| St. John's Day Care Ministry | 1505 St. John's Church Road, Lanesville, IN 47136 | Center |
| St. Joseph Child Development Center | 2823 Frankfort Avenue, Louisville, KY 40206 | Preschool |
| St. Lawrence Child Enrichment Center | 1925 Lewiston Drive, Louisville, KY 40216 | Preschool |
| St. Leonard Preschool | 440 Zorn Avenue, Louisville, KY 40206 | Preschool |
| St. Matthews Baptist Preschool | 3515 Grandview Avenue, Louisville, KY 40207 | Preschool |
| St. Matthews Child Development Center | 229 Breckenridge Lane, Louisville, KY 40207 | Preschool |
| St. Paul School | 6901 Dixie Highway, Louisville, KY 40258 | Preschool |
| St. Raphael Child Enrichment Center - CLOSED | 2140 Tyler Lane, Louisville, KY 40205 | Preschool |
| St. Raphael YMCA | 2131 Lancashire Avenue, Louisville, KY 40205 | Center |
| St. Rita Enrichment Center For Children | 8709 Preston Highway, Louisville, KY 40219 | Preschool |
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| St. Rita School | 8709 Preston Highway, Louisville, KY 40219 | Preschool |
| St. Stephen Martyr Out of School Program | 3015 Greenup Avenue, Louisville, KY 40217 | Preschool |
| Star Achievers Day Care | 3923 Bardstown Road, Louisville, KY 40218 | Preschool |
| Star Kids Child Care Learning Center | 4107 Cane Run Road, Louisville, KY 40216 | Preschool |
| Strathmoor Education Center | 2201 Hawthorne Avenue, Louisville, KY 40205 | Preschool |
| Sts. Simon & Jude Child Care | 4335 Hazelwood Avenue, Louisville, KY 40215 | Preschool |
| Stuart Middle Childcare Enrichment Program | 4601 Valley Station Road, Louisville, KY 40272 | Center |
| Success Learning Academy | 1001 Brookline Avenue, Louisville, KY 40215 | Preschool |
| Sunny Angels Day Care | 903 South 32nd Street, Louisville, KY 40211 | Preschool |
| Sunny Angels Day Care II | 856 South 32nd Street, Louisville, KY 40211 | Preschool |
| Taylor Tots Learning Center | 4301 Taylor Boulevard, Louisville, KY 40215 | Preschool |
| Temple Academy Daycare & Learning Center | 723 South 45th Street, Louisville, KY 40211 | Preschool |
| The Center at Riverport | 7401 Riverport Drive, Louisville, KY 40258 | Preschool |
| The Child Palace | 2607 Rodman Street, Louisville, KY 40208 | Preschool |
| The Children Center at Yum! Brands | 1900 Colonel Sanders Lane, Louisville, KY 40213 | Preschool |
| The Children's Learning Center | 1329 Applegate Lane, Clarksville, IN 47129 | Center |
| The Enrichment Center | 702 East Gray Street, Louisville, KY 40202 | Preschool |
| The HenEnManley Professional and International Childcare and Preschool Center | 228 Gheens Avenue, Louisville, KY 40214 | Preschool |
| The Jordan's Academy of Success | 4441 Manslick Road, Louisville, KY 40216 | Preschool |
| The Jordan's Academy of Success 2 | 4003 South Fifth Street, Louisville, KY 40214 | Preschool |
| The Learning Box | 8402 National Turnpike, Louisville, KY 40214 | Preschool |
| The Learning Tree Child Development Center | 1912 W. Broadway Street, Louisville, KY 40203 | Preschool |
| The Lively Children Childcare Center | 1140 Cherokee Road, Louisville, KY 40204 | Preschool |
| The Love's Learning Academy Center | 2708 Rockford Lane, Louisville, KY 40216 | Preschool |
| The Miracle Zone | 3510 St. Joseph Road, New Albany, IN 47150 | Center |
| The Miracle Zone II | 750 Highlander Point Drive, Floyds Knobs, IN 47119 | Center |
| The Potter's House Childcare Enrichment Center | 314 South Wenzel Street, Louisville, KY 40204 | Preschool |
| The Wright Place Day Care Center | 600 South 18th Street, Louisville, KY 40203 | Preschool |
| Thomas Jefferson Middle Childcare Enrichment Program | 1501 Rangeland Road, Louisville, KY 40219 | Center |
| Timothy's Angels Child Care Center | 1351 Catalpa Street, Louisville, KY 40211 | Preschool |
| Touch The Heart Child Development Center | 2718 Crums Lane, Louisville, KY 40216 | Preschool |
| Touching Small Hearts Daycare | 5406 Del Maria Way, Louisville, KY 40291 | Center |
| Trina's Treehouse II Childcare Center | 4443 Cane Run Road, Louisville, KY 40216 | Preschool |
| Trinity House Christian Child Care Campus | 4106 Greenwood Avenue, Louisville, KY 40211 | Preschool |
| Trinity House Christian Childcare Center | 844 Dearborn Avenue, Louisville, KY 40211 | Preschool |
| Trinity Learning Ministry | 2796 Charlestown Road, New Albany, IN 47150 | Center |
| Tyler's Playhouse | 3007 Dixie Highway A & B, Louisville, KY 40216 | Preschool |
| Tyler's Playhouse | 2922 Dixie Hwy, Louisville, KY 40216 | Preschool |
| Unseld Child Care & Development Center - Shively | 3950 Dixie Hwy, Louisville, KY 40216 | Preschool |
| Valley Child Care | 10105 Dixie Highway, Louisville, KY 40272 | Preschool |
| Valley View Child Development Center | 8911 Third Street Road, Louisville, KY 40272 | Preschool |
| Victory Christian Child Development Center | 2210 Woodland Avenue, Louisville, KY 40210 | Preschool |
| Village Child Enrichment Center | 1326 Berry Boulvard, Louisville, KY 40215 | Preschool |
| Visually Impaired Preschool Services | 1906 Goldsmith Lane, Louisville, KY 40218 | Preschool |
| Vivid Imaginations Child Care Center | 5925 Six Mile Lane, Louisville, KY 40218 | Preschool |
| Vivid Imaginations Child Care Center II | 3809 Bardstown Road, Louisville, KY 40218 | Preschool |
| WesKids Child Development Center | 5114 Preston Highway, Louisville, KY 40213 | Preschool |
| Weskids Child Development Center II | 2020 Garrs Lane, Louisville, KY 40216 | Preschool |
| When I Grow Up Child Care Center | 1400 Berry Blvd., Louisville, KY 40215 | Preschool |
| YMCA Christian Academy Of Louisville - English Station Program | 700 S. New English Station Rd., Louisville, KY 40245 | Center |
| YMCA Christian Academy of Louisville - Rock Creek SACC Program | 3110 Rock Creek Road, Louisville, KY 40207 | Center |
| YMCA St. Leonard Afterschool Program | 440 Zorn Avenue, Louisville, KY 40206 | Preschool |