| Jacksonville Urban League Early Headstart | 1626 Grunthal Street, Jacksonville, FL 32209 | Center |
| Jacksonville Urban League Head Start | 3811 St Augustine Road, Jacksonville, FL 32207 | Preschool |
| Jacksonville Urban League Normandy Heads | 6803 Arques Road, Jacksonville, FL 32205 | Preschool |
| Jewish Community Alliance | 8505 San Jose Boulevard, Jacksonville, FL 32217 | Center |
| John Love Elementary Preschool | 1531 Winthrop Street, Jacksonville, FL 32206 | Preschool |
| Joshua Christian Academy | 924 St. Clair St., Jacksonville, FL 32254 | Preschool |
| Juliette's Day Care | 6939 Como Road, Jacksonville, FL 32210 | Preschool |
| Just For Kids Learning Center | 11269 North Main Street, Jacksonville, FL 32218 | Preschool |
| Justina Road Elementary Preschool | 3101 Justina Road, Jacksonville, FL 32277 | Preschool |
| Juzt Kidz Learning Center | 8128 Lem Turner Road, Jacksonville, FL 32208 | Preschool |
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| Kats Little Kittens Learning Center | 3692 Beach Blvd., Jacksonville, FL 32207 | Preschool |
| Kias Little Angels | 5870-2&3 San Juan Boulevard, Jacksonville, FL 32210 | Preschool |
| Kid Kampus U S A | 1868 Parental Home Road, Jacksonville, FL 32216 | Preschool |
| Kid's Academy USA | 1842 Blanding Blvd Ste 3, Jacksonville, FL 32210 | Preschool |
| Kid'z on Top II Child Care & Learning Center | 5571 Playa Way, Jacksonville, FL 32211 | Preschool |
| Kiddie Care Day Care | 2020 Prospect Street, Jacksonville, FL 32208 | Center |
| Kiddieville Learning Center | 6820 Daughtry Blvd. South, Jacksonville, FL 32210 | Center |
| Kids Expanding the Kingdom Christian Preschool | 3749 Crownpoint Road, Jacksonville, FL 32257 | Preschool |
| Kids Konnection | 2221 Noble Street, Jacksonville, AL 36265 | Center |
| Kids Park | 4274 Hershel St., Jacksonville, FL 32210 | Preschool |
| Kids Point of View Learning Center | 2851 Edgewood Avenue North #12, Jacksonville, FL 32254 | Preschool |
| Kids United Childcare & Learning Center | 7037-1 Phillips Highway Suite 1, Jacksonville, FL 32216 | Preschool |
| Kids World Academy Westside Jacksonville | 4932 Blanding Blvd, Jacksonville FL, Jacksonville, FL 32210 | Center |
| Kids World of Florida | 6150 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Kids World of Florida | 2597 Oak Street, Jacksonville, FL 32204 | Preschool |
| Kids World of Florida | 4040 Confederate Point Road, Jacksonville, FL 32210 | Preschool |
| Kids in Dynamic Settings | 3271 Tiger Hole Road, Jacksonville, FL 32216 | Center |
| Kidsmart Christian Learning Center | 1901 Leonid Road, Jacksonville, FL 32218 | Preschool |
| Kidsville 2000 | 2553 Soutel Drive, Jacksonville, FL 32208 | Preschool |
| Kidz Klub Developmental Learning Center | 4804 Blanding Boulevard, Jacksonville, FL 32210 | Preschool |
| Kidz Korner Learning Center | 25 East 65th St, Jacksonville, FL 32208 | Preschool |
| Kidz Quest Academy | 1820 University Boulevard North, Jacksonville, FL 32211 | Preschool |
| Kidz n Kidz Academy | 6229 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Kimbari Learning Academy | 3651 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Kinder Kollege Learning Center | 1403 Myrtle Avenue North, Jacksonville, FL 32209 | Center |
| King's Kids Christian Learning Center | 135 S. Chaffee Rd, Jacksonville, FL 32220 | Preschool |
| King's Kids Learning Center | 9077 Lem Turner Road, Jacksonville, FL 32208 | Preschool |
| Kingdom Kare Learning Center | 5827 Seaboard Avenue, Jacksonville, FL 32244 | Center |
| Kingdom Kids Daycare | 6415c North Pearl Street, Jacksonville, FL 32208 | Preschool |
| Kingdom Kids Learning Center | 8668 Lem Turner Road, Jacksonville, FL 32208 | Preschool |
| Kingdom Kids at CrossRoad | 10005 Gate Parkway North, Jacksonville, FL 32246 | Preschool |
| Kings Kids Christian Learning Center | 135 Chaffee Road South, Jacksonville, FL 32220 | Center |
| Kings and Queens Child Care and Learning Center | 3325 Plateau Street, Jacksonville, FL 32206 | Center |
| Kyra's Child Development Center | 1029 Edgewood Ave N, Jacksonville, FL 32254 | Preschool |
| La Core Christian Academy | 122 Arlington Road, Jacksonville, FL 32211 | Preschool |
| La Escuelita Pre School | 5415 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Lakewood Presbyterian Church Preschool | 2001 University Boulevard West, Jacksonville, FL 32217 | Preschool |
| Lakewood United Methodist Preschool | 6133 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
| Lane Avenue Child Development Center | 1650 Lane Avenue, Jacksonville, FL 32210 | Preschool |
| Lang's Little Lambs Preschool | 5310 Lenox Ave Unit #26, Jacksonville, FL 32205 | Preschool |
