| Aloma Methodist Early Childhood Learning Center | 3045 Aloma Avenue, Winter Park, Florida, Winter Park, FL 32792 | Preschool |
| Childtime of Winter Park | 1001 South Semoran Boulevard, Winter Park, FL 32792 | Preschool |
| Goldenrod Road KinderCare | 4035 N Goldenrod Rd, Winter Park, FL 32792 | Preschool |
| KinderCare Learning Center at Dr. Phillips | 7113 Wallace Rd, Orlando, FL 32819 | Center |
| KinderCare Orlando | 6537 Vista Park Blvd, Orlando, FL 32829 | Preschool |
| La Petite Academy of Orlando | 6573 Old Winter Garden Rd, Orlando, FL 32811 | Preschool |
| La Petite Academy of Orlando | 5066 S Conway Rd, Orlando, FL 32812 | Preschool |
| La Petite Academy of Orlando | 401 Mark Twain Blvd, Orlando, FL 32828 | Preschool |
| La Petite Academy of Orlando | 7303 Lake Underhill, Orlando, FL 32822 | Preschool |
| La Petite Academy of Orlando | 10936 South Trail Circle, Orlando, FL 32837 | Preschool |
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| La Petite Academy of Orlando | 14524 Gatorland Drive, Orlando, FL 32837 | Preschool |
| A Child's Place | 2301 E Michigan St, Orlando, FL 32806 | Preschool |
| A Place 4 Tots | 1427 N Pine Hills Road, Orlando, FL 32808 | Preschool |
| ABC Learning Center and Child Care | 2310 East Concord Street, Orlando, FL 32803 | Preschool |
| ABC Learning Tree | 2778 South Westmoreland Drive, Orlando, FL 32805 | Preschool |
| Academy 2000 | 925 South Ivey Lane, Orlando, FL 32811 | Center |
| Academy 2000 | 624 Bethune Drive, Orlando, FL 32805 | Preschool |
| Adorable Treasures Academy | 4750 Silver Star Road, Orlando, FL 32808 | Center |
| Adorable Treasures Pre-School Academy | 4801 Basswood Lane, Orlando, FL 32808 | Preschool |
| Adorable Treasures Preschool | 4750 Silver Star Road, Orlando, FL 32808 | Preschool |
| Advanced Beginnings Childcare Development | 406 E. Amelia Street, Orlando, FL 32803 | Preschool |
| Adventure Preschool and Daycare | 4652 South Orange Blossom Trail, Orlando, FL 32839 | Preschool |
| All God's Angels Preschool & Child Care | 1215 Mercy Drive, Orlando, FL 32808 | Preschool |
| All Saints School | 338 East Lyman Avenue, Winter Park, FL 32789 | Preschool |
| Aloma Elementary School Preschool | 2949 Scarlet Road, Winter Park, FL 32789 | Preschool |
| Aloma Kids Academy | 3416 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Aloma Kids of Orlando | 3416 Aloma Ave, Winter Park, FL 32792 | Preschool |
| American Pre-schools | 4000 South Goldenrod Road, Orlando, FL 32822 | Preschool |
| Angel Sprouts Academy | 7651 Valencia College Lane, Orlando, FL 32807 | Preschool |
| Angel's Child Care Center | 4819 Skeena Street, Orlando, FL 32819 | Preschool |
| Apple Academy | 1400 Jordan Ave, Orlando, FL 32809 | Preschool |
| Arcoiris De Amor Child Care | 929 W. Michigan Street, Orlando, FL 32805 | Center |
| Arising Little Angels Preschool | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Arising Little Angels Preschool Academy | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Atonement Lutheran Preschool | 8849 Passaic Parkway, Orlando, FL 32829 | Center |
| Audubon Elementary School Preschool | 1750 Common Way Rd, Orlando, FL 32814 | Preschool |
| Augustina Academy | 1307 Pine Hills Road, Orlando, FL 32808 | Preschool |
| Aunt Delores's Child Care | 1411 Melanie Drive, Orlando, FL 32825 | Preschool |
| Aunt Mary's Child Development Center Orlando | 840 Hoffner Avenue, Orlando, FL 32809 | Center |
| Azalea Park Baptist School | 5725 Dahlia Dr, Orlando, FL 32807 | Preschool |
| Azalea Park Elementary School Preschool | 1 Carol Avenue, Orlando, FL 32807 | Preschool |
| Azalea Park Learning Center | 940 South Semoran Boulevard, Orlando, FL 32807 | Preschool |
| BETA Center Developmental Childcare | 4680 Lake Underhill Rd, Orlando, FL 32807 | Preschool |
| Baldwin Oaks Academy | 1862 Winter Park Road, Orlando, FL 32803 | Center |
| Bays Childcare Center | 1239 North Pine Hills Road, Orlando, FL 32808 | Center |
| Beatrice's Guardian Angel | 900 West Lancaster Road Suite 1 ..., Orlando, FL 32809 | Preschool |
| Best Memories Academy Child Care Center | 5135 Curry Ford Road, Orlando, FL 32812 | Preschool |
| Bethany Educational Center | 6229 Winegard Rd, Orlando, FL 32809 | Preschool |
| Big Children's Academy | 3300 S. Conway Road, Orlando, FL 32812 | Preschool |
| Big Stars Learning Center | 430 West Lancaster Road, Orlando, FL 32809 | Preschool |
