| Prince of Peace Lutheran Preschool | 1515 South Semoran Boulevard, Orlando, FL 32807 | Preschool |
| RJB Christian Childcare Center | 6308 West Colonial Drive, Orlando, FL 32818 | Center |
| RJM Learn-To-Read Childcare | 6573 Hidden Beach Cir, Orlando, FL 32819 | Center |
| Radiant Life Academy | 8151 Clarcona Ocoee Road, Orlando, FL 32818 | Center |
| Raise A Standard Academy | 6225 Clarcona Ocoee Road, Orlando, FL 32810 | Preschool |
| Raising Angels Child Care | 2385 West Church Street, Orlando, FL 32805 | Center |
| Raising Angels Child Development Center | 1720 Rio Grande Avenue, Orlando, FL 32805 | Center |
| Redeemer Lutheran Learning Center | 3377 Aloma Avenue, Winter Park, FL 32792 | Preschool |
| Regency Christian Academy | 11513 S Orange Blossom Trail, Orlando, FL 32837 | Center |
| Resurrection Preschool | 1211 Winter Garden Vineland Road, Winter Garden, FL 34787 | Preschool |
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| Richmond Heights Elementary School Preschool | 2500 Bruton Blvd., Orlando, FL 32811 | Preschool |
| Ridgewood Park Elementary Preschool | 3401 Pioneer Road, Orlando, FL 32818 | Preschool |
| Riverside Elementary School Preschool | 3125 Pembrook Ave, Orlando, FL 32810 | Preschool |
| Rock Lake Elementary School Preschool | 408 N. Tampa Ave, Orlando, FL 32805 | Preschool |
| Rolling Hills Elementary School Preschool | 4903 Donovan St, Orlando, FL 32808 | Preschool |
| Rollins College Child Development Center | 1000 Holt Ave Box 2760, Winter Park, FL 32789 | Center |
| Rosemary's Learning Center | 1446 39th Street, Orlando, FL 32839 | Preschool |
| Rosemont Elementary School Preschool | 4650 Point Lookout Rd, Orlando, FL 32808 | Preschool |
| Royal Academy Preschool | 5516 South Texas Avenue, Orlando, FL 32839 | Preschool |
| Royal Children's Academy | 4340 N Hiawassee Road, Orlando, FL 32818 | Preschool |
| Royal Children's Academy | 4340 N. Hiawassee Rd, Orlando, FL 32818 | Center |
| Sadler Elementary School Preschool | 4000 W. Oakridge Rd, Orlando, FL 32809 | Preschool |
| Salvation Christian Academy | 7085 S. Orange Blossom Trail, Orlando, FL 32809 | Center |
| Sea Shells Child Care | 1021 Pine Hills Road, Orlando, FL 32808 | Center |
| Sharon Wright's Christian Child Care Academy | 1041 North Pine Hills Road, Orlando, FL 32808 | Center |
| Shekinah Christian Academy | 340 South State Road 434 Suite ..., Altamonte Springs, FL 32714 | Preschool |
| Shingle Creek Elementary School Preschool | 5620 Harcourt Ave, Orlando, FL 32839 | Preschool |
| Silver Star Learning Center | 5386 Silver Star Road, Orlando, FL 32808 | Preschool |
| Silver Star Learning Center II | 5398 Silver Star Road, Orlando, FL 32808 | Preschool |
| Simply Growing | 4743 Raleigh Street, Orlando, FL 32811 | Center |
| Small Blessings Child Care | 1647 Peel Avenue, Orlando, FL 32806 | Preschool |
| Small Wonders Children's Center | 2856 Curry Ford Road, Orlando, FL 32806 | Preschool |
| Sonbeams Learning Center of Asbury United | 220 W Horatio Ave, Maitland, FL 32751 | Preschool |
| Sophia Kid's Learning Center | 1537 South Central Ave, Apopka, FL 32703 | Preschool |
| Southwood Head Start | 6225 Brookgreen Avenue, Orlando, FL 32839 | Preschool |
| Spring Lake Elementary Vpk | 695 Orange Avenue, Altamonte Springs, FL 32714 | Preschool |
| St Luke's Child Development Center | 4851 S Apopka Vineland Rd, Orlando, FL 32819 | Preschool |
| St Mark Children Enrichment Center | 4055 Covington Street, Orlando, FL 32811 | Preschool |
| St Mark Children Enrichment Center # 2 | 4087 Covington Street, Orlando, FL 32811 | Center |
| St Mary Magdalen Child Center | 710 Spring Lake Road, Altamonte Springs, FL 32701 | Preschool |
| St Mary's Preschool | 6316 Matchett Rd, Orlando, FL 32809 | Preschool |
| St Michael's Preschool | 2499 N Westmoreland Dr, Orlando, FL 32804 | Center |
| St Vincent's Anglican Academy | 1515 Edgewater Dr, Orlando, FL 32804 | Preschool |
| St. Charles Borromeo Catholic School | 4005 Edgewater Drive, Orlando, FL 32804 | Preschool |
| St. Richard's Preschool | 1720 Howell Branch Road, Winter Park, FL 32789 | Center |
| Star Child Academy of Winter Garden | 1324 Winter Garden Vineland Road, Winter Garden, FL 34787 | Center |
| Step By Step Learning Academy | 2335 S. Goldenrod Road, Orlando, FL 32822 | Preschool |
| Storybook School | 209 West Oak Ridge Road, Orlando, FL 32809 | Preschool |
| Sugar and Spice Learning Center & Child Care | 2606 S Ferncreek Ave, Orlando, FL 32806 | Preschool |
