| Bell Shoals KinderCare | 4304 Bell Shoals Rd, Valrico, FL 33596 | Preschool |
| La Petite Academy of Brandon | 808 W. Bloomingdale Avenue, Brandon, FL 33511 | Preschool |
| La Petite Academy of Brandon | 606 E. Windhorst Road, Brandon, FL 33510 | Preschool |
| La Petite Academy of Riverview | 10025 Cristina Drive, Riverview, FL 33569 | Preschool |
| La Petite Academy of Valrico | 1011 Greenhollow Lane, Valrico, FL 33596 | Center |
| Seffner KinderCare | 2524 S Parsons Ave, Seffner, FL 33584 | Preschool |
| A Child's Haven Private Preschool | 1520 Brentwood Hills Boulevard, Valrico, FL 33594 | Preschool |
| A Children's Kastle Early Learning Center | 950 S. Mulrennan Road, Valrico, FL 33594 | Preschool |
| A Plus Child Care Center | 3042 S. 78th Street, Tampa, FL 33619 | Center |
| All Nations Preschool | 10022 Cristina Drive, Riverview, FL 33569 | Preschool |
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| Almand Center | 1311 Brooker Road, Brandon, FL 33511 | Preschool |
| Apostles Lutheran Church Preschool | 200 N. Kingsway Road, Brandon, FL 33510 | Preschool |
| B & G Day Care and Kindergarten | 14419 Dr Mlk Boulevard, Dover, FL 33527 | Preschool |
| Bell Shoals Baptist Academy | 2102 Bell Shoals Road, Brandon, FL 33511 | Center |
| Bloomingdale Academy | 11418 Bloomingdale Avenue, Riverview, FL 33578 | Center |
| Boys & Girls Club Dover | 2820 South Gallagher Road, Dover, FL 33527 | Center |
| Brandon Academy | 801 Limona Road, Brandon, FL 33510 | Preschool |
| Brandon Day School | 202 Pauls Drive, Brandon, FL 33511 | Center |
| Brandon Sports and Aquatic Center | 405 Beverly Blvd, Brandon, FL 33511 | Preschool |
| Brighter Kids | 2207 Durant Road, Valrico, FL 33596 | Center |
| Browns Town Daycare | 7739 GIBSONTON DR, Gibsonton, FL 33534 | Center |
| Castles of Imagination | 820 Bryan Road, Brandon, FL 33511 | Preschool |
| Central Baptist Christian School | 402 E. Windhorst Road, Brandon, FL 33510 | Center |
| Child-Kare Solutions | 11002 E. Broadway Avenue, Seffner, FL 33584 | Preschool |
| Childcare of Brandon | 730 Brandon Town Center Dr, Brandon, FL 33511 | Center |
| Children's Academy | 506 Limona Road, Brandon, FL 33510 | Preschool |
| Children's Life Academy | 3120 S. Miller Road, Valrico, FL 33596 | Center |
| Children's Nest Day School | 3221 S. Bryan Road, Brandon, FL 33511 | Preschool |
| Circle M Ranch Day Care | 1202 Bloomingdale Avenue, Valrico, FL 33596 | Preschool |
| Colour Their World | 115-a Margaret Street, Brandon, FL 33511 | Preschool |
| Creative World School Fish Hawk | 5525 Osprey Ridge Drive, Lithia, FL 33547 | Preschool |
| Creative World School at Rivercrest | 11361 Symmes Road, Riverview, FL 33569 | Preschool |
| Crossroads Baptist Church | 10405 S. Highway 39, Lithia, FL 33547 | Center |
| Discovery Point #54 | 3472 Kings Commercial Ct, Valrico, FL 33594 | Preschool |
| Discovery Point #60 | 11719 Symmes Road, Riverview, FL 33569 | Preschool |
| Discovery Point #70 | 13011 Summerfield Crossing Boulevard, Riverview, FL 33579 | Preschool |
| East Bay Christian School | 10102 Old Big Bend Road, Riverview, FL 33578 | Center |
| Faith Baptist Preschool | 1118 North Parsons Avenue, Brandon, FL 33510 | Center |
| First Baptist Brandon Christian Academy | 216 N. Parsons Ave., Brandon, FL 33510 | Center |
| First Discoveries Academy | 1311 Brooker Road, Brandon, FL 33511 | Center |
| First Presbyterian Preschool of Brandon | 121 Carver Street, Brandon, FL 33510 | Preschool |
| First UMChurch Preschool and Kindergarten | 120 N. Knights Avenue, Brandon, FL 33510 | Preschool |
| Fish Hawk Early Learning Center | 5632 Osprey Park Place, Lithia, FL 33547 | Preschool |
| Fish Hawk Early Learning Center II | 6001 Parkset Drive, Lithia, FL 33547 | Preschool |
| Foundation Christian Academy | 3955 Lithia Pinecrest Road, Valrico, FL 33596 | Preschool |
| Good Samaritan Mission | 14920 Balm Wimauma Road, Wimauma, FL 33598 | Preschool |
| HCPS - Doby Elementary Headstart Vpk | 6720 Covington Garden Drive, Apollo Beach, FL 33572 | Preschool |
| HCPS - Dover Elementary Headstart Vpk | 3035 Nelson Ave., Dover, FL 33527 | Preschool |
| HCPS - Gibsonton Elem Headstart Vpk | 7723 Gibsonton Drive, Gibsonton, FL 33534 | Preschool |
| HCPS - Trapnell Elementary Headstart Vpk | 1605 West Trapnell Rd, Plant City, FL 33567 | Preschool |
