| Lake Mary KinderCare - CLOSED | 910 W Lake Mary Blvd, Sanford, FL 32773 | Preschool |
| Lake Underhill Montessori | 523 Chickasaw Trail, Orlando, FL 32825 | Preschool |
| Lake Underhill Montessori School | 1537 S. Alafaya Trail, Orlando, FL 32828 | Center |
| Landmark Community Church | 1001 West Sr 434, Winter Springs, FL 32708 | Preschool |
| Lawton Elementary Vpk - Seminole County | 151 Graham Avenue, Oviedo, FL 32765 | Preschool |
| Life Song Church Mothers Morning out Program | 2800 South Alafaya Trail, Orlando, FL 32828 | Center |
| Lighthouse Christian Academy Early Development | 4390 Grand Avenue, Casselberry, FL 32730 | Center |
| Little Angels Preschool | 10012 Portale Ave, Orlando, FL 32825 | Preschool |
| Little Flower Academy | 980 South Eagle Circle, Casselberry, FL 32707 | Center |
| Little Lords and Ladies | 570 N Alafaya Trail Suite 101-c, Orlando, FL 32828 | Preschool |
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| Little River Elementary School Preschool | 100 Caswell Dr, Orlando, FL 32825 | Preschool |
| Little Steps | 26 Willow Drive, Orlando, FL 32807 | Preschool |
| Loving Earth Preschool & Development Center | 813 N. Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| Maitland Montessori School | 236 North Swoope Ave, Maitland, FL 32751 | Preschool |
| Maitland Montessori School | 236 North Swoope Avenue, Maitland, FL 32751 | Center |
| Meadows Academy | 5779 North Ronald Reagan Boule ..., Sanford, FL 32773 | Preschool |
| Methodist School For Early Education | 125 N Interlachen Ave, Winter Park, FL 32789 | Center |
| Metro Kids | 220 E Wildmere Ave, Longwood, FL 32750 | Preschool |
| Mission Road Church Day Care | 151 Mission Road, Oviedo, FL 32765 | Center |
| Monarch Children's Academy | 13825 Townsend Drive, Orlando, FL 32828 | Preschool |
| Montessori Bilingual Academy | 61 South Dean Road, Orlando, FL 32825 | Preschool |
| Montessori Kids Academy | 1932 N. Goldenrod Road, Orlando, FL 32807 | Preschool |
| Mothercare Preschool & Child Care II | 2921 South Orlando Drive Suite 130, Sanford, FL 32773 | Preschool |
| Neighborhood Kids Academy | 222 Neighborhood Market Road ..., Orlando, FL 32825 | Preschool |
| New Beginnings Learning Academy - CLOSED | 10376 E. Colonial Drive, Orlando, FL 32817 | Preschool |
| New Covenant Preschool | 800 Tuskawilla Road, Oviedo, FL 32765 | Preschool |
| Noah's Ark Christian Preschool | 891 Sr 434 E, Longwood, FL 32750 | Preschool |
| Northland Early Learning Center | 530 Dog Track Rd., Longwood, FL 32750 | Center |
| Oak Arbor Chistian School Preschool and Child Care | 1902 Longwood Lake Mary Road, Longwood, FL 32750 | Center |
| Oviedo Academy Preschool | 824 Executive Drive, Oviedo, FL 32765 | Preschool |
| Oviedo Montessori School | 261 South Central Avenue, Oviedo, FL 32765 | Preschool |
| Page Private School of Orlando | 10250 University Boulevard, Orlando, FL 32817 | Center |
| Park Avenue Child Care of Sandford | 2700 S Park Ave, Sanford, FL 32773 | Preschool |
| Pee Wee Ranch | 210 S Wayman St, Longwood, FL 32750 | Preschool |
| Pee Wee Ranch Child Care II | 304 S Wayman Street, Longwood, FL 32750 | Center |
| Primary Learning Academy | 2042 N Forsyth Rd, Orlando, FL 32807 | Preschool |
| Primrose School at Avalon Park | 13461 Tanja King Boulevard, Orlando, FL 32828 | Preschool |
| Primrose School of Oviedo | 1933 West County Road 419, Oviedo, FL 32766 | Center |
| Redeemer Lutheran Learning Center | 3377 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Rising Star Child Care | 1455 South Highway 17-92, Longwood, FL 32750 | Preschool |
| Rollins College Child Development Center | 1000 Holt Ave Box 2760, Winter Park, FL 32789 | Center |
