| Bell Shoals KinderCare | 4304 Bell Shoals Rd, Valrico, FL 33596 | Preschool |
| La Petite Academy of Brandon | 808 W. Bloomingdale Avenue, Brandon, FL 33511 | Preschool |
| La Petite Academy of Brandon | 606 E. Windhorst Road, Brandon, FL 33510 | Preschool |
| La Petite Academy of Riverview | 10025 Cristina Drive, Riverview, FL 33569 | Preschool |
| La Petite Academy of Valrico | 1011 Greenhollow Lane, Valrico, FL 33596 | Center |
| Seffner KinderCare | 2524 S Parsons Ave, Seffner, FL 33584 | Preschool |
| Walden Lake KinderCare | 1701 W Timberlane Dr, Plant City, FL 33566 | Preschool |
| A + Learning Center | 221 W. Alexander Street, Plant City, FL 33563 | Preschool |
| A Bridge of Learning | 3902 Corporex Park Drive, Tampa, FL 33619 | Center |
| A Child's Haven Private Preschool | 1520 Brentwood Hills Boulevard, Valrico, FL 33594 | Preschool |
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| A Children's Kastle Early Learning Center | 950 S. Mulrennan Road, Valrico, FL 33594 | Preschool |
| A Little Kids Academy | 11105 Us Hwy 92 East, Seffner, FL 33584 | Preschool |
| A Plus Child Care Center | 3042 S. 78th Street, Tampa, FL 33619 | Center |
| Above and Beyond Childcare | 9472 Mcintosh Road, Dover, FL 33527 | Center |
| Academy of Achievers | 536 Maydell Drive, Tampa, FL 33619 | Preschool |
| All Nations Preschool | 10022 Cristina Drive, Riverview, FL 33569 | Preschool |
| Almand Center | 1311 Brooker Road, Brandon, FL 33511 | Preschool |
| Almost Angels Academy | 11702 Taylor Road, Thonotosassa, FL 33592 | Center |
| Apostles Lutheran Church Preschool | 200 N. Kingsway Road, Brandon, FL 33510 | Preschool |
| Autumn Leaf Academy | 3007 Daniels Road, Plant City, FL 33566 | Preschool |
| B & G Day Care and Kindergarten | 14419 Dr Mlk Boulevard, Dover, FL 33527 | Preschool |
| Bealsville Head Start Center | 5100 Horton Road, Plant City, FL 33567 | Preschool |
| Bell Shoals Baptist Academy | 2102 Bell Shoals Road, Brandon, FL 33511 | Center |
| Bloomingdale Academy | 11418 Bloomingdale Avenue, Riverview, FL 33578 | Center |
| Boys & Girls Club Dover | 2820 South Gallagher Road, Dover, FL 33527 | Center |
| Brandon Academy | 801 Limona Road, Brandon, FL 33510 | Preschool |
| Brandon Day School | 202 Pauls Drive, Brandon, FL 33511 | Center |
| Brandon Head Start | 9547 E. Columbus Drive, Tampa, FL 33619 | Preschool |
| Brandon Sports and Aquatic Center | 405 Beverly Blvd, Brandon, FL 33511 | Preschool |
| Bright Beginnings | 2103 Mud Lake Road, Plant City, FL 33566 | Center |
| Brighter Kids | 2207 Durant Road, Valrico, FL 33596 | Center |
| Brookwood Academy | 3820 Coconut Palm Drive, Tampa, FL 33619 | Center |
| Browns Town Daycare | 7739 GIBSONTON DR, Gibsonton, FL 33534 | Center |
| Castles of Imagination | 820 Bryan Road, Brandon, FL 33511 | Preschool |
| Central Baptist Christian School | 402 E. Windhorst Road, Brandon, FL 33510 | Center |
| Child-Kare Solutions | 11002 E. Broadway Avenue, Seffner, FL 33584 | Preschool |
| Childcare of Brandon | 730 Brandon Town Center Dr, Brandon, FL 33511 | Center |
| Children's Academy | 506 Limona Road, Brandon, FL 33510 | Preschool |
| Children's Life Academy | 3120 S. Miller Road, Valrico, FL 33596 | Center |
| Children's Nest Day School | 1311 Mendonsa Road, Plant City, FL 33563 | Preschool |
| Children's Nest Day School | 3221 S. Bryan Road, Brandon, FL 33511 | Preschool |
| Circle M Ranch Day Care | 1202 Bloomingdale Avenue, Valrico, FL 33596 | Preschool |
| Citigroup Family Center | 3701 Queen Palm Drive, Tampa, FL 33610 | Center |
| Colour Their World | 115-a Margaret Street, Brandon, FL 33511 | Preschool |
| Community Christian Child Care | 4215 Orient Road, Tampa, FL 33610 | Preschool |
| Country Kids | 3802 N. Gallagher Road, Plant City, FL 33565 | Center |
| Creative World Lil Pals | 1010 S. 76th Street, Tampa, FL 33619 | Preschool |
| Creative World School Fish Hawk | 5525 Osprey Ridge Drive, Lithia, FL 33547 | Preschool |
| Creative World School at Rivercrest | 11361 Symmes Road, Riverview, FL 33569 | Preschool |
| Crossroads Baptist Church | 10405 S. Highway 39, Lithia, FL 33547 | Center |
| Dimples Playland Child Care Center - 12th Ave | 7445 S. 12th Avenue, Tampa, FL 33619 | Center |
| Discovery Point #54 | 3472 Kings Commercial Ct, Valrico, FL 33594 | Preschool |
