| Alaqua Lakes KinderCare | 2635 W Lake Mary Blvd, Lake Mary, FL 32746 | Center |
| International Parkway KinderCare | 3050 International Pkwy, Lake Mary, FL 32746 | Center |
| La Petite Academy of Altamonte Springs | 500 SR 434, Altamonte Springs, FL 32714 | Preschool |
| La Petite Academy of Deltona | 698 Deltona Blvd, Deltona, FL 32725 | Preschool |
| La Petite Academy of Lake Mary | 3850 Lake Emma Rd, Lake Mary, FL 32746 | Preschool |
| La Petite Academy of Orlando | 2650 Pembrook Drive, Orlando, FL 32810 | Preschool |
| Longwood KinderCare | 2654 W State Road 434, Longwood, FL 32779 | Preschool |
| Winter Springs KinderCare | 1024 Spring Villas Pt, Winter Springs, FL 32708 | Preschool |
| 4-C Headstart - Midway | 2255 Rightway Street, Sanford, FL 32771 | Center |
| 4-C Seminole Head Start Altamonte Springs | 525 Pineview St, Altamonte Springs, FL 32701 | Center |
|
| 4C Head Start - North Street | 900 North Street, Longwood, FL 32750 | Preschool |
| 4C Seminole Head Start - Hamilton | 1414 Celery Ave, Sanford, FL 32773 | Preschool |
| 4C Seminole Head Start Pinecrest | 405 W 27th Street, Sanford, FL 32773 | Center |
| A Child's Discovery Center | 211 South Hunt Club Boulevard, Apopka, FL 32703 | Preschool |
| A Tot's World II | 900 Silkwood Ct, Longwood, FL 32750 | Preschool |
| A Tot's World III | 480 West State Road 434, Winter Springs, FL 32708 | Preschool |
| Academic Beginnings Child Care | 315 Maple Avenue, Sanford, FL 32771 | Preschool |
| Ace Gymnastics dba All Children Excel Academy | 400 Gold Medal Ct, Longwood, FL 32750 | Preschool |
| All God's Children Preschool | 240 San Marcos Avenue, Sanford, FL 32771 | Center |
| Altamonte Elementary Pre-k | 525 Pineview Street, Altamonte Springs, FL 32701 | Preschool |
| Altamonte Kiddie Cottage | 290 Maitland Ave, Altamonte Springs, FL 32701 | Preschool |
| Altamonte Learning Center | 1329 Altamonte Drive, Casselberry, FL 32707 | Preschool |
| Altamonte Montessori School | 482 Osceola Street, Altamonte Springs, FL 32701 | Preschool |
| American Village Academy | 905 Longwood Hills Road, Longwood, FL 32750 | Preschool |
| Annunciation Child Care Center | 970 Montgomery Road, Altamonte Springs, FL 32714 | Preschool |
| Ascension Lutheran Early Childhood Program | 351 Ascension Drive, Casselberry, FL 32707 | Preschool |
| Baby Days Infant Care | 109 S Pressview Ave, Longwood, FL 32750 | Center |
| Baby Go Play! | 417 Center Pointe Circle Suite 1701, Altamonte Springs, FL 32701 | Center |
| Bear Lake Elementary Preschool | 3399 Gleaves Court, Apopka, FL 32703 | Preschool |
| Bee Kids Academy | 2581 S. Sanford Avenue, Sanford, FL 32771 | Preschool |
| Bee Kids Academy Too | 1104 West 3rd Street, Sanford, FL 32771 | Center |
| Brighter Futures Enrichment & Learning Center | 221 East Kennedy Boulevard, Maitland, FL 32751 | Preschool |
| Brightfutures Academy | 1500 Shepard Road, Winter Springs, FL 32708 | Preschool |
| Brilliant Minds Montessori | 153 Bellagio Circle, Sanford, FL 32771 | Center |
| CSA Preschool | 645 Executive Park Court, Apopka, FL 32703 | Preschool |
| Cambridge Prep School | 2720 W Lake Mary Blvd, Lake Mary, FL 32746 | Preschool |
| Casselberry Elementary Vpk - Seminole Co | 1075 Crystal Bowl Circle, Casselberry, FL 32707 | Preschool |
| Center For Early Childhood Education | 341 North Orlando Avenue, Maitland, FL 32751 | Preschool |
| Children's Learning Academy | 2333 E. Semoran Blvd, Apopka, FL 32703 | Preschool |
| Choices In Learning Charter School Preschool | 893 E. State Road 434, Longwood, FL 32750 | Preschool |
| Christian Care for Lil' Angels | 74 S Charles R Beall Blvd, Debary, FL 32713 | Preschool |
| Christian Child Center | 211 Wekiva Springs Lane, Longwood, FL 32779 | Preschool |
| Chuck and Kerry's House | 234 Concord Drive, Casselberry, FL 32707 | Preschool |
| City Kidz Academy Preschool | 650 E Airport Blvd, Sanford, FL 32773 | Preschool |
| Community Alliance Preschool | 4815 E Lake Dr, Winter Springs, FL 32708 | Preschool |
| Community Christian Preschool | 4921 South U.s. Highway 17-92, Casselberry, FL 32707 | Preschool |
| Community United Methodist Preschool | 41 W. Highbanks Road, Debary, FL 32713 | Preschool |
| Coqui Academy | 2401 South Park Avenue, Sanford, FL 32771 | Preschool |
| Coqui Academy II | 919 Orange Avenue East, Longwood, FL 32750 | Preschool |
| Crystal Lake Elementary Preschool | 231 Rinehart Road, Lake Mary, FL 32746 | Preschool |
