| U.S. Kids Child Development Center | 1425 New York Ave Nw, Washington, DC 20005 | Center |
| Ujamaa School | 1554 8th St Nw, Washington, DC 20001 | Center |
| United Community Early Learning Center | 2709 Popkins Lane, Alexandria, VA 22306 | Center |
| University for Tots | 2403 Whitehall Street, Suitland, MD 20746 | Preschool |
| Upo #8 Juanita Thornton Early Child Development Center | 625 H St Ne, Washington, DC 20002 | Center |
| Upo - Early Child Development Center - Atlantic Terrace Child Development Center | 4309 3rd St Se, Washington, DC 20032 | Center |
| Upo - Randall Day Care Center | 900 I Street, Sw, Washington, DC 20024 | Center |
| Usda - Children's Discovery Center | 201 14th St Sw, Washington, DC 20250 | Center |
| Valley Drive Preschool | 700 Commonwealth Avenue, Alexandria, VA 22301 | Preschool |
| Vee's Child Development Center | 2130 Minnesota Ave Se, Washington, DC 20020 | Center |
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| Veronica L. Morris | 1739 D St, Ne, Washington, DC 20002 | Center |
| Waterfront Child Development Center | 1545 1st St, Sw, Washington, DC 20024 | Center |
| Wendy Weekes - Little Apple Child Center | 908 Southern Ave, Se, Washington, DC 20032 | Center |
| Westminster School After-School Care Program and Summer Fun Camp | 3819 Gallows Road, Annandale, VA 22003 | Preschool |
| Westminster Weekday Preschool | 2701 Cameron Mills Road, Alexandria, VA 22302 | Preschool |
| Wibble Wobble Child Development Center | 1 Randle Cir Se, Washington, DC 20019 | Center |
| Wilmer, Cutler & Pickering Child Development Center | 1899 Pennsylvania Ave Nw, Washington, DC 20037 | Center |
| Woodridge Day Care Center II | 68 Forrester St, Sw, Washington, DC 20032 | Center |
| Ximena Cespedes | 1329 North Carolina Ave, Ne, Washington, DC 20002 | Center |
| YMCA Anthony Bowen Child Development Center - Mount Airy | 1100 North Capitol St, Nw, Washington, DC 20002 | Center |
| YMCA METRO - Alexandria | 420 East Monroe Avenue, Alexandria, VA 22301 | Preschool |
| YMCA METRO - Arlington Branch | 3422 North 13th Street, Arlington, VA 22201 | Preschool |
| YMCA METRO - Arlington United Methodist Church | 716 South Glebe Road, Arlington, VA 22204 | Preschool |
| YMCA Metro - Woodbury Park | 2230 N. Fairfax Drive, Arlington, VA 22201 | Preschool |
| YMCA Potomac Overlook | 10709 Indian Head Highway, Fort Washington, MD 20744 | Preschool |
| Young Stars Early Learning Center | 5004 McKinley Street, Temple Hills, MD 20748 | Preschool |
| Young's Memorial Church Day Care | 2490 Alabama Ave Se, Washington, DC 20020 | Center |
| Zena's Child Development Center #1 | 4119 4th St Se, Washington, DC 20032 | Center |
| Zenas Child Development Center | 109 Wayne Pl, Se, Washington, DC 20032 | Center |