| Tomorrow's Academy | 3121 Monte Carlo Trail, Orlando, FL 32805 | Center |
| Toni's Touch Pre-K | 1301 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Tots in Toyland Learning Center | 11235 West Michigan Street, Orlando, FL 32805 | Preschool |
| Trinity Child Development Center | 420 & 424 Ruth Ln, Orlando, FL 32801 | Preschool |
| Trinity Christian Academy | 2113 East South Street, Orlando, FL 32803 | Center |
| Trinity Christian Early Childhood | 1022 S Orange Blossom Trail, Apopka, FL 32703 | Preschool |
| True Deliverance Center Day Care | 29 South Ivey Lane, Orlando, FL 32811 | Center |
| Tutor Time Childcare Learning Center | 1025 South Clarke Road, Ocoee, FL 34761 | Preschool |
| Unique Child Care Center | 1373 Bunnell Rd, Apopka, FL 32703 | Preschool |
| United Cerebral Palsy - West Orange | 630 S Dillard Street, Winter Garden, FL 34787 | Preschool |
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| United Cerebral Palsy - Pine Hills Child Development | 5800 Golf Club Parkway, Orlando, FL 32818 | Preschool |
| United Cerebral Palsy of Central FL - Hollow | 3305 S. Orange Avenue, Orlando, FL 32806 | Preschool |
| Universal Child Care Development | 1003 South Kirkman Road, Orlando, FL 32811 | Preschool |
| Victory Christian Academy | 1601 A.d. Mims Rd, Ocoee, FL 34761 | Center |
| Washington Shores Elementary School Preschool | 944 West Lake Mann Drive, Orlando, FL 32805 | Preschool |
| Way of Grace Academy & Learning Center | 8550 Clarcona Ocoee Road, Orlando, FL 32818 | Center |
| We Love Kids | 636 N Wymore Road, Winter Park, FL 32789 | Preschool |
| Wee Kare Child Care Learning Academy | 5020 Silver Star Road, Orlando, FL 32808 | Preschool |
| Weekday School at First Presbyterian Church | 106 E. Church Street, Orlando, FL 32801 | Center |
| Welbourne Avenue Nursery & Kindergarten | 450 Welbourne Ave, Winter Park, FL 32789 | Preschool |
| Wesley Child Development Center | 42 E. Jackson Street, Orlando, FL 32801 | Preschool |
| West Oaks Elementary School Preschool | 905 Dorscher Rd, Orlando, FL 32818 | Preschool |
| West Orange Montessori School | 227 South Main Street, Winter Garden, FL 34787 | Center |
| West Orlando Baptist Child Discovery Center | 1006 E Crown Point Rd, Ocoee, FL 34761 | Center |
| Westside Community Church | 1937 Lakeville Road, Apopka, FL 32703 | Preschool |
| Wheatley Elementary School Preschool | 2 West 18th Street, Apopka, FL 32703 | Preschool |
| Wilford Woodruff Academy | 760- North Lakemont Avenue, Winter Park, FL 32792 | Preschool |
| Windermere Elementary Preschool | 11125 Park Avenue, Windermere, FL 34786 | Preschool |
| Windermere Union Church Preschool | 10710 Park Ridge Gotha Road, Windermere, FL 34786 | Preschool |
| Winners Children Academy | 6816 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Winners Preschool and Child Care Center III | 609 North Powers Drive Suite 308, Orlando, FL 32818 | Preschool |
| Winter Park Day Nursery | 741 South Pennsylvania Avenue, Winter Park, FL 32789 | Preschool |
| Winter Park Presbyterian Preschool | 400 South Lakemont Avenue, Winter Park, FL 32792 | Preschool |