| Childtime of Jacksonville | 8500 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Childtime of Jacksonville | 9950 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Childtime of Orange Park | 1510 Business Center Drive, Fleming Island, FL 32003 | Preschool |
| Eagle Harbor Parkway KinderCare | 1735 Eagle Harbor Pkwy, Fleming Island, FL 32003 | Preschool |
| La Petite Academy of Jacksonville | 6601 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| La Petite Academy of Middleburg | 3150 County Road 220, Middleburg, FL 32068 | Preschool |
| La Petite Academy of Orange Park | 4321 US Highway 17, Fleming Island, FL 32003 | Preschool |
| Oakleaf KinderCare | 431 Drysdale, Orange Park, FL 32065 | Center |
| Old St Augustine Rd KinderCare | 4310 Barkoskie Rd, Jacksonville, FL 32258 | Preschool |
| Tutor Time of Jacksonville | 13734 St. Augustine Road, Jacksonville, FL 32258 | Preschool |
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| Tutor Time of Jacksonville | 8510 Watermill Boulevard South, Jacksonville, FL 32244 | Preschool |
| 1st Journey Learning Center | 1759 Parkwood St, Jacksonville, FL 32207 | Preschool |
| A Bright Beginning Child Care | 8386 Baymeadow Road Suite 100, Jacksonville, FL 32256 | Preschool |
| A Bright Beginning Childcare | 8625 Old Kings Road South Suite ..., Jacksonville, FL 32217 | Center |
| A Child's Place Montessori School | 3718 Salisbury Road, Jacksonville, FL 32216 | Preschool |
| A Child's Place Montessori School (Mandarin) | 12601 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| A Gift From God Child Development Center | 2104 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| A Little Preschool House | 4504 Sunderland Road, Jacksonville, FL 32210 | Preschool |
| A New Beginning Child Care | 2528 W. Beaver St., Jacksonville, FL 32254 | Preschool |
| A Precious Gift From God Child Development Center | 1785 Cassat Ave, Jacksonville, FL 32210 | Center |
| ABC Learning Center of Jacksonville | 8341 Herlong Road, Jacksonville, FL 32210 | Preschool |
| ABCD Center | 735 S. Mcduff Ave, Jacksonville, FL 32205 | Center |
| Abbate's Day Care | 349 Blanding Boulevard, Orange Park, FL 32073 | Preschool |
| Abundantly Loved Early Preschool Academy | 5154 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Academie de Montessori | 1216 Lasalle Street, Jacksonville, FL 32207 | Preschool |
| Advent Lutheran Preschool | 2156 Loch Rane Boulevard, Orange Park, FL 32073 | Preschool |
| All Saints Early Learning & Community Center | 4171 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Alpha Omega Learning Center | 2004 Hyde Park Road, Jacksonville, FL 32210 | Preschool |
| Amazing Kids Learning Center | 3509 Kingston St., Jacksonville, FL 32254 | Preschool |
| Andromeda Preschool | 131 Suzanne Avenue, Orange Park, FL 32073 | Preschool |
| Angels Elite Academy | 3560 Marbon Road, Jacksonville, FL 32223 | Preschool |
| Annie R. Morgan Elementary Preschool | 964 St. Clair Street, Jacksonville, FL 32254 | Preschool |
| Annunciation Catholic School | 1610 Blanding Blvd., Middleburg, FL 32068 | Preschool |
| Apple Tree Academy Childcare | 1246 Park Avenue, Orange Park, FL 32073 | Center |
| Argyle Christian Preschool and Kindergarten | 6823 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Arora's Clubhouse | 330 Arora Boulevard, Orange Park, FL 32073 | Preschool |
| Assumption Catholic School | 2431 Atlantic Boulevard, Jacksonville, FL 32207 | Preschool |
| Atcom Child Care Learning Center | 5512 Westconnett Blvd, Jacksonville, FL 32244 | Center |
| Avondale United Methodist Child's Day Out | 1651 Talbot Avenue, Jacksonville, FL 32205 | Preschool |
| Backpack University | 400 College Drive, Middleburg, FL 32068 | Center |
| Baymeadows Baptist Day School | 4826 Baymeadows Road, Jacksonville, FL 32217 | Preschool |
| Baymeadows KinderCare - CLOSED | 8401 Baymeadows Way, Jacksonville, FL 32256 | Preschool |
| Belmont Head Start | 15 Belmont Blvd, Orange Park, FL 32073 | Preschool |
| Biltmore Elementary Preschool | 2101 West Palm Avenue, Jacksonville, FL 32254 | Preschool |
| Blueprints for life Childcare Academy | 11367 Old St. Augustine Road, Jacksonville, FL 32257 | Preschool |
| Bosman Family Child Care Center | 5150 Timuquanna Road Unit 1, Jacksonville, FL 32210 | Preschool |
| Bright Future Learning Center | 8062 Old Kings Road South, Jacksonville, FL 32217 | Preschool |
| Bright Scholars Learning Center | 3003 Claire Lane, Jacksonville, FL 32223 | Preschool |
| Bright Start School and Daycare | 217 Wells Road, Orange Park, FL 32073 | Center |
| Builders of the Faith Christian Daycare | 7540 103rd Street #216, Jacksonville, FL 32210 | Preschool |
