| La Petite Academy of Knoxville | 9001 George Williams Road, Knoxville, TN 37923 | Preschool |
| La Petite Academy of Knoxville | 6915 Harrell Road, Knoxville, TN 37931 | Preschool |
| La Petite Academy of Knoxville | 712 E. Emory Road, Knoxville, TN 37938 | Preschool |
| Powell KinderCare | 3053 Staffordshire Blvd, Powell, TN 37849 | Preschool |
| A Bountiful Blessing Learning Center | 3915 Carnation, Knoxville, TN 37921 | Center |
| ABC Kiddie Academy | 3100 Linden Ave, Knoxville, TN 37914 | Center |
| Abundant Love Child Care | 2649 Wilson Avenue, Knoxville, TN 37914 | Center |
| Active Learning Child Development Center | 1226 Mercer Avenue, Knoxville, TN 37921 | Center |
| Alice In Wonderland I GDCH | 1506 Ohio Avenue, Knoxville, TN 37921 | Center |
| Alice In Wonderland II | 3327 Thomas Street, Knoxville, TN 37921 | Center |
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| All About Me Academy | 2525 Merchants Road, Knoxville, TN 37912 | Center |
| All My Children Child Care | 2628 Linden Avenue, Knoxville, TN 37914 | Center |
| Anderson - South Head Start | 4808 Prospect Road, Knoxville, TN 37920 | Preschool |
| Andujar's Room | 1750 Connecticut Avenue, Knoxville, TN 37921 | Center |
| Apostolic Kiddie Academy | 5020 Pleasant Ridge Road, Knoxville, TN 37912 | Preschool |
| Baba's Place | 1209 Pickwick Road, Knoxville, TN 37914 | Center |
| Back To Basics Child Care | 209 Deaderick Avenue, Knoxville, TN 37921 | Center |
| Barney's Day Care | 1701 Louisiana Avenue, Knoxville, TN 37921 | Center |
| Bearden United Methodist Church Childcare & Afterschool Care | 4407 Sutherland Ave, Knoxville, TN 37919 | Center |
| Beaumont Magnet School Pre-kindergarten | 1211 Beaumont Avenue, Knoxville, TN 37921 | Preschool |
| Beaver Dam Baptist Child Care | 4328 Emory Road, Knoxville, TN 37938 | Preschool |
| Belle Morris Elementary Pre-kindergarten | 2308 Washington Pike, Knoxville, TN 37917 | Preschool |
| Bonny-Kate Elementary Pre-kindergarten | 7608 Martin Mill Pike, Knoxville, TN 37920 | Preschool |
| Bountiful Blessings | 3835 Catalpa Avenue, Knoxville, TN 37914 | Center |
| Brickey-Mccloud Elementary Pre-kindergarten | 1810 Dry Gap Pike, Knoxville, TN 37918 | Preschool |
| Bundles Of Love | 1600 Pequoid Street, Knoxville, TN 37915 | Center |
| Cedar Bluff Playschool | 1030 Old Cedar Bluff Road, Knoxville, TN 37923 | Center |
| Cedar Bluff Preschool | 709 N. Cedar Bluff Road, Knoxville, TN 37923 | Preschool |
| Cedar Springs Preschool - Kindergarten | 9132 Kingston Pk., Knoxville, TN 37923 | Center |
| Cedar Springs Weekday School | 9132 Kingston Pk., Knoxville, TN 37923 | Preschool |
| Central Baptist Bearden Preschool | 6300 Deane Hill Drive, Knoxville, TN 37919 | Preschool |
| Central Baptist Bearden School Age Program | 6300 Deane Hill Drive, Knoxville, TN 37919 | Center |
| Central Baptist Fountain City Early Childcare | 5364 N Broadway, Knoxville, TN 37918 | Preschool |
| Central Baptist Fountain City School Age Program | 5364 N Broadway, Knoxville, TN 37918 | Center |
| Chapman Learning Center | 5101 Chapman Highway, Knoxville, TN 37920 | Center |
| Chapman Toddler Learning Center | 5101 Chapman Highway, Knoxville, TN 37920 | Center |
| Child & Family Tennessee - Cooper House | 1206 Luttrell Street, Knoxville, TN 37917 | Center |
| Child & Family Tennessee - Katie Miller II | 3006 Lakebrook Boulevard, Knoxville, TN 37909 | Center |
| Child & Family Tennessee Great Starts Nursery | 3006 Lake Brook Blvd., Knoxville, TN 37909 | Preschool |
| Child Creations | 102 W. Copeland Road, Powell, TN 37849 | Center |
| Children At Play Learning Center | 2440 Magnolia Avenue, Knoxville, TN 37917 | Center |
| Childrens Center Of Knoxville | 301 Frank Street, Knoxville, TN 37919 | Preschool |
| Christenberry Elementary Pre-kindergarten | 927 Oglewood Avenue, Knoxville, TN 37917 | Preschool |
| Christian's Preschool | 6808 Northshore Drive, Knoxville, TN 37919 | Preschool |
| Christus Victor Lutheran Early Child Daycare | 4110 Cental Ave. Pike, Knoxville, TN 37912 | Preschool |
| Church Street United Methodist Church Preschool | 900 Henley St., Knoxville, TN 37902 | Preschool |
| Claxton - West Head Start | 2400 Piedmont Street, Knoxville, TN 37921 | Preschool |
| Cozy Kids | 3508-f Maryville Pike, Knoxville, TN 37920 | Center |
| Crawford's Group Day Care Home | 4930 Lonas Drive, Knoxville, TN 37909 | Center |
| Davis Center For Child Development | 500 Hotel Rd, Knoxville, TN 37918 | Center |
| Dogwood Elementary Pre-kindergarten | 705 Tipton Avenue, Knoxville, TN 37920 | Preschool |
| Doves From Heaven | 4300 Mascarene Road, Knoxville, TN 37921 | Center |
| E L Fletcher Academy | 239 E Ast Oak Hill Avenue, Knoxville, TN 37917 | Center |
| East Knoxville Learning Center | 3022 Linden Ave, Knoxville, TN 37914 | Center |
| Ebenezer Children's Academy | 1532 Ebenezer Rd, Knoxville, TN 37922 | Center |
| Fair Garden Center title I Preschool | 400 Fern Street, Knoxville, TN 37914 | Preschool |
| Favorite Friends Day Care | 744 Kimberlin Heights Road, Knoxville, TN 37920 | Center |
| Fifth Ave Bapt Child Care Center | 2500 East Fifth Ave, Knoxville, TN 37914 | Center |
| First Lutheran Early Childhood Education | 1207 Broadway, Knoxville, TN 37917 | Center |
| First Lutheran Early Childhood Education | 1226 Armstrong, Knoxville, TN 37917 | Preschool |
| First Step II - Old Broadway | 4605 Old Broadway, Knoxville, TN 37918 | Center |
| First Step II - Young Adventures | 520 Sevier Ave, Knoxville, TN 37920 | Center |
| First United Methodist Child Development Center | 3316 Kingston Pike, Knoxville, TN 37919 | Preschool |
| Forever Increasing Academy | 1749 Connecticut Avenue, Knoxville, TN 37921 | Center |
| Fort Sanders Pre-kindergarten | 501 21st Street, Knoxville, TN 37916 | Preschool |
| Fountain City United Methodist Church Preschool | 212 Hotel Rd, Knoxville, TN 37918 | Preschool |
| Freedom Christian Academy Early Childcare | 4615 Ashville Highway, Knoxville, TN 37914 | Preschool |
| Future Generation Learning Center | 1310 Hollywood Drive, Knoxville, TN 37909 | Preschool |
| Garden Montessori School | 3225 Garden Drive, Knoxville, TN 37918 | Preschool |
| Garden Of Discovery Learning Center | 1601 Dandridge Avenue, Knoxville, TN 37915 | Preschool |
| Garden Of Eden Preschool | 2329 Prosser Road, Knoxville, TN 37914 | Preschool |
| Genesis Preschool And Learning Center | 2544 Wayland Ave, Knoxville, TN 37914 | Preschool |
| Gods Creative Enrichment Center | 2647 Woodbine Avenue, Knoxville, TN 37914 | Center |
| Grace Christian Preparatory Kindergarten | 7171 Oak Ridge Hwy., Knoxville, TN 37931 | Center |
| Grace Place After School | 7171 Oak Ridge Hwy., Knoxville, TN 37931 | Center |
| Green Magnet School Pre-kindergarten | 801 Townview Drive, Knoxville, TN 37915 | Preschool |
| Halls Elementary Idea 619 Program | 7502 Anderson Pike, Knoxville, TN 37938 | Preschool |
| Halls-Powell Boys & Girls Clubs | 1819 Dry Gap Road, Knoxville, TN 37918 | Center |
| Happy Family Day Care | 212 Grata Road, Knoxville, TN 37914 | Center |
| Haslam - Boys & Girls Club | 407 Caswell Avenue, Knoxville, TN 37917 | Center |
| Helen's Baby Care | 2114 Sycamore Dr., Knoxville, TN 37921 | Center |
| Helping Hands Child Care | 6900 Office Park Circle, Knoxville, TN 37909 | Center |
| Helping Hands Kids Korner | 6228 Washington Pike, Knoxville, TN 37918 | Center |
| Jenny's Heavenly Angels | 3232 Leland Street, Knoxville, TN 37917 | Center |
| Johnson University Early Child Learning Center | 7906 Eubanks Drive, Knoxville, TN 37998 | Center |
| Just For Kids | 1206 Trigg Street, Knoxville, TN 37915 | Center |
| Kids Are Special | 3110 Keith Avenue, Knoxville, TN 37921 | Center |
| Kids At Play | 404 Kendall Road, Knoxville, TN 37919 | Center |
| Kids First | 6700 Central Ave. Pike, Knoxville, TN 37912 | Center |
| Kids Place - Amherst | 5101 Schaad Road, Knoxville, TN 37931 | Center |
| Kids Place At Sequoyah | 942 Southgate Circle, Knoxville, TN 37919 | Center |
| Kids Place Chilhowee | 5005 Asheville Hwy, Knoxville, TN 37914 | Center |
| Kids Place Fountain City | 2910 Montbelle Drive, Knoxville, TN 37918 | Center |
| Kids Place Inskip | 4701 High School Road, Knoxville, TN 37912 | Center |
| Kids Place West View | 1714 Mingle Avenue, Knoxville, TN 37921 | Center |
| Kidtime - Dogwood Elementary | 705 Tipton Ave., Knoxville, TN 37920 | Center |
| Kidz Inkorporated Preschool | 2355 East Magnolia Ave, Knoxville, TN 37915 | Preschool |
| Kierra's Playhouse | 3404 Bishop Street, Knoxville, TN 37921 | Center |
| King's Castle Learning Center | 6140 Western Avenue, Knoxville, TN 37921 | Center |
| Kiwanis -East Head Start II | 2330 Prosser Road, Knoxville, TN 37914 | Preschool |