| Happy Day Preschool | 722 S. Lithia Pinecrest Road, Brandon, FL 33511 | Preschool |
| Harvest Time Christian School | 1511 S. Us 301, Tampa, FL 33619 | Center |
| Harvest Time Christian School | 1511 U S 301 South, Tampa, FL 33619 | Center |
| Heaven Sent Day Care Center | 3003 S. Kings Avenue, Brandon, FL 33511 | Center |
| Home Away From Home Learning Center | 8516 Ridein Road, Tampa, FL 33619 | Center |
| Horizon Early Learning Preschool | 1720 S. Saint Cloud Avenue, Valrico, FL 33594 | Preschool |
| Immanuel Lutheran Pre-school | 2913 John Moore Road, Brandon, FL 33511 | Preschool |
| It's Kidz Time Of Riverview | 11329 Big Bend Road, Riverview, FL 33569 | Center |
| Kid's Community College - Lake St. Charles | 10544 Lake St. Charles Blvd, Riverview, FL 33578 | Preschool |
| Kid's Community College FishHawk Preschool | 16641 Fishhawk Boulevard, Lithia, FL 33547 | Preschool |
| Kids R Kids #8 FL | 4321 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| Kids R Kids Southshore | 13151 Kings Lake Drive, Gibsonton, FL 33534 | Preschool |
| Kids R Tops | 215 N. Kingsway Road, Brandon, FL 33510 | Center |
| Kinder Kountry | 12660 Sydney Road, Dover, FL 33527 | Center |
| Kings Avenue Christian School | 2602 S. Kings Avenue, Brandon, FL 33511 | Center |
| Krestview Academy | 11425 Balm Riverview Road, Riverview, FL 33569 | Preschool |
| Lamb of God's Open Arms Early Childhood Center | 10540 Browning Road, Lithia, FL 33547 | Preschool |
| Lambkins | 1017 Kingsway Rd Rd Brandon, Brandon, FL 33510 | Center |
| Learn & Grow Pre-school | 2501 Durant Road, Valrico, FL 33596 | Preschool |
| Limona Village Chapel Children's Center | 408 Limona Road, Brandon, FL 33510 | Preschool |
| Little Footsteps Academy of Bloomingdale | 3721 Canoga Park Drive, Brandon, FL 33511 | Preschool |
| Little Giants Learning Academy | 10891 Hackney Drive, Riverview, FL 33578 | Center |
| Little Stars Learning Academy | 706 N. Parsons Avenue, Brandon, FL 33510 | Center |
| Mango Head Start EHS | 11210 E. Broadway Avenue, Seffner, FL 33584 | Preschool |
| Mary Go Round Child Care Center | 10810 Hanaway Drive, Riverview, FL 33578 | Preschool |
| Mary Go Round-South Pointe | 10615 Southern Pointe Boulevard, Riverview, FL 33578 | Preschool |
| Mis' Ginny's | 1502 N. Parsons Avenue, Brandon, FL 33510 | Center |
| Mis' Ginny's Inc - Lakewood | 119 S. Lakewood Drive, Brandon, FL 33511 | Center |
| Morning Glory Preschool | 6001 Christy Lane, Riverview, FL 33578 | Preschool |
| Nativity Catholic School | 705 E. Brandon Boulevard, Brandon, FL 33511 | Center |
| Post Sunshine Ranch | 1350 John Moore Road, Brandon, FL 33511 | Preschool |
| Preschool Academy I | 6130 Florence Avenue, Gibsonton, FL 33534 | Preschool |
| Progress Village Academy | 8616 Progress Boulevard, Tampa, FL 33619 | Center |
| Providence Christian School Preschool | 5416 Providence Road, Riverview, FL 33569 | Center |
| RCMA Balm Child Development Center | 14710 Sweet Charlie Circle, Wimauma, FL 33598 | Preschool |
| RCMA Dover Child Development Center | 3238 San Jose Mission Drive, Dover, FL 33527 | Preschool |
| Resurrection Christian School Preschool | 3225 Bell Shoals Road, Brandon, FL 33511 | Center |
| Resurrection Early Childhood Center | 6819 Krycul Avenue, Riverview, FL 33578 | Center |
| Riverview Children's Academy | 10602 County Road 672, Riverview, FL 33579 | Center |
| Seminole Heights United Methodist Preschool | 509 E. Hanna Street, Brandon, FL 33510 | Preschool |
| Shining Stars Preschool Academy | 603 E. Morgan Street, Brandon, FL 33510 | Preschool |
| Simmon's Achievement Center | 107 E. Clay Avenue, Brandon, FL 33510 | Preschool |
| South Shore Learning Center | 11525 Big Bend Road, Riverview, FL 33569 | Preschool |
| St. Andrew's United Methodist Preschool | 3315 S. Bryan Road, Brandon, FL 33511 | Preschool |
| St. Stephens Catholic School | 10424 Saint Stephen Circle, Riverview, FL 33569 | Center |
| Staci's Learning Station | 10611 Cone Grove Road, Riverview, FL 33578 | Center |
| Sunshine Academy | 7739 Gibsonton Drive, Gibsonton, FL 33534 | Preschool |
| Tambra's Tiny Steps Learning Academy II | 4922 N. 84th Street, Tampa, FL 33619 | Preschool |
| Temple Christian Academy | 2909 John Moore Road, Brandon, FL 33511 | Center |
| The Brandon Montessori School at River Hills | 4223 Lynx Paw Trail, Valrico, FL 33596 | Preschool |