| Discovery Point #60 | 11719 Symmes Road, Riverview, FL 33569 | Preschool |
| Discovery Point #70 | 13011 Summerfield Crossing Boulevard, Riverview, FL 33579 | Preschool |
| Easter Seals Child Development at Teen Parent East | 1202 Grant Street, Plant City, FL 33563 | Center |
| Faith Baptist Preschool | 1118 North Parsons Avenue, Brandon, FL 33510 | Center |
| First Baptist Brandon Christian Academy | 216 N. Parsons Ave., Brandon, FL 33510 | Center |
| First Discoveries Academy | 1311 Brooker Road, Brandon, FL 33511 | Center |
| First Presbyterian Learning Center | 404 W. Reynolds Street, Plant City, FL 33563 | Center |
| First Presbyterian Preschool of Brandon | 121 Carver Street, Brandon, FL 33510 | Preschool |
| First UMChurch Preschool and Kindergarten | 120 N. Knights Avenue, Brandon, FL 33510 | Preschool |
| Fish Hawk Early Learning Center | 5632 Osprey Park Place, Lithia, FL 33547 | Preschool |
| Fish Hawk Early Learning Center II | 6001 Parkset Drive, Lithia, FL 33547 | Preschool |
| Foundation Christian Academy | 3955 Lithia Pinecrest Road, Valrico, FL 33596 | Preschool |
| Fun Factory Preschool | 410 S. Collins Street, Plant City, FL 33563 | Preschool |
| Golden Rule Child Care Centers | 104 Golden Rule Lane, Plant City, FL 33566 | Center |
| Gospel Tabernacle Learning Center | 801 Wilkins Avenue, Plant City, FL 33563 | Center |
| HCPS - Clair-mel Elementary Head Start Vpk | 1025 South 78th Street, Tampa, FL 33619 | Preschool |
| HCPS - Dover Elementary Headstart Vpk | 3035 Nelson Ave., Dover, FL 33527 | Preschool |
| HCPS - Trapnell Elementary Headstart Vpk | 1605 West Trapnell Rd, Plant City, FL 33567 | Preschool |
| HCPS - West Tampa Elementary Headstart Vpk | 2700 Cherry St., Tampa, FL 33619 | Preschool |
| Happy Day Preschool | 722 S. Lithia Pinecrest Road, Brandon, FL 33511 | Preschool |
| Harvest Time Christian School | 1511 S. Us 301, Tampa, FL 33619 | Center |
| Harvest Time Christian School | 1511 U S 301 South, Tampa, FL 33619 | Center |
| Heaven Sent Day Care Center | 3003 S. Kings Avenue, Brandon, FL 33511 | Center |
| Hillsborough Baptiat School | 6021 Williams Road, Seffner, FL 33584 | Center |
| Hillsborough Baptist School | 6021 Williams Road, Seffner, FL 33584 | Center |
| Home Away From Home Daycare Center #2 | 7415 Destin Drive, Tampa, FL 33619 | Center |
| Home Away From Home Learning Center | 8516 Ridein Road, Tampa, FL 33619 | Center |
| Horizon Early Learning Preschool | 1720 S. Saint Cloud Avenue, Valrico, FL 33594 | Preschool |
| Humpty Dumpty Academy | 1211 Wishing Well Way, Tampa, FL 33619 | Center |
| Immanuel Lutheran Pre-school | 2913 John Moore Road, Brandon, FL 33511 | Preschool |
| It's Kidz Time Of Riverview | 11329 Big Bend Road, Riverview, FL 33569 | Center |
| Jay Care Enrichment Academy | 4609 Horton Road, Plant City, FL 33567 | Center |
| Kid's Community College - Lake St. Charles | 10544 Lake St. Charles Blvd, Riverview, FL 33578 | Preschool |
| Kid's Community College FishHawk Preschool | 16641 Fishhawk Boulevard, Lithia, FL 33547 | Preschool |
| Kids Club Early Learning Center | 1108 Goldfinch Drive, Plant City, FL 33563 | Center |
| Kids R Kids #8 FL | 4321 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| Kids R Tops | 215 N. Kingsway Road, Brandon, FL 33510 | Center |
| Kimberly's Infant Care Center | 605 S. Drane Street, Plant City, FL 33563 | Preschool |
| Kinder Kountry | 12660 Sydney Road, Dover, FL 33527 | Center |
| Kings Avenue Christian School | 2602 S. Kings Avenue, Brandon, FL 33511 | Center |
| Krestview Academy | 11425 Balm Riverview Road, Riverview, FL 33569 | Preschool |
| Lamb of God's Open Arms Early Childhood Center | 10540 Browning Road, Lithia, FL 33547 | Preschool |
| Lambkins | 1017 Kingsway Rd Rd Brandon, Brandon, FL 33510 | Center |
| Learn & Grow Pre-school | 2501 Durant Road, Valrico, FL 33596 | Preschool |
| Limona Village Chapel Children's Center | 408 Limona Road, Brandon, FL 33510 | Preschool |
| Linda's Little People | 1406 N. Wheeler Street, Plant City, FL 33563 | Center |
| Little Explorer's Learning Academy | 301 E. Alsobrook Street, Plant City, FL 33563 | Center |
| Little Footsteps Academy of Bloomingdale | 3721 Canoga Park Drive, Brandon, FL 33511 | Preschool |