| Tomorrow's Stars Learning Center | 4027 Moncrief Road, Jacksonville, FL 32209 | Center |
| Tonia's Precious Tots | 1286 Dunn Ave, Jacksonville, FL 32218 | Preschool |
| Top Notch Kids Learning Center | 2726 North Main Street, Jacksonville, FL 32206 | Preschool |
| Trinity Christian Academy | 800 Hammond Boulevard, Jacksonville, FL 32221 | Preschool |
| True Vine Children's Center | 3519 Post Street, Jacksonville, FL 32205 | Preschool |
| Tyler Kennedi Learning Center | 814 Ellis Road, Jacksonville, FL 32205 | Preschool |
| UPC - Rainbows & Ribbons | 5152 Vernon Road, Jacksonville, FL 32209 | Preschool |
| Victorious Learning Center | 2629 Edison Avenue, Jacksonville, FL 32204 | Preschool |
| Victory Baptist Learning Center | 10613 Lem Turner Road, Jacksonville, FL 32218 | Preschool |
| Victory Christian Academy | 10613 Lem Turner Road, Jacksonville, FL 32218 | Center |
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| Wayman Early Learning Academy | 1176 Labelle Street, Jacksonville, FL 32205 | Preschool |
| Wayman Early Learning Academy - Northside | 4811 Payne Steward Drive, Jacksonville, FL 32209 | Center |
| Wesley Child Development Center | 1955 Broward Road, Jacksonville, FL 32218 | Preschool |
| West Jacksonville Elementary Preschool | 2115 Commonwealth Avenue, Jacksonville, FL 32209 | Preschool |
| Wilma's Little People's School | 2800 University Boulevard North, Jacksonville, FL 32211 | Center |
| Wonderland Learning Center | 15 East 25th Street, Jacksonville, FL 32206 | Preschool |
| Wright's Learning Academy | 687 Edgewood Ave N, Jacksonville, FL 32254 | Preschool |
| YMCA Pryme Time at Panther Enrichment Center | 1709 Landsdowne Drive, Jacksonville, FL 32211 | Center |
| Young Hearts Child Development Center II | 4800 North Main Street, Jacksonville, FL 32206 | Preschool |
| Young Start Academy | 1049 Palmetto Street, Jacksonville, FL 32206 | Preschool |
| Zarephath Learning Center | 1028 East 10th Street, Jacksonville, FL 32206 | Center |
| growing minds development center | 1181 cassat ave, Jacksonville, FL 32205 | Center |