| DeBary Sonshine Academy | 76 W. Highbanks Road, Debary, FL 32713 | Center |
| DeBary Sonshine Afterschool Program | 88 Highbanks Road, Debary, FL 32713 | Center |
| DebaryFirst Presbyterian Preschool | 267 E. Highbanks Road, Debary, FL 32713 | Preschool |
| Denton Johnson Head Start | 400 Ruffle Street, Maitland, FL 32751 | Preschool |
| Divine Individuals Developmental Center | 140 South West Avenue, Maitland, FL 32751 | Preschool |
| Dr Sharon Riggins Patterson Child Development Center | 2400 Chase Avenue, Sanford, FL 32771 | Preschool |
| Early Childhood Academy | 1231 North Brassie Drive, Winter Springs, FL 32708 | Preschool |
| Early Learning Childcare | 159 E Wilbur Ave, Lake Mary, FL 32746 | Preschool |
| Early Start Learning Academy | 2322 East Semoran Blvd, Apopka, FL 32703 | Preschool |
| Einstein Academy | 1030 N. Ronald Reagan Blvd. #300a, Longwood, FL 32750 | Preschool |
| English Estates Pre-k | 299 Oxford Road, Casselberry, FL 32730 | Preschool |
| First Baptist Sweetwater Mother's Day Out | 3800 Wekiva Springs Road, Sanford, FL 32771 | Preschool |
| First Steps of Learning Camp Seminole | 390 Piney Ridge Road, Casselberry, FL 32707 | Preschool |
| First United Methodist Preschool & Childcare Center | 419 Park Avenue, Sanford, FL 32771 | Preschool |
| Florida United Methodist Children's Home Child Care Center | 51 Children's Way, Deltona, FL 32725 | Preschool |
| Forest City Elementary V P K - Seminole | 1010 Sand Lake Road, Lake Mary, FL 32746 | Preschool |
| Forest Lake Education Center | 1275 Learning Loop, Longwood, FL 32779 | Preschool |
| Friends Preschool Academy | 327 Wilma Street, Longwood, FL 32750 | Preschool |
| Genesis Christian Preschool | 1255 E.e. Williamson Road, Longwood, FL 32750 | Preschool |
| Go Play! | 482 East Altamonte Drive Suite 1009, Altamonte Springs, FL 32701 | Preschool |
| Grace United Methodist Church Preschool | 499 North Country Club Road, Lake Mary, FL 32746 | Preschool |
| GraceKids Academy | 1767 W. S.r. 434, Longwood, FL 32750 | Preschool |
| Growing Tree Learning Center | 900 Dodd Road, Casselberry, FL 32707 | Preschool |
| Happy Faces Prep School | 3612 East Mcneil Road, Apopka, FL 32703 | Preschool |
| Heavenly Angelz Kidz Center | 950 Doyle Road, Deltona, FL 32725 | Center |
| Hidden Treasures Pre School and Child Care Center | 1018 West First Street, Sanford, FL 32771 | Preschool |
| Holy Cross Lutheran Child care Center | 5450 Holy Cross Court, Sanford, FL 32771 | Preschool |
| Holy Cross Lutheran Church Preschool | 780 North Sun Drive, Lake Mary, FL 32746 | Preschool |
| Hunt Club Montessori School | 502 Hunt Club Boulevard, Apopka, FL 32703 | Preschool |
| Idyllwilde Elementary Vpk - Seminole County | 430 Vihlen Road, Sanford, FL 32771 | Preschool |
| Ingenuity Preschool | 2505 E. Semoran Blvd, Apopka, FL 32703 | Preschool |
| Ingram's Academy | 7856 Forest City Road, Orlando, FL 32810 | Preschool |
| Jewish Community Center Childcare | 851 North Maitland Avenue, Maitland, FL 32751 | Preschool |
| Kid City USA - Douglas Ave | 841 Douglas Avenue, Altamonte Springs, FL 32714 | Preschool |
| Kid City USA - Lake Mary | 289 Seminole Avenue, Lake Mary, FL 32746 | Preschool |
| Kid City USA - Longwood | 935 Wekiva Springs Rd., Longwood, FL 32779 | Center |
| Kid City USA - Sanford | 2720 W 25th Street, Sanford, FL 32771 | Preschool |
| Kid City USA Winter Park | 3650 Howell Branch Road, Winter Park, FL 32792 | Preschool |
| Kiddy Cats Academy | 360 S Mellonville Ave, Sanford, FL 32771 | Preschool |
| Kids Ark Development Center | 1238 Bunnell Road, Altamonte Springs, FL 32714 | Preschool |
| Kids Castle Child Care Center | 2536 South Elm Avenue, Sanford, FL 32773 | Preschool |
| Kids First Steps | 191 Normandy Road, Casselberry, FL 32707 | Preschool |
| Kids Together of Lake Mary | 756 North Sun Drive, Lake Mary, FL 32746 | Preschool |
| Kidtown USA | 1460 Tuskawilla Road, Winter Springs, FL 32708 | Preschool |
| Kinder Kampus | 640 E Sr 434, Winter Springs, FL 32708 | Preschool |
| KinderCare at Hunt Club - CLOSED | 625 Executive Park Court, Apopka, FL 32703 | Preschool |
| Kinderoo Preschool & Child Care | 389 Spring Oak Blvd, Altamonte Springs, FL 32714 | Preschool |
| La Shundra's Tots Child Care & Development Center | 140 West Street Suite B, Maitland, FL 32751 | Preschool |
| Ladybird Academy | 700 Lake Forest Boulevard, Sanford, FL 32771 | Preschool |
| Ladybird Academy #2 | 185 Timacuan Blvd, Lake Mary, FL 32746 | Preschool |