| Aloma Methodist Early Childhood Learning Center | 3045 Aloma Avenue, Winter Park, Florida, Winter Park, FL 32792 | Preschool |
| Childtime of Winter Park | 1001 South Semoran Boulevard, Winter Park, FL 32792 | Preschool |
| Dorscher KinderCare | 644 Dorscher Rd, Orlando, FL 32818 | Preschool |
| Goldenrod Road KinderCare | 4035 N Goldenrod Rd, Winter Park, FL 32792 | Preschool |
| La Petite Academy of Altamonte Springs | 500 SR 434, Altamonte Springs, FL 32714 | Preschool |
| La Petite Academy of Lake Mary | 3850 Lake Emma Rd, Lake Mary, FL 32746 | Preschool |
| La Petite Academy of Ocoee | 11002 Winshire Blvd, Ocoee, FL 34761 | Center |
| La Petite Academy of Orlando | 6573 Old Winter Garden Rd, Orlando, FL 32811 | Preschool |
| La Petite Academy of Orlando | 7698 Silver Star Road, Orlando, FL 32818 | Preschool |
| La Petite Academy of Orlando | 2650 Pembrook Drive, Orlando, FL 32810 | Preschool |
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| Longwood KinderCare | 2654 W State Road 434, Longwood, FL 32779 | Preschool |
| Winter Springs KinderCare | 1024 Spring Villas Pt, Winter Springs, FL 32708 | Preschool |
| 4 Kidz | 7641 Magnolia Homes Road, Orlando, FL 32810 | Center |
| 4-C Seminole Head Start Altamonte Springs | 525 Pineview St, Altamonte Springs, FL 32701 | Center |
| 4C Head Start - North Street | 900 North Street, Longwood, FL 32750 | Preschool |
| A Child's Discovery Center | 211 South Hunt Club Boulevard, Apopka, FL 32703 | Preschool |
| A Child's Place | 2301 E Michigan St, Orlando, FL 32806 | Preschool |
| A Place 4 Tots | 1427 N Pine Hills Road, Orlando, FL 32808 | Preschool |
| A Tot's World II | 900 Silkwood Ct, Longwood, FL 32750 | Preschool |
| A Tot's World III | 480 West State Road 434, Winter Springs, FL 32708 | Preschool |
| ABC Learning Center and Child Care | 2310 East Concord Street, Orlando, FL 32803 | Preschool |
| ABC Learning Tree | 2778 South Westmoreland Drive, Orlando, FL 32805 | Preschool |
| Academy 2000 | 925 South Ivey Lane, Orlando, FL 32811 | Center |
| Academy 2000 | 624 Bethune Drive, Orlando, FL 32805 | Preschool |
| Ace Gymnastics dba All Children Excel Academy | 400 Gold Medal Ct, Longwood, FL 32750 | Preschool |
| Adorable Treasures Academy | 4750 Silver Star Road, Orlando, FL 32808 | Center |
| Adorable Treasures Pre-School Academy | 4801 Basswood Lane, Orlando, FL 32808 | Preschool |
| Adorable Treasures Preschool | 4750 Silver Star Road, Orlando, FL 32808 | Preschool |
| Advanced Beginnings Childcare Development | 406 E. Amelia Street, Orlando, FL 32803 | Preschool |
| Adventure Preschool and Daycare | 4652 South Orange Blossom Trail, Orlando, FL 32839 | Preschool |
| Agape Christian Academy and Preschool | 2425 North Hiawassee Road, Orlando, FL 32818 | Center |
| All God's Angels Preschool & Child Care | 1215 Mercy Drive, Orlando, FL 32808 | Preschool |
| All Saints School | 338 East Lyman Avenue, Winter Park, FL 32789 | Preschool |
| All Stars Childcare Center | 7307 West Colonial Drive Suite 7429, Orlando, FL 32818 | Preschool |
| Aloma Elementary School Preschool | 2949 Scarlet Road, Winter Park, FL 32789 | Preschool |
| Aloma Kids Academy | 3416 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Aloma Kids of Orlando | 3416 Aloma Ave, Winter Park, FL 32792 | Preschool |
| Alphabet Gang Child Care | 3615 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Altamonte Elementary Pre-k | 525 Pineview Street, Altamonte Springs, FL 32701 | Preschool |
| Altamonte Kiddie Cottage | 290 Maitland Ave, Altamonte Springs, FL 32701 | Preschool |
| Altamonte Learning Center | 1329 Altamonte Drive, Casselberry, FL 32707 | Preschool |
| Altamonte Montessori School | 482 Osceola Street, Altamonte Springs, FL 32701 | Preschool |
| American Village Academy | 905 Longwood Hills Road, Longwood, FL 32750 | Preschool |
| Anchor Academy | 23 North Wekiva Springs Road, Apopka, FL 32703 | Preschool |
| Angel Sprouts Academy | 7651 Valencia College Lane, Orlando, FL 32807 | Preschool |
| Annunciation Child Care Center | 970 Montgomery Road, Altamonte Springs, FL 32714 | Preschool |
| Anointed Word Faith Academy | 305 S Highland Avenue, Apopka, FL 32703 | Preschool |
| Apopka Child Development Center | 170 East Magnolia Street, Apopka, FL 32703 | Preschool |
| Apostolic Worship Child Development Center | 8001 Silver Star Road, Orlando, FL 32818 | Preschool |
| Arcoiris De Amor Child Care | 929 W. Michigan Street, Orlando, FL 32805 | Center |
