| Little Giants Learning Academy | 10891 Hackney Drive, Riverview, FL 33578 | Center |
| Little Munchkins | 702 S. Ever Street, Plant City, FL 33563 | Center |
| Little Stars Learning Academy | 706 N. Parsons Avenue, Brandon, FL 33510 | Center |
| Mango Head Start EHS | 11210 E. Broadway Avenue, Seffner, FL 33584 | Preschool |
| Mary Go Round Child Care Center | 10810 Hanaway Drive, Riverview, FL 33578 | Preschool |
| Mis' Ginny's | 1502 N. Parsons Avenue, Brandon, FL 33510 | Center |
| Mis' Ginny's - Hwy 92 | 11825 E. Highway 92, Seffner, FL 33584 | Center |
| Mis' Ginny's Inc - Lakewood | 119 S. Lakewood Drive, Brandon, FL 33511 | Center |
| Morning Glory Preschool | 6001 Christy Lane, Riverview, FL 33578 | Preschool |
| Nativity Catholic School | 705 E. Brandon Boulevard, Brandon, FL 33511 | Center |
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| New Jerusalem Christian Academy | 3101 Kingsway Road, Seffner, FL 33584 | Preschool |
| Plant City Head Start Center | 1304 Alsobrook Street, Plant City, FL 33563 | Preschool |
| Plant City High Little School | 1 Raider Place, Plant City, FL 33563 | Preschool |
| Plant City Learning Center | 651 E. Alexander Street, Plant City, FL 33563 | Center |
| Post Sunshine Ranch | 1350 John Moore Road, Brandon, FL 33511 | Preschool |
| Precious Jewells Day Care & Nursery | 5016 Garden Lane, Tampa, FL 33610 | Center |
| Progress Village Academy | 8616 Progress Boulevard, Tampa, FL 33619 | Center |
| Providence Christian School Preschool | 5416 Providence Road, Riverview, FL 33569 | Center |
| RCMA Central Plant City Child Development Center | 508 Lakewood Avenue, Plant City, FL 33563 | Preschool |
| RCMA Dover Child Development Center | 3238 San Jose Mission Drive, Dover, FL 33527 | Preschool |
| RCMA Westside Village Child Development Center | 3102 Sammonds Road, Plant City, FL 33563 | Preschool |
| Resurrection Christian School Preschool | 3225 Bell Shoals Road, Brandon, FL 33511 | Center |
| Resurrection Early Childhood Center | 6819 Krycul Avenue, Riverview, FL 33578 | Center |
| Seffner Christian Academy | 11605 E. U S Highway 92, Seffner, FL 33584 | Center |
| Seminole Heights United Methodist Preschool | 509 E. Hanna Street, Brandon, FL 33510 | Preschool |
| Shiloh Baptist Church Preschool | 905 W. Terrace Street, Plant City, FL 33563 | Center |
| Shining Stars Preschool Academy | 603 E. Morgan Street, Brandon, FL 33510 | Preschool |
| Simmon's Achievement Center | 107 E. Clay Avenue, Brandon, FL 33510 | Preschool |
| Smart Kids Academy | 3512 W. Baker Street, Plant City, FL 33563 | Preschool |
| South Shore Learning Center | 11525 Big Bend Road, Riverview, FL 33569 | Preschool |
| St. Andrew's United Methodist Preschool | 3315 S. Bryan Road, Brandon, FL 33511 | Preschool |
| St. Clement Early Childhood Center | 1104 N. Alexander Street, Plant City, FL 33563 | Preschool |
| St. Stephens Catholic School | 10424 Saint Stephen Circle, Riverview, FL 33569 | Center |
| Staci's Learning Station | 10611 Cone Grove Road, Riverview, FL 33578 | Center |
| Strawberry Hills Head Start & Early Head Start | 701 Tillman Place, Plant City, FL 33566 | Preschool |
| Strawberry Patch | 2805 W. Reynolds Street, Plant City, FL 33563 | Center |
| Sunshine Academy | 7739 Gibsonton Drive, Gibsonton, FL 33534 | Preschool |
| Tambra's Tiny Steps Learning Academy II | 4922 N. 84th Street, Tampa, FL 33619 | Preschool |
| Temple Christian Academy | 2909 John Moore Road, Brandon, FL 33511 | Center |
| The Brandon Montessori School at River Hills | 4223 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| The Fun Factory of Palm River | 536 South Maydell Drive, Tampa, FL 33619 | Center |
| The Learning Train Academy | 736 W. Dr Mlk Blvd, Seffner, FL 33584 | Center |
| The Life Church "Kids Life" | 12001 Big Bend Road, Riverview, FL 33579 | Center |
| The Peacock's Plume Company | 1094 Bloomingdale Avenue, Valrico, FL 33596 | Center |
| The Riverview Montessori School at Rivercrest | 11520 Ramble Creek Drive, Riverview, FL 33569 | Preschool |
| The Village Early Learning Center | 3741 Canoga Park Drive, Brandon, FL 33511 | Preschool |
| The Word Of His Grace L.D.C. Preschool | 2506 S. Parsons Avenue, Seffner, FL 33584 | Preschool |
| Think Loved Children | 408 E. Lumsden Road, Brandon, FL 33511 | Center |
| Tots Active Learning Center | 121 W. Prosser Drive, Plant City, FL 33563 | Center |
| Tropical Acres Christian Academy | 12107 Rhodine Road, Riverview, FL 33569 | Center |
| Valrico Child Care | 1405 S. St. Cloud Avenue, Valrico, FL 33594 | Center |
| Walden Lake Early Learning Center | 2730 Turkey Creek Road, Plant City, FL 33566 | Preschool |
| Wee Care Learning Academy | 219 Skywood Dr, Valrico, FL 33594 | Center |
| Westminster Academy | 402 E. Lumsden Road, Brandon, FL 33511 | Preschool |
| Wright's Wonderland II - Wrights Headstart | 7815 Tidewater Trail, Tampa, FL 33619 | Preschool |
| Wright's Wonderland of Learning Child Care Center | 812 S. Maydell Drive, Tampa, FL 33619 | Center |
| Ybor Early Childhood Learning Center East | 6815 E. 21st Avenue, Tampa, FL 33619 | Center |
| Younger Years Learning Center | 1905 Lenna Avenue, Seffner, FL 33584 | Center |