| Alafaya Woods KinderCare | 300 Alafaya Woods Blvd, Oviedo, FL 32765 | Preschool |
| Aloma Methodist Early Childhood Learning Center | 3045 Aloma Avenue, Winter Park, Florida, Winter Park, FL 32792 | Preschool |
| Childtime of Oviedo | 1500 Swanson Drive, Oviedo, FL 32765 | Preschool |
| Childtime of Winter Park | 1001 South Semoran Boulevard, Winter Park, FL 32792 | Preschool |
| Goldenrod Road KinderCare | 4035 N Goldenrod Rd, Winter Park, FL 32792 | Preschool |
| La Petite Academy of Orlando | 401 Mark Twain Blvd, Orlando, FL 32828 | Preschool |
| Winter Springs KinderCare | 1024 Spring Villas Pt, Winter Springs, FL 32708 | Preschool |
| 4-C Headstart - Midway | 2255 Rightway Street, Sanford, FL 32771 | Center |
| 4-C Seminole Head Start - Lawton | 188 South Lake Jessup Road, Oviedo, FL 32765 | Center |
| 4C Head Start - North Street | 900 North Street, Longwood, FL 32750 | Preschool |
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| 4C Seminole Head Start - Hamilton | 1414 Celery Ave, Sanford, FL 32773 | Preschool |
| 4C Seminole Head Start Pinecrest | 405 W 27th Street, Sanford, FL 32773 | Center |
| A Growing Place Child Care | 18555 Old Cheney Hwy, Orlando, FL 32820 | Preschool |
| A Kid's Gym Fitness Center | 1495 Evans Street, Oviedo, FL 32765 | Preschool |
| A Tot's World II | 900 Silkwood Ct, Longwood, FL 32750 | Preschool |
| A Tot's World III | 480 West State Road 434, Winter Springs, FL 32708 | Preschool |
| All About Kidz of Oviedo | 387 West Broadway Street, Oviedo, FL 32765 | Preschool |
| All Saints School | 338 East Lyman Avenue, Winter Park, FL 32789 | Preschool |
| Aloma Elementary School Preschool | 2949 Scarlet Road, Winter Park, FL 32789 | Preschool |
| Aloma Kids Academy | 3416 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Aloma Kids of Orlando | 3416 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Altamonte Kiddie Cottage | 290 Maitland Ave, Altamonte Springs, FL 32701 | Preschool |
| Altamonte Learning Center | 1329 Altamonte Drive, Casselberry, FL 32707 | Preschool |
| Angel Sprouts Academy | 7651 Valencia College Lane, Orlando, FL 32807 | Preschool |
| Ascension Lutheran Early Childhood Program | 351 Ascension Drive, Casselberry, FL 32707 | Preschool |
| Audubon Elementary School Preschool | 1750 Common Way Rd, Orlando, FL 32814 | Preschool |
| Aunt Delores's Child Care | 1411 Melanie Drive, Orlando, FL 32825 | Preschool |
| Avalon Elementary School Preschool | 13500 Tanji King Blvd, Orlando, FL 32828 | Preschool |
| Azalea Park Baptist School | 5725 Dahlia Dr, Orlando, FL 32807 | Preschool |
| Azalea Park Elementary School Preschool | 1 Carol Avenue, Orlando, FL 32807 | Preschool |
| Bithlo Head Start | 18501 Washington Avenue, Orlando, FL 32820 | Preschool |
| Bright Horizons at Baldwin Park | 1700 Fire House Lane, Orlando, FL 32814 | Preschool |
| Brightfutures Academy | 1500 Shepard Road, Winter Springs, FL 32708 | Preschool |
| Brush Arbor Christian | 2304 North Goldenrod Road, Orlando, FL 32807 | Center |
| Camelot Elementary School Preschool | 14501 Waterford Chase Parkway, Orlando, FL 32828 | Preschool |
| Casselberry Elementary Vpk - Seminole Co | 1075 Crystal Bowl Circle, Casselberry, FL 32707 | Preschool |
| Castle Creek Elementary School Preschool | 1245 N. Avalon Park Blvd, Orlando, FL 32828 | Preschool |
| Center For Early Childhood Education | 341 North Orlando Avenue, Maitland, FL 32751 | Preschool |
| Central Florida Christian Preschool | 2218 Alafaya Trail, Oviedo, FL 32765 | Preschool |
| Cheney Elementary School Preschool | 2000 N. Forsyth Road, Orlando, FL 32807 | Preschool |
| Children 1st Preschool | 11648 McCulloch Rd, Orlando, FL 32817 | Center |
| Children's Prep Academy | 1883 S. Volusia Avenue, Oviedo, FL 32762 | Preschool |
| Choices In Learning Charter School Preschool | 893 E. State Road 434, Longwood, FL 32750 | Preschool |
| Chuck and Kerry's House | 234 Concord Drive, Casselberry, FL 32707 | Preschool |
| City Kidz Academy Preschool | 650 E Airport Blvd, Sanford, FL 32773 | Preschool |
| Community Alliance Preschool | 4815 E Lake Dr, Winter Springs, FL 32708 | Preschool |
| Community Christian Preschool | 4921 South U.s. Highway 17-92, Casselberry, FL 32707 | Preschool |
| Coqui Academy II | 919 Orange Avenue East, Longwood, FL 32750 | Preschool |
| Denton Johnson Head Start | 400 Ruffle Street, Maitland, FL 32751 | Preschool |
