| Bell Shoals KinderCare | 4304 Bell Shoals Rd, Valrico, FL 33596 | Preschool |
| La Petite Academy of Brandon | 808 W. Bloomingdale Avenue, Brandon, FL 33511 | Preschool |
| La Petite Academy of Brandon | 606 E. Windhorst Road, Brandon, FL 33510 | Preschool |
| La Petite Academy of Riverview | 10025 Cristina Drive, Riverview, FL 33569 | Preschool |
| La Petite Academy of Valrico | 1011 Greenhollow Lane, Valrico, FL 33596 | Center |
| Seffner KinderCare | 2524 S Parsons Ave, Seffner, FL 33584 | Preschool |
| A Bridge of Learning | 3902 Corporex Park Drive, Tampa, FL 33619 | Center |
| A Brighter Community | 1613 N. Marion Street, Tampa, FL 33602 | Preschool |
| A Child's Haven Private Preschool | 1520 Brentwood Hills Boulevard, Valrico, FL 33594 | Preschool |
| A Children's Kastle Early Learning Center | 950 S. Mulrennan Road, Valrico, FL 33594 | Preschool |
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| A Perfect Start Early Learning Childcare Center | 2005 E. 21st Avenue, Tampa, FL 33605 | Center |
| A Plus Child Care Center | 3042 S. 78th Street, Tampa, FL 33619 | Center |
| A Readiness Learning Academy | 489 W. Apollo Beach Boulevard, Apollo Beach, FL 33572 | Preschool |
| A. J.'s Angels II | 1139 Nw. 1st Street, Ruskin, FL 33570 | Center |
| Academy of Achievers | 536 Maydell Drive, Tampa, FL 33619 | Preschool |
| Academy of Active Education | 6716 S. Macdill Avenue, Tampa, FL 33611 | Center |
| Academy of The Holy Names | 3319 Bayshore Boulevard, Tampa, FL 33629 | Center |
| Achievement Center For Early Learning | 5714 S. Dale Mabry Highway, Tampa, FL 33611 | Center |
| All Nations Preschool | 10022 Cristina Drive, Riverview, FL 33569 | Preschool |
| Almand Center | 1311 Brooker Road, Brandon, FL 33511 | Preschool |
| Apollo Beach Christian Preschool | 6414 Golf And Sea Boulevard, Apollo Beach, FL 33572 | Preschool |
| Apostles Lutheran Church Preschool | 200 N. Kingsway Road, Brandon, FL 33510 | Preschool |
| Bayshore Baptist Church Preschool | 3111 W. Morrison Avenue, Tampa, FL 33629 | Preschool |
| Bayshore Christian School | 3909 S. Macdill Avenue, Tampa, FL 33611 | Center |
| Bayside Academy | 1202 E. Shell Point Road, Ruskin, FL 33570 | Center |
| Beach Park Academy II | 3218 W. Bay To Bay Boulevard, Tampa, FL 33629 | Center |
| Bell Shoals Baptist Academy | 2102 Bell Shoals Road, Brandon, FL 33511 | Center |
| Bloomingdale Academy | 11418 Bloomingdale Avenue, Riverview, FL 33578 | Center |
| Brandon Academy | 801 Limona Road, Brandon, FL 33510 | Preschool |
| Brandon Day School | 202 Pauls Drive, Brandon, FL 33511 | Center |
| Brandon Head Start | 9547 E. Columbus Drive, Tampa, FL 33619 | Preschool |
| Brandon Sports and Aquatic Center | 405 Beverly Blvd, Brandon, FL 33511 | Preschool |
| Breakaway Family Learnng Center | 4422 E. Columbus Avenue, Tampa, FL 33605 | Center |
| Brighter Kids | 2207 Durant Road, Valrico, FL 33596 | Center |
| Brookwood Academy | 3820 Coconut Palm Drive, Tampa, FL 33619 | Center |
| Browns Town Daycare | 7739 GIBSONTON DR, Gibsonton, FL 33534 | Center |
| Carlton Academy Day School | 205 N. Brush Street, Tampa, FL 33602 | Preschool |
| Castles of Imagination | 820 Bryan Road, Brandon, FL 33511 | Preschool |
| Central Baptist Christian School | 402 E. Windhorst Road, Brandon, FL 33510 | Center |
| Child-Kare Solutions | 11002 E. Broadway Avenue, Seffner, FL 33584 | Preschool |
| Childcare of Brandon | 730 Brandon Town Center Dr, Brandon, FL 33511 | Center |
| Children With Joy | 2708 N. Central Avenue, Tampa, FL 33602 | Center |
| Children's Academy | 506 Limona Road, Brandon, FL 33510 | Preschool |
| Children's Choice Learning Center | 25 Columbia Drive, Tampa, FL 33606 | Preschool |
| Children's Life Academy | 3120 S. Miller Road, Valrico, FL 33596 | Center |
| Children's Nest Child Care Center | 2601 W. Deleon Street, Tampa, FL 33609 | Preschool |
| Children's Nest Day School | 3221 S. Bryan Road, Brandon, FL 33511 | Preschool |
| Circle C Ranch Academy | 6204 Interbay Boulevard, Tampa, FL 33611 | Preschool |
| Circle M Ranch Day Care | 1202 Bloomingdale Avenue, Valrico, FL 33596 | Preschool |
| Citigroup Family Center | 3701 Queen Palm Drive, Tampa, FL 33610 | Center |
| Colour Their World | 115-a Margaret Street, Brandon, FL 33511 | Preschool |
| Community Christian Child Care | 4215 Orient Road, Tampa, FL 33610 | Preschool |