| Sunset Childcare | 4540 South Orange Blossom Trail, Orlando, FL 32839 | Preschool |
| Super Kids Academy | 4974 Millenia Boulevard # 101, Orlando, FL 32839 | Preschool |
| Super Kids Academy II | 4976 Millenia Boulevard, Orlando, FL 32839 | Preschool |
| Super Kids Academy Orlando | 2618 E. Robinson Street, Orlando, FL 32803 | Center |
| Super Star Winners Academy of Learning | 2517 Curry Ford Road, Orlando, FL 32806 | Preschool |
| Supreme Child Care | 5745 Edgewater Drive, Orlando, FL 32810 | Preschool |
| Sweet Dreams Child Care | 7230 W Colonial Dr, Orlando, FL 32818 | Preschool |
| TLC Christian Academy | 1408 W Michigan Street, Orlando, FL 32805 | Preschool |
| Taft Head Start Center | 9504 South Orange Avenue, Orlando, FL 32824 | Preschool |
| Tangelo Park Elementary School Preschool | 5115 Anzio St, Orlando, FL 32819 | Preschool |
| The Children's Center | 6900 Pershing Avenue, Orlando, FL 32822 | Preschool |
| The Children's Place Learning Center | 2095 Dundee Dr, Winter Park, FL 32792 | Preschool |
| The Garden Early Care and Learning Center | 1 South Cottage Hill Road, Orlando, FL 32805 | Preschool |
| The King's Academy | 1302 Edgeway Drive, Winter Garden, FL 34787 | Center |
| The Learning Castle | 1530 South Primrose Drive, Orlando, FL 32806 | Preschool |
| The Learning Center of South Park | 7350 Futures Drive Unit # 6, Orlando, FL 32819 | Preschool |
| The Learning Place Academy | 1616 Conway Gardens Road, Orlando, FL 32806 | Preschool |
| The Learning Tree | 1021 New York Avenue North, Winter Park, FL 32789 | Center |
| The Light Academy | 4895 West Colonial Drive, Orlando, FL 32808 | Center |
| The Little Red Train Pre-School and Child Care | 647 W Indiana St, Orlando, FL 32805 | Preschool |
| The Play Palace | 2817 North Powers Drive, Orlando, FL 32808 | Center |
| The Potter's House Academy and Day Care Center | 7051 Pershing Ave, Orlando, FL 32822 | Preschool |
| The Source After School Program | 8624 A. D. Mims Road, Orlando, FL 32818 | Center |
| The Tree House Educational Center | 1851 S Goldenrod Rd, Orlando, FL 32822 | Preschool |
| Three Points Elementary School Preschool | 4001 S. Goldenrod Road, Orlando, FL 32822 | Preschool |
| Tiny Praying Hands Christian Academy | 1259 N Pine Hills Road, Orlando, FL 32808 | Center |
| Tiny Praying Hands II | 1438 N Pine Hills Road, Orlando, FL 32808 | Center |
| Tiny Praying Hands III | 1440 North Pine Hills Road, Orlando, FL 32808 | Center |
| Tiny Treasures Learning Center | 800 Paul Street, Orlando, FL 32808 | Preschool |
| Today's Kids Day Care Center | 2625 Hiawasse Rd, Orlando, FL 32818 | Preschool |
| Toddler Trails Learning center | 1012 North Pine Hills Road, Orlando, FL 32808 | Center |
| Tomorrow's Academy | 3121 Monte Carlo Trail, Orlando, FL 32805 | Center |
| Toni's Touch Pre-K | 1301 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Tots in Toyland Learning Center | 11235 West Michigan Street, Orlando, FL 32805 | Preschool |
| Trinity Child Development Center | 420 & 424 Ruth Ln, Orlando, FL 32801 | Preschool |
| Trinity Christian Academy | 2113 East South Street, Orlando, FL 32803 | Center |
| Trinity Christian Early Childhood | 1022 S Orange Blossom Trail, Apopka, FL 32703 | Preschool |
| True Deliverance Center Day Care | 29 South Ivey Lane, Orlando, FL 32811 | Center |
| Tutor Time Child Care | 2275 Whisper Lakes Boulevard, Orlando, FL 32837 | Preschool |
| Tutor Time Childcare Learning Center | 1025 South Clarke Road, Ocoee, FL 34761 | Preschool |
| Unique Child Care Center | 1373 Bunnell Rd, Apopka, FL 32703 | Preschool |
| United Cerebral Palsy - West Orange | 630 S Dillard Street, Winter Garden, FL 34787 | Preschool |
| United Cerebral Palsy - Pine Hills Child Development | 5800 Golf Club Parkway, Orlando, FL 32818 | Preschool |
| United Cerebral Palsy of Central FL - Hollow | 3305 S. Orange Avenue, Orlando, FL 32806 | Preschool |
| Universal Child Care Development | 1003 South Kirkman Road, Orlando, FL 32811 | Preschool |
| Ventura Elementary Preschool | 4400 Woodgate Blvd, Orlando, FL 32822 | Preschool |
| Victory Christian Academy | 1601 A.d. Mims Rd, Ocoee, FL 34761 | Center |
| Washington Shores Elementary School Preschool | 944 West Lake Mann Drive, Orlando, FL 32805 | Preschool |
| Way of Grace Academy & Learning Center | 8550 Clarcona Ocoee Road, Orlando, FL 32818 | Center |
| We Love Kids | 636 N Wymore Road, Winter Park, FL 32789 | Preschool |
| Wee Kare Child Care Learning Academy | 5020 Silver Star Road, Orlando, FL 32808 | Preschool |