| APPLE Academy of Central Presbyterian Church | 301 Euclid Avenue, Bristol, VA 24201 | Preschool |
| Access Early Childhood Services | 478 Valley Street -willimantic, Bristol, NH 03222 | Preschool |
| Anderson Elementary - Leaps | 901 9th Street, Bristol, TN 37620 | Center |
| Anderson Preschool | 901 Ninth Street, Bristol, TN 37620 | Preschool |
| Avoca Elementary - Leaps | 2440 Volunteer Pkwy, Bristol, TN 37620 | Center |
| Avoca Preschool | 2440 Volunteer Parkway, Bristol, TN 37620 | Preschool |
| Bluff City Elementary Pre-kindergarten | 282 J. Forrest Thomas Street, Bluff City, TN 37618 | Preschool |
| Boys & Girls Club of Bristol - Virginia Unit | 334 Rebecca Street, Bristol, VA 24201 | Center |
| Boys And Girls Club Of Bristol | 701 Florida Ave, Bristol, TN 37620 | Center |
| Bristol Child Development Center | 800 Martin Luther King, Jr. Blvd., Bristol, VA 24201 | Preschool |
|
| Building Blocks | 716 Oakview Avenue, Bristol, VA 24201 | Center |
| By-pass Day Care Center | 105 Hatchall Way, Bristol, TN 37620 | Center |
| Childcare Network #123 | 15 Heritage Drive, Bristol, VA 24201 | Preschool |
| Creative Learning Center | 1 Virginia Street, Bristol, VA 24201 | Center |
| Emmett Preschool | 753 Emmett Road, Bristol, TN 37620 | Preschool |
| Fairmount Elementary - Leaps | 821 Virginia Avenue, Bristol, TN 37620 | Center |
| Fairmount Preschool | 821 Virginia Avenue, Bristol, TN 37620 | Preschool |
| Florida Avenue Head Start | 701 Florida Ave., Bristol, TN 37620 | Preschool |
| Girls Incorporated of Bristol | 613 Highland Avenue, Bristol, VA 24201 | Center |
| Grandma's Place | 509 Bluff City Highway, Bristol, TN 37620 | Center |
| Haynesfield Elementary - Leaps | 201 Bluff City Hwy, Bristol, TN 37620 | Center |
| Haynesfield Preschool | 201 Bluff City Highway, Bristol, TN 37620 | Preschool |
| Holston View Elementary - Leaps | 1840 King College Road, Bristol, TN 37620 | Center |
| Holston View Preschool | 1840 King College Road, Bristol, TN 37620 | Preschool |
| Joy In Learning | 489 Egypt Rd., Bluff City, TN 37618 | Center |
| Lauter Park Day Camp | 625 Jackson Street, Bristol, NH 03222 | Center |
| Little Hands Christian Child Care Center | 1864 Holston Dr., Bristol, TN 37620 | Center |
| Little Kids Country | 187 Hardin Hollow Road, Elizabethton, TN 37643 | Center |
| Littlest Angel Child Care Center | 2417 Volunteer Parkway, Bristol, TN 37620 | Center |
| Northwest Elementary Idea 619 Pre-kindergarten | 344 Woodson Drive, Bristol, TN 37621 | Preschool |
| Nurtury Developmental Child Care and Preschool | 1206 Lawrence Avenue, Bristol, VA 24201 | Center |
| Piney Bluff Childcare | 4429 Bluff City Hwy, Bluff City, TN 37618 | Center |
| Riceville Wins | Highway 11 South, Riceville, TN 37370 | Center |
| Shady Valley Pre-kindergarten | 423 Highway 133, Shady Valley, TN 37688 | Preschool |
| St. Anne Catholic School - Preschool and Afterschool Programs | 300 Euclid Avenue, Bristol, VA 24201 | Preschool |
| Sullins Academy PreSchool and Children's Center | 22218 Sullins Academy Drive, Bristol, VA 24202 | Preschool |
| Tennessee Avenue Baptist Child Care Center | 822 Tennessee Avenue, Bristol, TN 37620 | Center |
| Tn Ave Baptist Child Care - Preschool | 822 Tennessee Avenue, Bristol, TN 37620 | Preschool |
| Unaka Elementary Carter Cares | 120 Unaka Drive, Elizabethton, TN 37643 | Center |
| Valley Pike Elementary Preschool | 2125 Carolina Avenue, Bristol, TN 37620 | Preschool |
| Vance Middle School - Leaps | 815 Edgemont Avenue, Bristol, TN 37620 | Center |
| Victory Children's Center | 11123 Island Road, Bristol, VA 24202 | Center |
| Weaver Head Start | 3341 Weaver Pike, Bristol, TN 37620 | Preschool |
| YWCA - Children's Center | 106 State Street, Bristol, TN 37620 | Center |
| YWCA - Tech Gyrls | 106 State Street, Bristol, TN 37620 | Center |