| San Jose Catholic School | 3619 Toledo Road, Jacksonville, FL 32217 | Preschool |
| San Juan Del Rio Catholic School | 1714 State Road 13, Saint Johns, FL 32259 | Preschool |
| Seventh Generation Academy | 10679 Old St. Augustine Rd, Jacksonville, FL 32257 | Preschool |
| Sharing Tree Preschool | 6045 Greenland Road, Jacksonville, FL 32258 | Preschool |
| Sharing Tree Preschool at Fleming Island | 5900 Highway 17 South, Fleming Island, FL 32003 | Preschool |
| Shepherd of the Woods Lutheran School | 7860 Southside Blvd, Jacksonville, FL 32256 | Preschool |
| Shepherd of the Woods Lutheran School II | 6595 Columbia Park St., Jacksonville, FL 32258 | Preschool |
| St. Giles Children's Center | 116 Foxridge Rd., Orange Park, FL 32065 | Preschool |
| St. Margaret's Children's House | 6865 Pine Avenue, Fleming Island, FL 32003 | Preschool |
| Stepping Stones Preschool | 6233 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
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| Sugar N Spice | 3211 Moody Avenue, Orange Park, FL 32065 | Preschool |
| Sunshine Academy of Orange Park | 782 Foxridge Center Dr., Orange Park, FL 32065 | Preschool |
| T's Learning Center III | 10915 Baymeadows Road Unit 200, Jacksonville, FL 32256 | Preschool |
| The Academy at Julington Creek | 990 Flora Branch Blvd, Saint Johns, FL 32259 | Preschool |
| The Cambridge Prep School | 12246 San Jose Blvd., Jacksonville, FL 32223 | Preschool |
| The Children's Corner | 1720 Smith Street, Orange Park, FL 32073 | Preschool |
| The Goddard School | 100 Julington Plaza Drive, Saint Johns, FL 32259 | Preschool |
| The Goddard School of Fleming Island | 2280 Village Square Parkway, Fleming Island, FL 32003 | Preschool |
| The Learning Experience | 8411-100 Southside Blvd, Jacksonville, FL 32256 | Preschool |
| The Learning Tree Preschool Center | 6140 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
| The Sunshine House | 3993 San Jose Park Drive., Jacksonville, FL 32217 | Preschool |
| Torah Academy of Jacksonville | 10167 San Jose Blvd, Jacksonville, FL 32257 | Preschool |
| W E Cherry Elementary School Preschool | 420 Edison Drive, Orange Park, FL 32073 | Preschool |
| Wonderworks OPUMC | 2036 Reed Street, Orange Park, FL 32073 | Preschool |
| World of Wonders Academy | 101 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| YMCA Pryme Time at Argyle Elementary | 2625 Spencer Plantation Blvd, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at C.E. Bennett | 1 South Oakridge Avenue, Green Cove Springs, FL 32043 | Center |
| YMCA Pryme Time at Drs. Inlet Elementary | 2634 State Road 220, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Fleming Island Elementary | 4425 Lake Shore Drive, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Grove Park Elementary | 1643 Miller Street, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Jessie Ball Dupont | 7373 Old Kings Road South, Jacksonville, FL 32217 | Center |
| YMCA Pryme Time at Lakeside Elementary | 2752 Moody Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Montclair Elementary | 2398 Moody Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Orange Park Elementary | 1401 Plainfield Avenue, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Paterson Elementary | 5400 Pine Avenue, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Ridgeview Elementary | 421 Jefferson Ave., Orange Park, FL 32065 | Center |
| YMCA Pryme Time at SB Jennings Elementary | 215 Corona Drive, Orange Park, FL 32073 | Center |
| YMCA Pryme Time at Swimming Pen Creek | 1630 Woodpecker Lane, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Thunderbolt Elementary | 2020 Thunderbolt Road, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at W.E. Cherry Elementary | 420 Edson Drive, Orange Park, FL 32073 | Center |