| Royal Academy Preschool | 295 Oxford Road, Casselberry, FL 32730 | Preschool |
| Small Wonders Academy of Longwood | 221 West State Road 434, Longwood, FL 32750 | Preschool |
| Smiles R Us Child Care Center | 8414 E Colonial Dr, Orlando, FL 32817 | Preschool |
| South Semionole Christian Church Preschool | 300 West State Road 434, Oviedo, FL 32765 | Preschool |
| St Mary Magdalen Child Center | 710 Spring Lake Road, Altamonte Springs, FL 32701 | Preschool |
| St Stephen Weekday School | 8601 Lake Underhill Rd, Orlando, FL 32825 | Preschool |
| St. Luke's Lutheran School | 2025 West State Road 426, Oviedo, FL 32765 | Preschool |
| St. Richard's Preschool | 1720 Howell Branch Road, Winter Park, FL 32789 | Center |
| StarChild Academy at Waterford | 12800 Waterford Lakes Parkway, Orlando, FL 32828 | Center |
| Starchild Academy Oviedo | 961 Eastbridge Drive, Oviedo, FL 32765 | Center |
| Sterling Park Elementary Vpk | 905 South Eagle Circle, Casselberry, FL 32707 | Preschool |
| Sts Peter & Paul Early Learning | 5300 Old Howell Branch Road, Winter Park, FL 32792 | Preschool |
| Sweetwater Kids Academy | 418 North Central Avenue, Oviedo, FL 32765 | Preschool |
| Teach Time Academy | 9300 University Blvd, Orlando, FL 32817 | Center |
| Terrific Kids Child Development Center | 7507 Aloma Avenue Suite #2, Winter Park, FL 32792 | Preschool |
| The Cambridge Prep School | 1015 Willa Springs Dr, Winter Springs, FL 32708 | Preschool |
| The Children's Place Learning Center | 2095 Dundee Dr, Winter Park, FL 32792 | Preschool |
| The Double R Private School | 725 Country School Trail, Oviedo, FL 32766 | Preschool |
| The Friends Academy | 53 S Dean Rd, Orlando, FL 32825 | Center |
| The Learning Tree | 1021 New York Avenue North, Winter Park, FL 32789 | Center |
| The Tree House Educational Center | 1851 S Goldenrod Rd, Orlando, FL 32822 | Preschool |
| Think and Play | 2751 Tuskawilla Road, Oviedo, FL 32765 | Preschool |
| Tiny Handz | 181 Oxford Road Suite 115, Casselberry, FL 32730 | Center |
| Top Kids Academy | 1912 North Dean Road, Orlando, FL 32817 | Preschool |
| Tot Spot | 01715 Lake Pickett Rd, Orlando, FL 32826 | Preschool |
| Tuskawilla Learning Center | 3925 Red Bug Lake Road, Casselberry, FL 32707 | Center |
| Tuskawilla Presbyterian School | 3600 W Sr 426, Oviedo, FL 32765 | Preschool |
| Twin Rivers Montessori | 1270 Lockwood Boulevard, Oviedo, FL 32765 | Center |
| UCF Creative School for Children | 4000 Central Florida Boulevard Bu ..., Orlando, FL 32816 | Preschool |
| Union Park Christian School | 10301 East Colonial Drive, Orlando, FL 32817 | Preschool |
| Union Park Elementary School Preschool | 1600 N. Dean Rd, Orlando, FL 32825 | Preschool |
| Union Park Preschool | 10689 East Colonial Drive, Orlando, FL 32817 | Preschool |
| United Cerebral Palsy - East Bailes Campus | 12702 Science Drive, Orlando, FL 32826 | Preschool |
| University Carillon Early Learning Center | 1385 Campus View Court, Oviedo, FL 32765 | Preschool |
| Vista Lakes Elementary Preschool | 6050 Champlain Dr, Orlando, FL 32826 | Preschool |
| Wee Learning Center | 2838 Percival Road, Orlando, FL 32826 | Center |
| Welbourne Avenue Nursery & Kindergarten | 450 Welbourne Ave, Winter Park, FL 32789 | Preschool |
| Wilford Woodruff Academy | 760- North Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| Willow Creek Preschool | 4725 East Lake Drive, Winter Springs, FL 32708 | Preschool |
| Winners Children Academy | 6816 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Winter Park Presbyterian Preschool | 400 South Lakemont Avenue, Winter Park, FL 32792 | Preschool |