| Black Belt Center of Orlando | 4516 Hoffner Ave, Orlando, FL 32812 | Center |
| Bright Horizons at Baldwin Park | 1700 Fire House Lane, Orlando, FL 32814 | Preschool |
| Bright Stars Preschool | 7325 Conroy Windermere Road, Orlando, FL 32835 | Preschool |
| Brighter Beginnings Learning Center | 5607 Hansel Avenue, Orlando, FL 32809 | Preschool |
| Brush Arbor Christian | 2304 North Goldenrod Road, Orlando, FL 32807 | Center |
| CHS A Place For Children | 425 North Orange Avenue R270 S ..., Orlando, FL 32801 | Center |
| Callahan Headstart | 101 North Parramore Avenue, Orlando, FL 32801 | Preschool |
| Catalina Elementary School Preschool | 2448 W. 29th Street, Orlando, FL 32805 | Preschool |
| Cheney Elementary School Preschool | 2000 N. Forsyth Road, Orlando, FL 32807 | Preschool |
| Chicksaw Elementary School Preschool | 6900 Autumnvale Dr., Orlando, FL 32822 | Preschool |
| Childlife Preschool | 5495 Lake Margaret Drive, Orlando, FL 32812 | Preschool |
| Children 1st Preschool | 11648 McCulloch Rd, Orlando, FL 32817 | Center |
| Children Palace Learning Center | 1230 North Pine Hills Rd, Orlando, FL 32808 | Preschool |
| Children's Academy at Loch Haven | 200 E Evans St, Orlando, FL 32804 | Preschool |
| Children's Academy at Loch Haven - Mildly Ill | 227 East Evans Street, Orlando, FL 32804 | Preschool |
| Children's Kingdom | 426 W Lancaster Rd, Orlando, FL 32809 | Preschool |
| Children's Palace | 1800 W Washington St, Orlando, FL 32805 | Center |
| Christ The King Lutheran Preschool | 4962 South Apopka Vineland Road, Orlando, FL 32819 | Preschool |
| Coalition For The Homeless | 639 W Central Blvd, Orlando, FL 32801 | Preschool |
| College Park Kiddie Kampus | 2208 N Rio Grande Ave, Orlando, FL 32804 | Center |
| College Park U M Child Dev Center | 44 West Princeton Street, Orlando, FL 32804 | Preschool |
| Columbia Elementary School Preschool | 18501 Cypress Lake Glen, Orlando, FL 32801 | Preschool |
| Columbus Center | 4629 Middlebrook Road, Orlando, FL 32811 | Preschool |
| Community Church Preschool | 3839 S Ferncreek Ave, Orlando, FL 32806 | Center |
| Conway Learning Center | 3636 E Michigan St, Orlando, FL 32812 | Preschool |
| Conway United Methodist Preschool | 3401 S Conway Rd, Orlando, FL 32812 | Preschool |
| Creativitown Day Care | 2000 Falcon Trace Blvd Ste #100, Orlando, FL 32837 | Preschool |
| Da Vinci Academy | 1901 Park Center Drive, Orlando, FL 32835 | Preschool |
| DaVinci Preschool Academy | 1901 Park Center Drive, Orlando, FL 32835 | Preschool |
| Delaney Early Learning Center | 1919 Delaney Ave, Orlando, FL 32809 | Center |
| Discover Academy | 319 South Lakewood Drive, Orlando, FL 32803 | Preschool |
| Discovery Prep Academy | 5715 Turkey Lake Road, Orlando, FL 32819 | Preschool |
| Discovery Zone Preschool | 11333 Lake Underhill Rd, Orlando, FL 32825 | Preschool |
| Divine Individuals Developmental Center | 140 South West Avenue, Maitland, FL 32751 | Preschool |
| Divine Orders Preschool & Child Care | 913 South Ivey Lane, Orlando, FL 32811 | Preschool |
| Divine Orders Preschool and Childcare | 913 South Ivey Lane, Orlando, FL 32811 | Preschool |
| Downey Christian Pre-School | 10201 E. Colonial Drive, Orlando, FL 32817 | Preschool |
| Dr Phillips Elementary School Preschool | 6909 Dr Phillips Blvd, Orlando, FL 32819 | Preschool |
| Dreams Castle | 1530 South Primrose Drive, Orlando, FL 32806 | Preschool |
| Drop of Love | 207 West Lancaster Road, Orlando, FL 32809 | Preschool |
| Eagle's Nest Elementary School Preschool | 5353 Metro West Blvd, Orlando, FL 32811 | Preschool |
| Early Learning Center at First Orlando | 3000 S. John Young Pkwy, Orlando, FL 32805 | Center |
| Eastbrook Elementary Vpk - Seminole County | 5525 Tangerine Avenue, Winter Park, FL 32792 | Preschool |
| Eastland Christian School | 9000 Lakeunder Hill Road, Orlando, FL 32825 | Center |
| Eccleston Elementary School Preschool | 1500 Aaron Ave, Orlando, FL 32811 | Preschool |
| Einstein Academy - Campus 2 | 12046 Collegiate Way, Orlando, FL 32817 | Preschool |
| Einstein Academy at La Vina | 9145 Narcoossee, Orlando, FL 32827 | Preschool |
| Elite Preparatory Academy | 730 Sand Lake Road, Orlando, FL 32809 | Preschool |
| Engelwood Elementary School Preschool | 900 Engel Dr, Orlando, FL 32807 | Preschool |
| Erma's Day Care Center of Orlando | 1819 Williams Manor, Orlando, FL 32811 | Preschool |