| Burrell's Child Care Center | 4162 Spring Park Circle, Jacksonville, FL 32207 | Preschool |
| CHILDRENS FANTASY WORLD | 438 smith street, Jacksonville, FL 32204 | Center |
| Cedar Hills Baptist Christian School | 4200 Jammes Road, Jacksonville, FL 32210 | Center |
| Challenge Enterprises Lighthouse Learning | 550 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Chappell Child Development Center | 8400 Baycenter Road, Jacksonville, FL 32256 | Preschool |
| Chappell Premier Schools - Riverside | 484 Riverside Avenue, Jacksonville, FL 32202 | Preschool |
| Chappell at Florida State College at Jacksonville | 3939 Roosevelt Boulevard, Jacksonville, FL 32205 | Preschool |
| Chappell at Riverplace | 1301 Riverplace Boulevard, Jacksonville, FL 32207 | Preschool |
| Children's Fantasy World Pre-School | 438 Smith Street, Jacksonville, FL 32204 | Preschool |
| Children's Gifting Circle Child Development Center | 1561 Cassat Ave., Jacksonville, FL 32210 | Center |
| Childrenville Learning Center | 5576 Timuquana Road #101, Jacksonville, FL 32210 | Preschool |
| Childtime Learning Center | 2890 Moody Avenue, Orange Park, FL 32073 | Preschool |
| Chosen Ones Learning Center | 4953 Firestone Road, Jacksonville, FL 32210 | Preschool |
| Chosen Vessels Christian Academy Preschool | 4545-3 Shirley Avenue, Jacksonville, FL 32210 | Preschool |
| Cindy C's Day Nursery & Child Care | 9044 Crystal Springs Road, Jacksonville, FL 32221 | Preschool |
| Community Academy of Jacksonville | 2655 Community Road, Jacksonville, FL 32207 | Preschool |
| Community Connections of Jacksonville | 325 East Duval Street, Jacksonville, FL 32202 | Preschool |
| Cornerstone Christian School VPK | 4000 Spring Park Rd, Jacksonville, FL 32207 | Preschool |
| Country Bumpkins Child Care | 10805 Old Gainesville Road, Jacksonville, FL 32221 | Preschool |
| D C Academy | 2642 Firestone Road, Jacksonville, FL 32210 | Center |
| D L C Nurse & Learn | 4101 College Street, Jacksonville, FL 32205 | Preschool |
| Dawn's Daycare and Learning Center | 1775 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| Diamond In A Rough Learning Center | 142 Lane Avenue South, Jacksonville, FL 32254 | Preschool |
| Doctors Inlet Elementary Preschool | 2634 Cr 220, Middleburg, FL 32068 | Preschool |
| Every Child Can Learn Child Development Center | 8301 West Beaver Street, Jacksonville, FL 32220 | Preschool |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| First Baptist Junior Academy | 125 West Ashley St, Jacksonville, FL 32202 | Preschool |
| First Christian Preschool | 11924 San Jose Blvd, Jacksonville, FL 32223 | Preschool |
| First Class Learning Center | 939 Blanding Blvd., Orange Park, FL 32065 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Fleming Island Elementary School Preschool | 4425 Lakeshore Drive, Fleming Island, FL 32003 | Preschool |
| Flower Garden Early Education | 3602 Park Street, Jacksonville, FL 32205 | Center |
| Footprints In The Sand Child Development Center | 1303 North Edgewood Avenue, Jacksonville, FL 32254 | Preschool |
| Fox Meadows Chrisitian Daycare | 3227 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Fruit Cove Baptist Weekday Preschool | 501 State Road 13, Saint Johns, FL 32259 | Center |
| G E B Momma's House Academy Private School | 5431 Lenox Avenue, Jacksonville, FL 32205 | Center |
| Gateway Community Services | 555 Stockton Street, Jacksonville, FL 32204 | Center |
| Gingerbread House Learning Center | 6005 Chester Ave., Jacksonville, FL 32217 | Preschool |
| Gingerbread Learning Center | 30 Knight Boxx Road, Orange Park, FL 32065 | Preschool |
| God's Little People Christian Academy | 5844 Normandy Boulevard, Jacksonville, FL 32205 | Preschool |
| Good Samaritan Learning Tree | 3813 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Good Shepherd Lutheran Church Precious Lambs Preschool | 6551 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Grace Episcopal Dayschool | 156 Kingsley Avenue, Orange Park, FL 32073 | Center |
| Graham Bears Childcare | 5151 Colonial Ave., Jacksonville, FL 32210 | Preschool |
| Grove Park Elementary School Preschool | 1643 Miller Street, Orange Park, FL 32073 | Preschool |
| Growing Minds Development Center | 1181 Cassat Avenue, Jacksonville, FL 32205 | Center |
| Happy Hands Christian Child Care | 2017 Lane Avenue South, Jacksonville, FL 32210 | Preschool |
| Head Start at Little Angels In Paradise | 3252 N Canal Street, Jacksonville, FL 32209 | Preschool |
| Head Start at Old Stanton | 521 West Ashley Street, Jacksonville, FL 32202 | Preschool |
| Head Start at Pearson | 1314 West Union Street, Jacksonville, FL 32209 | Preschool |