| Arising Little Angels Preschool | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Arising Little Angels Preschool Academy | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Ascension Lutheran Early Childhood Program | 351 Ascension Drive, Casselberry, FL 32707 | Preschool |
| Audubon Elementary School Preschool | 1750 Common Way Rd, Orlando, FL 32814 | Preschool |
| Augustina Academy | 1307 Pine Hills Road, Orlando, FL 32808 | Preschool |
| Azalea Park Baptist School | 5725 Dahlia Dr, Orlando, FL 32807 | Preschool |
| Azalea Park Elementary School Preschool | 1 Carol Avenue, Orlando, FL 32807 | Preschool |
| Azalea Park Learning Center | 940 South Semoran Boulevard, Orlando, FL 32807 | Preschool |
| BETA Center Developmental Childcare | 4680 Lake Underhill Rd, Orlando, FL 32807 | Preschool |
| Baby Days Infant Care | 109 S Pressview Ave, Longwood, FL 32750 | Center |
| Baby Go Play! | 417 Center Pointe Circle Suite 1701, Altamonte Springs, FL 32701 | Center |
| Baldwin Oaks Academy | 1862 Winter Park Road, Orlando, FL 32803 | Center |
| Bays Childcare Center | 1239 North Pine Hills Road, Orlando, FL 32808 | Center |
| Bear Lake Elementary Preschool | 3399 Gleaves Court, Apopka, FL 32703 | Preschool |
| Beginning Years Learning Center | 301 West Welch Road, Apopka, FL 32712 | Preschool |
| Beryl Wisdom Adventist School | 4955 Rose Ave., Orlando, FL 32808 | Preschool |
| Best Memories Academy Child Care Center | 5135 Curry Ford Road, Orlando, FL 32812 | Preschool |
| Big Children's Academy | 3300 S. Conway Road, Orlando, FL 32812 | Preschool |
| Bright Horizons at Baldwin Park | 1700 Fire House Lane, Orlando, FL 32814 | Preschool |
| Bright Stars Preschool | 7325 Conroy Windermere Road, Orlando, FL 32835 | Preschool |
| Brighter Futures Enrichment & Learning Center | 221 East Kennedy Boulevard, Maitland, FL 32751 | Preschool |
| Brightfutures Academy | 1500 Shepard Road, Winter Springs, FL 32708 | Preschool |
| Brush Arbor Christian | 2304 North Goldenrod Road, Orlando, FL 32807 | Center |
| Bubbles Pond Day Care | 3423 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| CHS A Place For Children | 425 North Orange Avenue R270 S ..., Orlando, FL 32801 | Center |
| CSA Preschool | 645 Executive Park Court, Apopka, FL 32703 | Preschool |
| Callahan Headstart | 101 North Parramore Avenue, Orlando, FL 32801 | Preschool |
| Casselberry Elementary Vpk - Seminole Co | 1075 Crystal Bowl Circle, Casselberry, FL 32707 | Preschool |
| Catalina Elementary School Preschool | 2448 W. 29th Street, Orlando, FL 32805 | Preschool |
| Center For Early Childhood Education | 341 North Orlando Avenue, Maitland, FL 32751 | Preschool |
| Cheney Elementary School Preschool | 2000 N. Forsyth Road, Orlando, FL 32807 | Preschool |
| Chicksaw Elementary School Preschool | 6900 Autumnvale Dr., Orlando, FL 32822 | Preschool |
| Childlife Preschool | 5495 Lake Margaret Drive, Orlando, FL 32812 | Preschool |
| Children Palace Learning Center | 1230 North Pine Hills Rd, Orlando, FL 32808 | Preschool |
| Children's Academy at Loch Haven | 200 E Evans St, Orlando, FL 32804 | Preschool |
| Children's Academy at Loch Haven - Mildly Ill | 227 East Evans Street, Orlando, FL 32804 | Preschool |
| Children's Destiny Learning Center | 724 N Hastings Street, Orlando, FL 32818 | Center |
| Children's Learning Academy | 2333 E. Semoran Blvd, Apopka, FL 32703 | Preschool |
| Children's Palace | 1800 W Washington St, Orlando, FL 32805 | Center |
| Choices In Learning Charter School Preschool | 893 E. State Road 434, Longwood, FL 32750 | Preschool |
| Christian Child Center | 211 Wekiva Springs Lane, Longwood, FL 32779 | Preschool |
| Chuck and Kerry's House | 234 Concord Drive, Casselberry, FL 32707 | Preschool |
| Circle Time Child Care | 2750 N Hiawassee Road, Orlando, FL 32818 | Preschool |
| Clarcona Academy Child Care | 4300 Clarcona Ocoee Rd, Orlando, FL 32810 | Preschool |
| Coalition For The Homeless | 639 W Central Blvd, Orlando, FL 32801 | Preschool |
| College Park Kiddie Kampus | 2208 N Rio Grande Ave, Orlando, FL 32804 | Center |
| College Park U M Child Dev Center | 44 West Princeton Street, Orlando, FL 32804 | Preschool |
| Columbia Elementary School Preschool | 18501 Cypress Lake Glen, Orlando, FL 32801 | Preschool |
| Columbus Center | 4629 Middlebrook Road, Orlando, FL 32811 | Preschool |
| Community Alliance Preschool | 4815 E Lake Dr, Winter Springs, FL 32708 | Preschool |