| La Petite Academy of Lakeland | 1940 Glendale Street, Lakeland, FL 33803 | Preschool |
| A Little Bit of Heaven Child Enrichment Center | 424 W Daughtery Road, Lakeland, FL 33809 | Center |
| A'Kelynn Angels Learning Center | 800 Havendale Blvd. N.w., Winter Haven, FL 33881 | Center |
| All Saints Academy | 5001 State Road 540 West, Winter Haven, FL 33880 | Center |
| Alpha Bright Children Learning Center | 438 Wabash Ave N, Lakeland, FL 33813 | Center |
| Alpha Kid Care | 2900 Sr 540a East, Lakeland, FL 33813 | Center |
| Alpha Kid Care at Highland City | 5335 9th Street Se, Highland City, FL 33846 | Center |
| Alpha Kids Learning Center | 1013 Lakeshore Dr, Lakeland, FL 33803 | Preschool |
| Beacon Hill Preschool | 801 West Beacon Road, Lakeland, FL 33803 | Preschool |
| Berkley Charter School | 5240 Berkley Road, Auburndale, FL 33823 | Preschool |
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| Bobbie's Busy Bee | 4105 Glen Road, Lakeland, FL 33810 | Preschool |
| Brenda's Little Helpers Christian Daycare | 1100 E Rose Street, Lakeland, FL 33801 | Center |
| Bright Imaginations | 6075 South Florida Ave., Lakeland, FL 33813 | Preschool |
| CT&S Annointed Learning Center II | 2302 Haywood Street, Lakeland, FL 33801 | Preschool |
| Calvary Child Care | 3800 Recker Highway, Winter Haven, FL 33880 | Center |
| Carousel Daycare | 929 Fairlane Drive, Lakeland, FL 33809 | Preschool |
| Children's Village Enrichment Center | 7405 Us Hwy 98 N, Lakeland, FL 33809 | Preschool |
| College Heights United Methodist Preschool | 942 South Boulevard, Lakeland, FL 33803 | Preschool |
| Color's R Us Child Development Center | 1533 Kennedy Blvd, Lakeland, FL 33810 | Preschool |
| Colors R Us Child Development Center | 1512 George Jenkins Blvd, Lakeland, FL 33815 | Center |
| Country Lane Child Enrichment Center | 105 West Daughtery Rd, Lakeland, FL 33809 | Center |
| Covenant Child Development Center | 210 E Poppell Drive, Lakeland, FL 33813 | Preschool |
| Creative Minds Academy II | 1802 Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Creative Minds Learning Academy | 3108 Atlantic Ave, Lakeland, FL 33801 | Center |
| Cuyler Learning Center | 613 Bennett Street, Auburndale, FL 33823 | Center |
| Cuyler Learning Preschool and School Age | 170 Dairy Road, Auburndale, FL 33823 | Center |
| Discovery Learning Academy | 3915 Old Highway 37, Lakeland, FL 33813 | Preschool |
| Early Childhood Academy George Jenkins | 6000 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| Early Childhood Learning Center | 1703 Skinner Street, Lakeland, FL 33801 | Preschool |
| Early Childhood Learning Center Airside | 3135 Airside Center Drive, Lakeland, FL 33811 | Preschool |
| Excel Christian Academy | 6505 Odom Road, Lakeland, FL 33809 | Center |
| First Academy Preschool Auburndale | 614 Main Street South, Auburndale, FL 33823 | Preschool |
| First Baptist Church of Highland City Preschool | 5410 Yarborough Lane, Lakeland, FL 33812 | Preschool |
| First Pentecostal Learning Center | 1440 Kathleen Road, Lakeland, FL 33805 | Center |
| First United Methodist Church Preschool | 72 Lake Morton Drive, Lakeland, FL 33801 | Preschool |
| Florida Southern College Preschool Lab | 111 Lake Hollingsworth Dr, Lakeland, FL 33801 | Center |
| Future Children Development Center | 2128 S Crystal Lake Dr, Lakeland, FL 33801 | Center |
| Futurecare Learning Center | 412 Old Winter Haven Road, Auburndale, FL 33823 | Preschool |
| Goal Seekers and Eagles Before and After School Program | 4730 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| God Little Helpers | 1641 E Memorial Blvd, Lakeland, FL 33801 | Center |
| Grace Lutheran Child Development Center | 745 South Ingraham Avenue, Lakeland, FL 33801 | Preschool |
| Greater St Paul Daycare | 1130 N Webster Ave, Lakeland, FL 33805 | Center |
| Hands-N-Hearts in Motion | 3330 Winterlake Road, Lakeland, FL 33803 | Preschool |
| Happy Days School | 926 South Tennessee Avenue, Lakeland, FL 33803 | Preschool |
| Haven Christian Academy | 2105 King Rd Sw, Winter Haven, FL 33880 | Center |
| Hazel Perkins Gray Child Development Center | 1620 W Parker St., Lakeland, FL 33815 | Center |
| Head of The Class Learning Center III | 1836 Kathleen Rd, Lakeland, FL 33805 | Preschool |
| Heavenly Kids Christian Academy | 2605 Sunshine Drive South, Lakeland, FL 33801 | Center |
| Henderson Children of Glory Childcare Center | 109 N 3rd St, Eagle Lake, FL 33839 | Center |