| Learning Time Academy | 5301 Ricker Road, Jacksonville, FL 32210 | Preschool |
| Leslie's Angels | 2114 Tilden Street, Jacksonville, FL 32206 | Center |
| Lil Frenche's Christian Learning Center | 5013 Broadway Avenue, Jacksonville, FL 32254 | Preschool |
| Lil' Nuggets Daycare Center | 7054 Wilson Boulevard, Jacksonville, FL 32210 | Preschool |
| Lil' Sprouts Learning Center | 8840-7 Atlantic Blvd., Jacksonville, FL 32211 | Preschool |
| Little Blessings Academy | 1822 West 30th Street, Jacksonville, FL 32209 | Preschool |
| Little Bundles of Joy Learning Center and Daycare | 101 West 48th Street Suite 11, Jacksonville, FL 32208 | Preschool |
| Little Diamonds Big Heart Day Care | 1441 East 31st Street, Jacksonville, FL 32206 | Center |
| Little Disciples in Training Christian Childcare II | 3428 Winton Drive, Jacksonville, FL 32208 | Preschool |
| Little Dove Day Care & Learning Center | 7525 New Kings Road, Jacksonville, FL 32219 | Preschool |
| Little Friends | 4001 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Little Miracles Early Learning Academy | 101 - 8 West 48th Street, Jacksonville, FL 32208 | Preschool |
| Little People Child Care Center II | 5868 Lenox Avenue, Jacksonville, FL 32205 | Preschool |
| Little Royalty Learning Center | 4833 Plymouth Street, Jacksonville, FL 32205 | Center |
| Little Saints Child Care Center | 2179 Emerson Street, Jacksonville, FL 32207 | Preschool |
| Little Sarephims Daycare | 220 Mill Creek Road, Jacksonville, FL 32211 | Center |
| Little Temples Child Care Center II | 9100 Merrill Road Unit 15, Jacksonville, FL 32225 | Preschool |
| Little Tots Day Care | 6019 Lynnwood Avenue, Jacksonville, FL 32210 | Preschool |
| Little Wise Kids Early Learning Center | 6318 Arlington Road, Jacksonville, FL 32211 | Center |
| Little Wise Kids Early Learning Center II | 5703 Arlington Road, Jacksonville, FL 32211 | Center |
| Lollipop Kids Early Learning Center | 1233 Lane Avenue South #39, Jacksonville, FL 32205 | Preschool |
| Lone Star Learning Center | 8142 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Long Branch Elementary Preschool | 3723 Franklin Street, Jacksonville, FL 32206 | Preschool |
| Love-N-Care Day Care Center | 2948 Eden Street, Jacksonville, FL 32254 | Preschool |
| Lula's Kids | 1239 West Edgewood Avenue, Jacksonville, FL 32208 | Preschool |
| Magellan Christian Academies | 10550 - 704 Deerwood Park Boul ..., Jacksonville, FL 32256 | Preschool |
| Mandarin Prep School | 3968 Sunbeam Road, Jacksonville, FL 32257 | Preschool |
| McCrary's Learning Center | 6620 Arlington Expressway, Jacksonville, FL 32211 | Preschool |
| Merry Pop-Ins Child Care Center | 3935 St. Johns Avenue, Jacksonville, FL 32205 | Preschool |
| Methodist Children's VIllage | 7915 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Mine Yours & Ours Child Care Learning Center | 7645 Merrill Road #107, Jacksonville, FL 32277 | Preschool |
| Murray Hill Baptist Child Care | 4300 Post Street, Jacksonville, FL 32205 | Preschool |
| My First Step Learning Center | 10658 Biscayne Boulevard, Jacksonville, FL 32218 | Preschool |
| My First Step Learning Center II | 1638 Leonid Road, Jacksonville, FL 32218 | Preschool |
| Nakesa's Learning Center | 727 N Mcduff Avenue, Jacksonville, FL 32254 | Preschool |
| Nanny's Little Angels Learning Center | 8531 Normandy Blvd., Jacksonville, FL 32221 | Center |
| Nanny's Northside Nursery | 11033 Biscayne Blvd., Jacksonville, FL 32218 | Preschool |
| Naval Air Station Jacksonville Child Development Center | 705 Child Street, Jacksonville, FL 32212 | Preschool |
| New Beginning Day Care Center | 2804 West Edgewood Avenue, Jacksonville, FL 32209 | Preschool |
| New Dimension Christian Early Education Center | 5505 Normandy Bvld, Jacksonville, FL 32205 | Preschool |
| New Dimensions Learning Center | 3612 Belfort Road, Jacksonville, FL 32216 | Preschool |
| New Life In Christ Child Development Center | 6225 Norwood Avenue, Jacksonville, FL 32208 | Center |
| Normandy Village Elementary Preschool | 8257 Herlong Road, Jacksonville, FL 32210 | Preschool |
| Normandy Village Learning Academy | 8119 Herlong Road, Jacksonville, FL 32210 | Preschool |
| North Shore Elementary Preschool | 5701 Silver Plaza, Jacksonville, FL 32208 | Preschool |
| Northern Lights Sunshine Academy | 6240 Fort Caroline Road #1-4, Jacksonville, FL 32277 | Preschool |
| Northside Center For Creative Learning | 3534 Winton Drive, Jacksonville, FL 32208 | Preschool |
| Northside Community Involvement | 4736 Avenue B, Jacksonville, FL 32209 | Preschool |
| Oak Crest Child Care | 5900 Ricker Rd, Jacksonville, FL 32244 | Preschool |
| Oak Hill Early Learning Center | 4151 Old Middleburg Road N., Jacksonville, FL 32210 | Preschool |