| Toni's Touch Pre-K | 1301 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Top Kids Academy | 1912 North Dean Road, Orlando, FL 32817 | Preschool |
| Tots in Toyland Learning Center | 11235 West Michigan Street, Orlando, FL 32805 | Preschool |
| Trinity Child Development Center | 420 & 424 Ruth Ln, Orlando, FL 32801 | Preschool |
| Trinity Christian Academy | 2113 East South Street, Orlando, FL 32803 | Center |
| True Deliverance Center Day Care | 29 South Ivey Lane, Orlando, FL 32811 | Center |
| Tuskawilla Learning Center | 3925 Red Bug Lake Road, Casselberry, FL 32707 | Center |
| Tuskawilla Presbyterian School | 3600 W Sr 426, Oviedo, FL 32765 | Preschool |
| UCF Creative School for Children | 4000 Central Florida Boulevard Bu ..., Orlando, FL 32816 | Preschool |
| Union Park Christian School | 10301 East Colonial Drive, Orlando, FL 32817 | Preschool |
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| Union Park Elementary School Preschool | 1600 N. Dean Rd, Orlando, FL 32825 | Preschool |
| Union Park Preschool | 10689 East Colonial Drive, Orlando, FL 32817 | Preschool |
| Unique Child Care Center | 1373 Bunnell Rd, Apopka, FL 32703 | Preschool |
| United Cerebral Palsy - East Bailes Campus | 12702 Science Drive, Orlando, FL 32826 | Preschool |
| United Cerebral Palsy - Pine Hills Child Development | 5800 Golf Club Parkway, Orlando, FL 32818 | Preschool |
| United Cerebral Palsy of Central FL - Hollow | 3305 S. Orange Avenue, Orlando, FL 32806 | Preschool |
| Universal Child Care Development | 1003 South Kirkman Road, Orlando, FL 32811 | Preschool |
| Ventura Elementary Preschool | 4400 Woodgate Blvd, Orlando, FL 32822 | Preschool |
| Washington Shores Elementary School Preschool | 944 West Lake Mann Drive, Orlando, FL 32805 | Preschool |
| We Love Kids | 636 N Wymore Road, Winter Park, FL 32789 | Preschool |
| Wee Kare Child Care Learning Academy | 5020 Silver Star Road, Orlando, FL 32808 | Preschool |
| Weekday School at First Presbyterian Church | 106 E. Church Street, Orlando, FL 32801 | Center |
| Welbourne Avenue Nursery & Kindergarten | 450 Welbourne Ave, Winter Park, FL 32789 | Preschool |
| Wesley Child Development Center | 42 E. Jackson Street, Orlando, FL 32801 | Preschool |
| West Oaks Elementary School Preschool | 905 Dorscher Rd, Orlando, FL 32818 | Preschool |
| Wilford Woodruff Academy | 760- North Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| Willow Creek Preschool | 4725 East Lake Drive, Winter Springs, FL 32708 | Preschool |
| Winegard Elementary School Preschool | 7055 Winegard Road, Orlando, FL 32809 | Preschool |
| Winners Children Academy | 6816 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Winners Preschool and Child Care Center III | 609 North Powers Drive Suite 308, Orlando, FL 32818 | Preschool |
| Winter Park Day Nursery | 741 South Pennsylvania Avenue, Winter Park, FL 32789 | Preschool |
| Winter Park Presbyterian Preschool | 400 South Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| YMCA Family Center at Disney - Buena Vista | 2800 East Vista Boulevard, Orlando, FL 32830 | Preschool |
| Young Achievers | 11 East Lancaster Road, Orlando, FL 32809 | Preschool |