| Highlands Christian Academy | 4210 Lakeland Highlands Rd., Lakeland, FL 33813 | Center |
| Imagination Station of Polk County | 1200 34th St Nw, Winter Haven, FL 33881 | Preschool |
| JC's Early Learning Center | 2540 S Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Jeanni's Preschool and School Age | 905 Spirit Lake Rd, Winter Haven, FL 33880 | Center |
| Journey to Excellence CDC | 1315 W 14th St #134, Lakeland, FL 33805 | Preschool |
| Joyful Hearts Preschool a Ministry of Lakela | 1725 Parker Road, Lakeland, FL 33811 | Preschool |
| Karing 4 Kids Learning Center | 1010 Olive Street, Lakeland, FL 33815 | Center |
| Kathleen Baptist Child Enrichment Center | 3939 2nd Street Nw, Lakeland, FL 33810 | Center |
| Kathleen Preschool | 1100 Red Devil Way #c7, Lakeland, FL 33815 | Center |
| Kathleen Preschool Academy Preschool | 2600 Crutchfield Road #c8, Lakeland, FL 33805 | Preschool |
| Kids Town Preschool | 333 Griffin Avenue, Lakeland, FL 33801 | Preschool |
| Kids World Enrichment Center II | 932 Martin Luther King Junior Bou ..., Lakeland, FL 33815 | Center |
| King and Queen Learning Academy | 1102 N Ruth Avenue, Lakeland, FL 33805 | Center |
| Kountry Kids Preschool | 9960 Sr 33 N, Polk City, FL 33868 | Preschool |
| Lake Gibson Child Development Center | 6868 N Socrum Loop Rd, Lakeland, FL 33809 | Center |
| Lakeland Montessori Schoolhouse | 837 E. Parker Street, Lakeland, FL 33801 | Preschool |
| Learning Tree Child Development Center | 2995 Avenue G Nw, Winter Haven, FL 33880 | Preschool |
| Lighthouse Community Preschool | 215 East Magnolia St, Lakeland, FL 33801 | Center |
| Lit'le Bloodhound Pre-school | 222 Ariana Avenue, Auburndale, FL 33823 | Preschool |
| Little Achievers CDC | 2933 Duff Rd, Lakeland, FL 33810 | Preschool |
| Little Believers Daycare and Learning Center | 1700 George Jenkins Blvd, Lakeland, FL 33815 | Preschool |
| Little Footsteps of Lakeland | 6595 South Florida Avenue, Lakeland, FL 33813 | Preschool |
| Little Leapers Learning Center | 505 Peoples Lane, Lakeland, FL 33815 | Center |
| Little Shepherds Preschool | 175 Lake Hollingsworth Drive, Lakeland, FL 33801 | Center |
| Little World at Shepherd Road | 1333 Shepherd Rd, Lakeland, FL 33811 | Preschool |
| Lonnie's Little Professors Childcare Center | 905 E Parker St, Lakeland, FL 33801 | Center |
| McCloud's Childcare Center | 1741 E Gary Road, Lakeland, FL 33801 | Preschool |
| McGordon's Learning Center | 624 West 5th Street, Lakeland, FL 33805 | Preschool |
| Medulla Baptist Church Preschool Learning Center | 3930 Old Hwy 37, Lakeland, FL 33813 | Center |
| Miss Adele's Learning Center | 1804 Richmond Road, Lakeland, FL 33803 | Preschool |
| Montessori Preschool of Lakeland | 334 Doris Dr, Lakeland, FL 33813 | Preschool |
| Mrs Beasley's Learning Center | 923 Fairlane Drive, Lakeland, FL 33809 | Preschool |
| Mrs Heather's Lit'l Learning Center | 217 Palmetto St, Auburndale, FL 33823 | Center |
| Multi Level Tutoring | 824 Havendale Blvd Nw, Winter Haven, FL 33881 | Preschool |
| New Beginnings Preschool Learning Center | 5965 Sr 542 West, Winter Haven, FL 33880 | Preschool |
| Noah's Ark at United Methodist Temple | 2700 South Florida Avenue, Lakeland, FL 33803 | Preschool |
| Pathway of Lakeland | 1942 West Memorial Blvd, Lakeland, FL 33815 | Center |
| Precious Ones Learning Center | 234 Noxon Street, Auburndale, FL 33823 | Center |
| Preparing the Way Ministries Enrichment Center | 1712 West Chase Street, Lakeland, FL 33805 | Center |
| Presbyterian Church in the Highlands | 1010 Lake Miriam Dr, Lakeland, FL 33813 | Preschool |
| Progress Child Care Center | 1041 Breezewood Drive, Lakeland, FL 33809 | Preschool |
| Publix Child Development Center | 345 N Galloway Rd, Lakeland, FL 33815 | Center |
| Reach For Success | 768 31st Court Nw, Winter Haven, FL 33881 | Center |
| SACCA Boswell | 2820 K-ville Avenue, Auburndale, FL 33823 | Center |
| SACCA Inc Philip O'Brien Campus Kid Care | 1225 E Lime St, Lakeland, FL 33801 | Center |
| SACCA Jewett Kid Care | 2250 8th St Ne, Winter Haven, FL 33880 | Center |
| SACCA Lake Shipp Kid Care | 250 Camellia Dr, Winter Haven, FL 33880 | Center |
| SACCA Lincoln Academy | 1330 N Lincoln Avenue, Lakeland, FL 33805 | Center |
| SACCA Scott Lake Kid Care | 1140 E Sr 540a, Lakeland, FL 33813 | Center |
| Safe and Sound Child Care and Early Learning Center | 580 East Laurel Ave, Eagle Lake, FL 33839 | Preschool |
| Scott Lake Christian Preschool | 5811 Scott Lake Rd, Lakeland, FL 33813 | Center |