| Creative World A Child's Adventure | 5931 Frond Way, Apollo Beach, FL 33572 | Preschool |
| Creative World Causeway | 9815 Causeway Boulevard, Tampa, FL 33619 | Preschool |
| Creative World Lil Pals | 1010 S. 76th Street, Tampa, FL 33619 | Preschool |
| Creative World Net Park | 5015 N. 56th Street, Tampa, FL 33610 | Preschool |
| Creative World School Fish Hawk | 5525 Osprey Ridge Drive, Lithia, FL 33547 | Preschool |
| Creative World School at Rivercrest | 11361 Symmes Road, Riverview, FL 33569 | Preschool |
| Dimples Playland Child Care Center - 12th Ave | 7445 S. 12th Avenue, Tampa, FL 33619 | Center |
| Discovery Point #60 | 11719 Symmes Road, Riverview, FL 33569 | Preschool |
| Discovery Point #70 | 13011 Summerfield Crossing Boulevard, Riverview, FL 33579 | Preschool |
| East Bay Christian School | 10102 Old Big Bend Road, Riverview, FL 33578 | Center |
| Faith Baptist Preschool | 1118 North Parsons Avenue, Brandon, FL 33510 | Center |
| First Baptist Brandon Christian Academy | 216 N. Parsons Ave., Brandon, FL 33510 | Center |
| First Discoveries Academy | 1311 Brooker Road, Brandon, FL 33511 | Center |
| First Presbyterian Preschool of Brandon | 121 Carver Street, Brandon, FL 33510 | Preschool |
| First UMChurch Preschool and Kindergarten | 120 N. Knights Avenue, Brandon, FL 33510 | Preschool |
| Fish Hawk Early Learning Center | 5632 Osprey Park Place, Lithia, FL 33547 | Preschool |
| Fish Hawk Early Learning Center II | 6001 Parkset Drive, Lithia, FL 33547 | Preschool |
| Foundation Christian Academy | 3955 Lithia Pinecrest Road, Valrico, FL 33596 | Preschool |
| Good Samaritan Mission | 14920 Balm Wimauma Road, Wimauma, FL 33598 | Preschool |
| Grant Park Head Start | 4422 E. Columbus Drive, Tampa, FL 33605 | Preschool |
| HCPS - Clair-mel Elementary Head Start Vpk | 1025 South 78th Street, Tampa, FL 33619 | Preschool |
| HCPS - Desoto Elem Head Start Vpk | 2618 Corrine Street, Tampa, FL 33605 | Preschool |
| HCPS - Doby Elementary Headstart Vpk | 6720 Covington Garden Drive, Apollo Beach, FL 33572 | Preschool |
| HCPS - Gibsonton Elem Headstart Vpk | 7723 Gibsonton Drive, Gibsonton, FL 33534 | Preschool |
| HCPS - Lanier Elementary Head Start Vpk | 4704 Montgomery Avenue, Tampa, FL 33616 | Preschool |
| HCPS - Oak Park Elementary Headstart Vpk | 2716 North 46th Street, Tampa, FL 33605 | Preschool |
| HCPS - Washington Elementary Headstart Vpk | 1407 East Estelle Street, Tampa, FL 33605 | Preschool |
| HCPS - West Tampa Elementary Headstart Vpk | 2700 Cherry St., Tampa, FL 33619 | Preschool |
| Happy Day Preschool | 722 S. Lithia Pinecrest Road, Brandon, FL 33511 | Preschool |
| Harvest Time Christian School | 1511 S. Us 301, Tampa, FL 33619 | Center |
| Harvest Time Christian School | 1511 U S 301 South, Tampa, FL 33619 | Center |
| Heaven Sent Day Care Center | 3003 S. Kings Avenue, Brandon, FL 33511 | Center |
| Hillsborough Community College Ybor Child Development Center | 1505 Palm Avenue, Tampa, FL 33605 | Preschool |
| Holy Trinity Lutheran School | 3712 W. El Prado Blvd, Tampa, FL 33629 | Preschool |
| Home Away From Home Daycare Center #2 | 7415 Destin Drive, Tampa, FL 33619 | Center |
| Home Away From Home Learning Center | 8516 Ridein Road, Tampa, FL 33619 | Center |
| Horizon Early Learning Preschool | 1720 S. Saint Cloud Avenue, Valrico, FL 33594 | Preschool |
| Humpty Dumpty Academy | 1211 Wishing Well Way, Tampa, FL 33619 | Center |
| Hyde Park Day School | 909 Horatio Street, Tampa, FL 33606 | Center |
| Immanuel Lutheran Pre-school | 2913 John Moore Road, Brandon, FL 33511 | Preschool |
| It's Kidz Time Of Riverview | 11329 Big Bend Road, Riverview, FL 33569 | Center |
| Jewish Community Center Preschool South Branch | 2710 S. Ysabella Avenue, Tampa, FL 33629 | Preschool |
| Kid's Community College - Lake St. Charles | 10544 Lake St. Charles Blvd, Riverview, FL 33578 | Preschool |
| Kiddie Kollege Baby World | 4350 S. Manhattan Avenue, Tampa, FL 33611 | Center |
| Kiddie Kollege Day Care Center | 4319 W. Fair Oaks Avenue, Tampa, FL 33611 | Center |
| Kids R Kids #8 FL | 4321 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| Kids R Kids Southshore | 13151 Kings Lake Drive, Gibsonton, FL 33534 | Preschool |
| Kids R Tops | 215 N. Kingsway Road, Brandon, FL 33510 | Center |