| La Petite Academy of Prospect | 9505 U.S. Highway 42, Prospect, KY 40059 | Preschool |
| Zorn KinderCare | 730 Zorn Ave, Louisville, KY 40206 | Preschool |
| 41st Street Family Care Center | 132 South 41st Street, Louisville, KY 40212 | Preschool |
| A Alphabet Academy Child Care Center | 1803 West Broadway Avenue, Louisville, KY 40203 | Preschool |
| A Kid's World | 3215 Ballard Lane, New Albany, IN 47150 | Center |
| A Kids World | 3416 Hamburg Pike, Jeffersonville, IN 47130 | Center |
| A New Dawn Daycare | 302 Hunter Station Rd., Sellersburg, IN 47172 | Preschool |
| ABC Village Daycare | 1801 West Market Street, Louisville, KY 40212 | Preschool |
| Angels of Myricles | 1225 West Jefferson Street, Louisville, KY 40203 | Preschool |
| Arms of Love Child Care Center | 520 North 26th Street, Louisville, KY 40212 | Preschool |
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| Bobbie's III | 219 South Preston Street, Louisville, KY 40202 | Preschool |
| Breckenridge Franklin Elementary Childcare Enrichment Program | 1351 Payne St., Louisville, KY 40206 | Center |
| Bright Horizons Child Care & Early Education | 632 South Fourth Avenue, Louisville, KY 40202 | Preschool |
| Brown School Childcare Enrichment Program | 546 South First Street, Louisville, KY 40202 | Center |
| Brownsboro Road Childcare - Learning Center | 2407 Brownsboro Road, Louisville, KY 40206 | Preschool |
| Bullfrogs & Butterflies Enrichment Center | 3935 Bank Street, Louisville, KY 40212 | Preschool |
| Butchertown S.J. Catholic Preschool | 114 Adams Street, Louisville, KY 40206 | Preschool |
| C.A.S.I. Head Start Charlestown Center | 214 McCampbell Street, Charlestown, IN 47111 | Preschool |
| CASI Head Start - Eastlawn Center | 1613 East Eighth Street, Jeffersonville, IN 47130 | Preschool |
| Chenoweth Elementary Childcare Enrichment Program | 3622 Brownsboro Road, Louisville, KY 40207 | Center |
| Chestnut Street YMCA Child Development | 930 West Chestnut Street, Louisville, KY 40203 | Preschool |
| Child Care Partners | 619 Wall Street, Jeffersonville, IN 47130 | Center |
| Children's Choice Learning Centers | 600 Martin Luther King #122, Louisville, KY 40202 | Preschool |
| Christ Church Preschool and Kindergarten | 4614 Brownsboro Road, Louisville, KY 40207 | Preschool |
| Coleridge-Taylor Elementary Childcare Enrichment Program | 1130 West Chestnut Street, Louisville, KY 40203 | Center |
| Colgate Baptist Day Care Ministry | 125 East Montgomery Avenue, Clarksville, IN 47129 | Center |
| Come & Grow Childcare Ministry | 203 Cherry Street, New Albany, IN 47150 | Center |
| Covenant Life Childcare Ministry | 2326 Veterans Parkway, Jeffersonville, IN 47130 | Center |
| Crescent Hill Child Care | 2530 Frankfort Avenue, Louisville, KY 40206 | Preschool |
| Cuddles 2 Cuddles #2 | 508 South 18th Street, Louisville, KY 40211 | Preschool |
| Cuddles 2 Cuddles Child Learning Center | 3129 W Muhammad Ali, Louisville, KY 40212 | Preschool |
| Dare to Dream Child Enrichment Center | 236 Amy Avenue, Louisville, KY 40212 | Preschool |
| Discovery Time II | 4010 Discovery Court, New Albany, IN 47150 | Center |
| Discovery Time II | 4000 Discovery Court, New Albany, IN 47150 | Center |
| Dunn Elementary Childcare Enrichment Program | 2010 Rudy Lane, Louisville, KY 40211 | Center |
| First Kids Early Childhood & School Age Care | 7700 U.S. Highway 42, Louisville, KY 40241 | Preschool |
| First Kids Early Childhood and School Age Care | 7700 U.S. Highway 42, Louisville, KY 40241 | Center |
| Floyd County Head Start | 1902 Corydon Pike, New Albany, IN 47150 | Preschool |
| Future Leaders Child Development Center | 2331 West Madison Street, Louisville, KY 40211 | Preschool |
| God's Kids Learning Academy | 8361 Old Hwy. 31 North, Morris, AL 35116 | Center |
| Goshen Elementary at Hillcrest SWAMP After School Enrichment Program | 12518 Ridgemoor Drive, Prospect, KY 40059 | Center |
| Graceland Day School | 3600 Kamer-Miller Road, New Albany, IN 47150 | Center |
| Graceland Day School #2 | 3600-B Kamer-Miller Road, New Albany, IN 47150 | Center |
| Harmony Elementary Youth Enrichment Program | 1901 South Highway 1793, Goshen, KY 40026 | Preschool |
| Heartland Hugs | Two Heartland Way, Jeffersonville, IN 47130 | Center |
| Holy Family Childcare Ministry | 217 West Daisy Lane, New Albany, IN 47150 | Center |
| Image of God Child Care Ministry | 3106 Middle Road, Jeffersonville, IN 47130 | Center |
| J-Town Pre-School | 3922 Ruckriegel Parkway, Suite 214, Louisville, KY 40299 | Preschool |
| Jefferson Community and Technical College Early Childhood Development Center | 800 West Chestnut Street, Louisville, KY 40203 | Preschool |
