksonville, FL 32205 | Preschool |
| Once Upon A Time Development Center | 1129 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Ortega Play School | 4807 Roosevelt Boulevard, Jacksonville, FL 32210 | Preschool |
| Pam's Homelike Child Care and Learning Center | 3509 Soutel Drive, Jacksonville, FL 32208 | Preschool |
| Parkwood Weekday Early Education Center | 7900 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Parson's Little Scholars Creative Child Care | 6505 Fort Caroline Road, Jacksonville, FL 32277 | Preschool |
| Parsons Christian Academy | 5705 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Pearl Academy | 1680 Dunn Avenue Bldg. #5, Jacksonville, FL 32218 | Preschool |
| Pickett Elementary Preschool | 6305 Old Kings Road North, Jacksonville, FL 32254 | Preschool |
| Pinedale Elementary Preschool | 4229 Edison Avenue, Jacksonville, FL 32254 | Preschool |
| Playhouse of Fun & Knowledge II | 5045 Soutel Dr. #70, Jacksonville, FL 32208 | Preschool |
| Pokey's Angelic Christian Children's Academy | 3863 St. Augustine Road, Jacksonville, FL 32207 | Preschool |
| Pooh + Friends Child Care Development Center | 6029 Bennett Road, Jacksonville, FL 32216 | Preschool |
| Possibilities & Success Academy | 1157 Golfair Blvd, Jacksonville, FL 32209 | Center |
| Power Challengers Ministries | 4316 Barnes Road, Jacksonville, FL 32207 | Center |
| Praise Is What We Do Early Learning | 1225 West 29th Street, Jacksonville, FL 32209 | Preschool |
| Precious Angels Child Care & Learning Center | 1144 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Precious Hearts 24 Hour Learning | 1803 W 45th Street, Jacksonville, FL 32209 | Preschool |
| Progressive Learning Center | 1855 Hamilton Street, Jacksonville, FL 32210 | Preschool |
| Punkin Place Child Development of Jax | 6641 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Rawls Kiddie Kastle | 9316 1st Avenue, Jacksonville, FL 32208 | Preschool |
| Rawls Kiddie Kastle II | 5786 Mcleod Avenue, Jacksonville, FL 32219 | Preschool |
| Resurrection Catholic School | 3406 Justina Road, Jacksonville, FL 32277 | Preschool |
| Reynolds Lane Elementary Preschool | 840 Reynolds Lane, Jacksonville, FL 32254 | Preschool |
| Rising Stars of Tomorrow Preschool | 6304 Hyde Park Haven, Jacksonville, FL 32210 | Preschool |
| River of Life Child Care Center | 4537 Emerson Street, Jacksonville, FL 32207 | Center |
| Rufus Payne Elementary Preschool | 6725 Hema Road, Jacksonville, FL 32209 | Preschool |
| Ruth Upson Elementary Preschool | 1090 Dancy Street, Jacksonville, FL 32205 | Preschool |
| Rutledge Pearson Elementary Preschool | 4346 Roanoke Boulevard, Jacksonville, FL 32208 | Preschool |
| S & J Learning Academy | 1420 Oakhurst St, Jacksonville, FL 32208 | Center |
| S. A. Hull Elementary Preschool | 7528 Hull Street, Jacksonville, FL 32219 | Preschool |
| S. A. Hull Kindergarten | 4711 Avenue B, Jacksonville, FL 32209 | Preschool |
| S. P. Livingston Elementary Preschool | 1128 Barber Street, Jacksonville, FL 32209 | Preschool |
| Sacred Heart Catholic School | 5752 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Saint Claire Evans Academy | 5443 Moncrief Road, Jacksonville, FL 32209 | Preschool |
| Saint Marks Ark Child Development Center | 3976 Hendricks Ave, Jacksonville, FL 32207 | Preschool |
| Saint Patrick's Catholic School | 1429 Broward Road, Jacksonville, FL 32218 | Preschool |
| Saint Paul's Catholic School | 2609 Park Street, Jacksonville, FL 32204 | Preschool |
| Saint Pius School | 1470 W. 13th St., Jacksonville, FL 32209 | Preschool |
| Sallye B. Mathis Elementary Preschool | 3501 Winton Drive, Jacksonville, FL 32208 | Preschool |
| San Carlos Learning Center | 2045 Gilmore Street, Jacksonville, FL 32204 | Preschool |
| San Jose Elementary Preschool | 5805 St. Agustine Road, Jacksonville, FL 32207 | Preschool |
| Sensational Kids Learning Academy | 6043 Terry Road, Jacksonville, FL 32216 | Preschool |
| Small Beginnings Learning Center | 3533 Commonwealth Avenue, Jacksonville, FL 32254 | Center |
| Smart Start Learning Center | 1112 Fountain Road, Jacksonville, FL 32205 | Preschool |
| Soaring High Learning Center | 4218 Blanding Bvd, Jacksonville, FL 32210 | Preschool |
| South Jacksonville Presbyterian Preschool | 2137 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Southside United Methodist Church Preschool | 3120 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |