| La Petite Academy of Lakeland | 1940 Glendale Street, Lakeland, FL 33803 | Preschool |
| A Home Away From Home | 1129 1st St S, Winter Haven, FL 33880 | Center |
| A Home Away From Home II | 1169 1st Street S, Winter Haven, FL 33880 | Preschool |
| A'Kelynn Angels Learning Center | 800 Havendale Blvd. N.w., Winter Haven, FL 33881 | Center |
| All About Kidz Learning Center | 105 Avenue G Se, Winter Haven, FL 33880 | Center |
| All Saints Academy | 5001 State Road 540 West, Winter Haven, FL 33880 | Center |
| Alpha Kid Care at Highland City | 5335 9th Street Se, Highland City, FL 33846 | Center |
| Alpha Kids Learning Center | 1013 Lakeshore Dr, Lakeland, FL 33803 | Preschool |
| Berkley Charter School | 5240 Berkley Road, Auburndale, FL 33823 | Preschool |
| Bethel's Kids Kare | 123 Avenue Y Ne, Winter Haven, FL 33881 | Preschool |
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| Beymer Methodist Preschool | 700 North Lake Howard Dr, Winter Haven, FL 33881 | Center |
| Bright Futures Early Learning Enrichment Center | 5999 Dundee Road #150, Winter Haven, FL 33884 | Center |
| Building Blocks Academy | 229 Avenue K Se, Winter Haven, FL 33880 | Center |
| CT&S Annointed Learning Center II | 2302 Haywood Street, Lakeland, FL 33801 | Preschool |
| Calvary Child Care | 3800 Recker Highway, Winter Haven, FL 33880 | Center |
| Calvary Kids Preschool | 2101 Overlook Dr Se, Winter Haven, FL 33884 | Center |
| Chris' Child Care Center | 200 Avenue Q Ne, Winter Haven, FL 33881 | Preschool |
| Creative Minds Academy II | 1802 Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Creative Minds Learning Academy | 3108 Atlantic Ave, Lakeland, FL 33801 | Center |
| Creative World Learning Center | 1390 Magnolia St, Bartow, FL 33830 | Center |
| Cuyler Learning Center | 613 Bennett Street, Auburndale, FL 33823 | Center |
| Cuyler Learning Preschool and School Age | 170 Dairy Road, Auburndale, FL 33823 | Center |
| Cypress Oaks School | 3079 Cypress Gardens Rd, Winter Haven, FL 33884 | Preschool |
| Cypress Oaks School II | 2351 Register Rd, Winter Haven, FL 33884 | Preschool |
| Cypress Preschool and Learning Center | 124 Avenue G Se, Winter Haven, FL 33880 | Preschool |
| Discovery Learning Academy | 3915 Old Highway 37, Lakeland, FL 33813 | Preschool |
| Eagle Lake Christian Preschool | 670 N Eagle Drive, Eagle Lake, FL 33839 | Center |
| Eagles Nest Academy | 450 West Main Street, Bartow, FL 33830 | Preschool |
| Early Childhood Academy George Jenkins | 6000 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| Early Childhood Kidcare | 590 S. Wilson Avenue, Bartow, FL 33830 | Center |
| Early Childhood Kidcare | 4599 Cypress Gardens Rd, Winter Haven, FL 33884 | Center |
| Early Childhood Learning Center | 1703 Skinner Street, Lakeland, FL 33801 | Preschool |
| East Coast Migrant Headstart Project | 351 Frederick Avenue, Dundee, FL 33838 | Center |
| Ereen's Little Learners PreSchool | 595 Avenue L Se, Winter Haven, FL 33880 | Center |
| Fairyland Learning Center | 146 Avenue T Ne, Winter Haven, FL 33881 | Preschool |
| First Academy Preschool | 1400 Us Hwy 92 W, Winter Haven, FL 33881 | Preschool |
| First Academy Preschool Auburndale | 614 Main Street South, Auburndale, FL 33823 | Preschool |
| First Baptist Church of Highland City Preschool | 5410 Yarborough Lane, Lakeland, FL 33812 | Preschool |
| First Methodist School | 455 South Broadway Avenue, Bartow, FL 33830 | Center |
| First Missionary Baptist Child Care and Preschool | 200 Avenue R Nw, Winter Haven, FL 33881 | Center |
| Fruit of the Spirit Child Care Center II | 1615 5th Street Se, Winter Haven, FL 33880 | Center |
| Fruit of the Spirit Childcare Center | 549 Avenue T Ne, Winter Haven, FL 33881 | Center |
| Future Children Development Center | 2128 S Crystal Lake Dr, Lakeland, FL 33801 | Center |
| Futurecare Learning Center | 412 Old Winter Haven Road, Auburndale, FL 33823 | Preschool |
| Gifts 4 Life Christian Academy | 630 Waldron Avenue, Bartow, FL 33830 | Center |
| Gloryland Express II | 490 West Davidson Street, Bartow, FL 33830 | Preschool |
| Goal Seekers and Eagles Before and After School Program | 4730 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| God Little Helpers | 1641 E Memorial Blvd, Lakeland, FL 33801 | Center |
| God's Tots Learning Center | 875 E. Main Street, Bartow, FL 33830 | Center |
| Grace Lutheran Early Childhood | 330 Avenue C Se, Winter Haven, FL 33880 | Center |
| Hands-N-Hearts in Motion | 3330 Winterlake Road, Lakeland, FL 33803 | Preschool |
| Haven Christian Academy | 2105 King Rd Sw, Winter Haven, FL 33880 | Center |
| Heavenly Kids Christian Academy | 2605 Sunshine Drive South, Lakeland, FL 33801 | Center |
| Henderson Children of Glory Childcare Center | 109 N 3rd St, Eagle Lake, FL 33839 | Center |
| Heritage Christian Academy | 244 Avenue D Sw, Winter Haven, FL 33880 | Center |
| Highlands Christian Academy | 4210 Lakeland Highlands Rd., Lakeland, FL 33813 | Center |
| Highways To Tomorrow | 801 N Broadway Ave, Bartow, FL 33830 | Preschool |
| Hope Creative School | 2110 Cypress Gardens Blvd, Winter Haven, FL 33884 | Center |
| Imagination Station of Polk County | 1200 34th St Nw, Winter Haven, FL 33881 | Preschool |
| JC's Early Learning Center | 2540 S Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Jeanni's Preschool and School Age | 905 Spirit Lake Rd, Winter Haven, FL 33880 | Center |
| Kiddie Korner | 400 Avenue C Southeast, Winter Haven, FL 33880 | Preschool |
| Kids Town Preschool | 333 Griffin Avenue, Lakeland, FL 33801 | Preschool |
| Kids World Enrichment Center | 1860 East Gibbons St., Bartow, FL 33830 | Center |
| Kidz Life After School | 915 South Broadway, Bartow, FL 33830 | Center |
| Learning Tree Child Development Center | 2995 Avenue G Nw, Winter Haven, FL 33880 | Preschool |
| Lil Champs Learning Center | 1801 Havendale Blvd, Winter Haven, FL 33881 | Center |
| Lit'le Bloodhound Pre-school | 222 Ariana Avenue, Auburndale, FL 33823 | Preschool |
| Little Land Early Learning | 235 Main Street, Dundee, FL 33838 | Preschool |
| Little Leaders of Tomorrow Child Care Center | 850 Forrest Drive, Bartow, FL 33830 | Preschool |
| Little Shepherds Preschool | 175 Lake Hollingsworth Drive, Lakeland, FL 33801 | Center |
| McCloud's Childcare Center | 1741 E Gary Road, Lakeland, FL 33801 | Preschool |
| Medulla Baptist Church Preschool Learning Center | 3930 Old Hwy 37, Lakeland, FL 33813 | Center |
| Miss Adele's Learning Center | 1804 Richmond Road, Lakeland, FL 33803 | Preschool |
| Mrs Heather's Lit'l Learning Center | 217 Palmetto St, Auburndale, FL 33823 | Center |
| Multi Level Tutoring | 824 Havendale Blvd Nw, Winter Haven, FL 33881 | Preschool |
| New Beginnings Preschool Learning Center | 5965 Sr 542 West, Winter Haven, FL 33880 | Preschool |
| New Life Kingdom Kids Child Care | 2101 2nd St. Ne, Winter Haven, FL 33881 | Center |
| Parkland Baptist Child Care Center | 1800 8th Street Se, Winter Haven, FL 33880 | Center |
| Pierre's Kiddieland | 225 Avenue B Nw, Winter Haven, FL 33881 | Center |
| Precious Ones Learning Center | 234 Noxon Street, Auburndale, FL 33823 | Center |
| Preparing the Way Ministries Enrichment Center | 1712 West Chase Street, Lakeland, FL 33805 | Center |
| R.E.A.L. Kidz Enrichment Center | 2195 E Georgia St, Bartow, FL 33830 | Center |
| RCMA Eloise Child Development Center | 710 Snively Ave, Winter Haven, FL 33880 | Preschool |
| RCMA Wahneta Child Development Center | 1920 Gerber Dairy Road, Winter Haven, FL 33880 | Preschool |
| Reach For Success | 768 31st Court Nw, Winter Haven, FL 33881 | Center |
| Redeemer Lutheran Christian Preschool | 585 E Summerlin St, Bartow, FL 33830 | Center |
| Richardson's Learning Enrichment Center | 1426 W Bryant St, Bartow, FL 33830 | Preschool |
| Ridge Teen Parent | 7700 State Road 544, Winter Haven, FL 33881 | Center |
| SACCA Boswell | 2820 K-ville Avenue, Auburndale, FL 33823 | Center |
| SACCA Brigham Academy Kid Care | 601 Avenue C Se, Winter Haven, FL 33880 | Center |
| SACCA Chain of Lakes Campus Kid Care | 7001 St Road 653, Winter Haven, FL 33884 | Center |
| SACCA Jewett Kid Care | 2250 8th St Ne, Winter Haven, FL 33880 | Center |
| SACCA Lake Shipp Kid Care | 250 Camellia Dr, Winter Haven, FL 33880 | Center |
| Safe and Sound Child Care and Early Learning Center | 580 East Laurel Ave, Eagle Lake, FL 33839 | Preschool |
| Shelby Street Station | 107 Shelby Street, Auburndale, FL 33823 | Preschool |
| Shirlene's Shining Stars Learning Center Too | 920 S Lake Shore Way, Lake Alfred, FL 33850 | Preschool |
| Small Beginnings Learning Center | 123 Avenue K Se, Winter Haven, FL 33880 | Center |
| Small Wonders Preschool | 4777 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| Small World Pre-School | 233 Bennett Street, Auburndale, FL 33823 | Preschool |