| Jellybean Clubhouse Day Care Center | 210 South 24th Street, Louisville, KY 40212 | Preschool |
| John Paul II Academy After School Care - Early Childhood Program | 3525 & 3521goldsmith Lane, Louisville, KY 40220 | Preschool |
| Joyful Noise Child Care of Galatian Missionary Baptist Church | 2020 Maryland Avenue, New Albany, IN 47150 | Center |
| Kid Marx Christian Academy | 1401 Mitchell Avenue, Jeffersonville, IN 47130 | Preschool |
| Kid's Connection Child Care Center and Preschool | 1207 Bridgeport Drive, Jeffersonville, IN 47130 | Preschool |
| Kid's Connection Child Care Center and Preschool | 805 South Indiana Avenue, Sellersburg, IN 47172 | Preschool |
| Kid's Paradise Enrichment Center | 3400 Vermont Avenue, Louisville, KY 40211 | Preschool |
| Kids First | 2780 Charlestown Road, New Albany, IN 47150 | Center |
| Kids First Academy III | 529 North 22nd Street, Louisville, KY 40203 | Preschool |
| Kids Kozy Korner III | 2133 W. Market Street, Louisville, KY 40210 | Preschool |
| Kids Palace | 1615 Grant Line Road, New Albany, IN 47150 | Center |
| Kids R Us II | 3400 River Park Drive, Louisville, KY 40211 | Preschool |
| Kids-N-More | 221 South Shelby Street, Louisville, KY 40206 | Preschool |
| Kings Kids Child Care Ministry | 1501 Thomas V. Bryant, Jeffersonville, IN 47130 | Center |
| Learning Blocks Pre School Academy | 1613 E. 8th Street Rm. 13, Jeffersonville, IN 47130 | Center |
| Lil' Genius Child Care Center | 1121 West Broadway, Louisville, KY 40203 | Preschool |
| Little Blessings Day Care | 105 Warren Avenue, Louisville, MS 39339 | Center |
| Little Honey's Learning Center | 3509 West Market Street, Louisville, KY 40211 | Preschool |
| Little Lambs Childcare Development Center 2 | 370 North 26th Street, Louisville, KY 40212 | Preschool |
| Little Robins Childcare Ministry | 6710 Wolf Pen Branch Road, Harrods Creek, KY 40027 | Preschool |
| Love and Prayer Childcare | 1933 West Madison Street, Louisville, KY 40203 | Preschool |
| Malysz Norwood Daycare | 1707 Klerner Lane, New Albany, IN 47150 | Center |
| Mini Miracles Child Enrichment Center | 2933 West Muhammad Ali Blvd., Louisville, KY 40212 | Preschool |
| Mini-Versity Downtown Child Development Center | 306 Roy Wilkins Avenue, Louisville, KY 40203 | Preschool |
| Mini-Versity West Child Development Center | 631 South 28th Street, Louisville, KY 40211 | Preschool |
| Mount Tabor Wee Care | 918 Mount Tabor Road, New Albany, IN 47150 | Center |
| Nee-Nee's Angels Daycare II | 400 South 41st Street, Louisville, KY 40212 | Preschool |
| Neighborhood House Preschool | 201 North 25th Street, Louisville, KY 40212 | Preschool |
| OLPH Daycare Ministry | 1752 Scheller Lane, New Albany, IN 47151 | Center |
| Park Place Methodist Kids Care | 1820 East Park Place, Jeffersonville, IN 47130 | Center |
| Paytons Funland Childcare | 840 East Chestnut Street, Louisville, KY 40202 | Preschool |
| Promise Academy | 516 West Main Street, New Albany, IN 47150 | Preschool |
| Prospect Latin School | 8907 Us Hwy 42, Prospect, KY 40059 | Preschool |
| Prospect Preschool Academy | 13001 West Us 42, Prospect, KY 40059 | Preschool |
| Rise & Shine Daycare Center | 207 South Hancock, Louisville, KY 40202 | Preschool |
| Sacred Heart Extended Care & Preschool | 1842 East Eighth Street, Jeffersonville, IN 47130 | Preschool |
| Second Presbyterian Weekday School | 3701 Old Brownsboro Road, Louisville, KY 40207 | Preschool |
| Seeds of Knowledge Preschool and Day Care Ministry | 3838 Paoli Pike, Floyds Knobs, IN 47119 | Preschool |
| Sellersburg Center | 490 N Indiana, Sellersburg, IN 47172 | Preschool |
| Southside Christian Child Care #14 | 920 Springdale Dr, Jeffersonville, IN 47130 | Preschool |
| Southside Christian Child Care Center #16 | 157 Quartermaster Court, Jeffersonville, IN 47130 | Preschool |
| Springdale Preschool | 7812 Old Brownsboro Road, Louisville, KY 40241 | Preschool |
| St. Francis Preschool | 6710 Wolf Pen Branch Road, Harrods Creek, KY 40027 | Preschool |
| St. John Child Care | 12700 West Highway 42, Prospect, KY 40059 | Preschool |
| St. Joseph Child Development Center | 2823 Frankfort Avenue, Louisville, KY 40206 | Preschool |
| St. Leonard Preschool | 440 Zorn Avenue, Louisville, KY 40206 | Preschool |
| St. Mary of the Knobs Child Care Ministry | 3033 Martin Road, Floyds Knobs, IN 47119 | Preschool |
| St. Michael Child Care | 102 St. Michael Drive, Charlestown, IN 47111 | Center |
| St. Michael Community Childcare Ministry #2 | 102-B St. Michael Drive, Charlestown, IN 47111 | Center |
| St. Paul Child Care Ministry | 216 Schellers Avenue, Sellersburg, IN 47172 | Preschool |
| St. Paul Child Care Ministry | 218 Schellers Avenue, Sellersburg, IN 47172 | Preschool |