| Discovery Zone Preschool | 11333 Lake Underhill Rd, Orlando, FL 32825 | Preschool |
| Discovery Zone ll Preschool | 2876 S. Alafaya Trail, Orlando, FL 32828 | Preschool |
| Downey Christian Pre-School | 10201 E. Colonial Drive, Orlando, FL 32817 | Preschool |
| Early Childhood Academy | 1231 North Brassie Drive, Winter Springs, FL 32708 | Preschool |
| Early Childhood Center - First United Methodist | 263 King St, Oviedo, FL 32765 | Preschool |
| East Lake Elementary School Preschool | 3971 N Tanner Rd, Orlando, FL 32826 | Preschool |
| East Orange Head Start | 12050 East Colonial Drive, Orlando, FL 32826 | Preschool |
| Eastbrook Elementary Vpk - Seminole County | 5525 Tangerine Avenue, Winter Park, FL 32792 | Preschool |
| Eastland Christian School | 9000 Lakeunder Hill Road, Orlando, FL 32825 | Center |
| Einstein Academy | 1030 N. Ronald Reagan Blvd. #300a, Longwood, FL 32750 | Preschool |
| Einstein Academy - Campus 2 | 12046 Collegiate Way, Orlando, FL 32817 | Preschool |
| English Estates Pre-k | 299 Oxford Road, Casselberry, FL 32730 | Preschool |
| Evans Elementary Vpk Preschool | 100 Chapman Road, Oviedo, FL 32765 | Preschool |
| Faith Christian Academy Preschool | 2008 N Goldenrod Road, Orlando, FL 32807 | Center |
| First Congregational Church Preschool | 225 South Interlachen Avenue, Winter Park, FL 32789 | Preschool |
| First Friends | 789 N. Alafaya Tr, Orlando, FL 32828 | Preschool |
| First Steps Pre-School and Development Center | 12727 East Colonial Drive, Orlando, FL 32826 | Preschool |
| First Steps of Learning Camp Seminole | 390 Piney Ridge Road, Casselberry, FL 32707 | Preschool |
| First United Methodist Church Christian Child Care | 270 1st Street, Geneva, FL 32732 | Center |
| First Years Preschool and Kindergarten | 45 W Broadway St, Oviedo, FL 32765 | Center |
| Friends Preschool Academy | 327 Wilma Street, Longwood, FL 32750 | Preschool |
| Geneva Elementary Pre-k | 275 First Street, Geneva, FL 32732 | Preschool |
| Golden Bear Academy | 421 S Goldenrod Rd, Orlando, FL 32822 | Preschool |
| Good Shepherd Early Childhood Educational Center | 5902 Oleander Dr, Orlando, FL 32807 | Preschool |
| Growing Minds Educational Day Care Center | 11887 E. Colonial Dr., Orlando, FL 32826 | Center |
| Growing Together Academy | 843 Woodbury Rd, Orlando, FL 32828 | Preschool |
| Growing Tree Learning Center | 900 Dodd Road, Casselberry, FL 32707 | Preschool |
| Happyland Learning Academy | 1295 North Semoran Boulevard, Orlando, FL 32807 | Preschool |
| Hope Lutheran Preschool | 2600 North Dean Road, Orlando, FL 32817 | Preschool |
| Horizons Child Care Learning Center | 1089 N Goldenrod Rd, Orlando, FL 32807 | Preschool |
| Howell Branch Academy | 4605 Howell Branch Road, Winter Park, FL 32792 | Preschool |
| Jewish Community Center Childcare | 851 North Maitland Avenue, Maitland, FL 32751 | Preschool |
| Journey Early Learning Center | 9580 Curry Ford Road, Orlando, FL 32825 | Preschool |
| Kid City USA Winter Park | 3650 Howell Branch Road, Winter Park, FL 32792 | Preschool |
| Kid's Prep School | 823 S Semoran Blvd, Winter Park, FL 32792 | Preschool |
| Kiddie Haven Day Nursery | 1851 Goodrich Ave, Winter Park, FL 32789 | Preschool |
| Kids Carousel of Central Florida | 1527 Dodd Rd, Winter Park, FL 32792 | Preschool |
| Kids First Steps | 191 Normandy Road, Casselberry, FL 32707 | Preschool |
| Kids R Kids 2 Oviedo | 315 Alafaya Woods Boulevard, Oviedo, FL 32765 | Preschool |
| Kids R Kids 5 Waterford Lakes | 415 Woodbury Road, Orlando, FL 32828 | Preschool |
| Kids Resort II | 9367 Trevarthon Road, Orlando, FL 32817 | Preschool |
| Kids Together | 3029 Sanctuary Drive, Oviedo, FL 32766 | Preschool |
| Kidtown USA | 1460 Tuskawilla Road, Winter Springs, FL 32708 | Preschool |
| Kinder Kampus | 640 E Sr 434, Winter Springs, FL 32708 | Preschool |
| Kingdom Kids Academy | 2047 Prospect Avenue, Orlando, FL 32814 | Preschool |
| La Petite Academy # 173 Oviedo - CLOSED | 204 Alexandria Boulevard, Oviedo, FL 32765 | Preschool |
| Ladybird Academy Lake Underhill | 10955 Lake Underhill Rd., Orlando, FL 32825 | Preschool |
| Ladybird Academy at Avalon Park | 357 S. Avalon Park Road, Orlando, FL 32828 | Preschool |
| Ladybird Academy at Winter Springs | 282 Sr 434, Winter Springs, FL 32708 | Preschool |
| Lake Forrest Preparatory School | 866 Lake Howell Road, Maitland, FL 32751 | Center |
| Lake Mary Community Preschool | 560 Weldon Blvd, Lake Mary, FL 32746 | Preschool |