| The Active Learner's Pre-School | 5719 W. Highway 22, Crestwood, KY 40014 | Preschool |
| The Chance School | 4200 Lime Kiln Lane, Louisville, KY 40222 | Preschool |
| The Chatter Box | 6317 Hwy 329, Prospect, KY 40059 | Preschool |
| The Children Center at Yum! Brands | 1900 Colonel Sanders Lane, Louisville, KY 40213 | Preschool |
| The Community Preschool of St. Matthew's Episcopal Church | 330 N. Hubbards Lane, Louisville, KY 40207 | Preschool |
| The Enrichment Center | 702 East Gray Street, Louisville, KY 40202 | Preschool |
| The Gardner School | 9401 Mill Brook Drive, Louisville, KY 40223 | Preschool |
| The Lively Children Childcare Center | 1140 Cherokee Road, Louisville, KY 40204 | Preschool |
| The Loving Place | 4200 Bardstown Road, Louisville, KY 40218 | Preschool |
| The Oak School | 138 North Evergreen Road, Louisville, KY 40243 | Preschool |
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| The Potter's House Childcare Enrichment Center | 314 South Wenzel Street, Louisville, KY 40204 | Preschool |
| The Vanguard Academy | 9306 Dayflower Street, Prospect, KY 40059 | Preschool |
| The Village Child Care Center | 5313 Hames Trace Court, Louisville, KY 40291 | Preschool |
| Tiny Hands Academy | 6718 Six Mile Lane, Louisville, KY 40218 | Preschool |
| Tiny Tot Nursery, Pre-School & Pre-Kindergarten | 908 Ormsby Lane, Louisville, KY 40242 | Preschool |
| Tomorrow's Promise Child Care and Learning Center | 6003 Pleasant Colony Court Suite 14, Crestwood, KY 40014 | Preschool |
| Trinity Presbyterian Preschool | 10200 Shelbyville Road, Louisville, KY 40223 | Preschool |
| Tully Elementary Childcare Enrichment Program | 3300 College Drive, Louisville, KY 40299 | Preschool |
| Visually Impaired Preschool Services | 1906 Goldsmith Lane, Louisville, KY 40218 | Preschool |
| Vivid Imaginations Child Care Center | 5925 Six Mile Lane, Louisville, KY 40218 | Preschool |
| Vivid Imaginations Child Care Center II | 3809 Bardstown Road, Louisville, KY 40218 | Preschool |
| Waldorf School of Louisville | 8005 New Lagrange Road, Louisville, KY 40222 | Preschool |
| Watch Me Grow In Excellence | 9115 Galene Drive, Louisville, KY 40299 | Preschool |
| Watkins Memorial United Methodist Preschool & Before and After Child Care & Mother's Day Out | 9800 Westport Road, Louisville, KY 40241 | Preschool |
| Watterson Elementary Childcare Enrichment Program | 3900 Breckinridge Lane, Louisville, KY 40218 | Center |
| Wee Care Nursery I, II & III | 11616 Main Street, Louisville, KY 40243 | Preschool |
| Westmore Academy & Preschool | 13306 West U.S. Highway 42, Prospect, KY 40059 | Preschool |
| Westport Road Baptist Church Child Care Center | 9705 Westport Road, Louisville, KY 40241 | Preschool |
| Westport Road Baptist Church Mother's Day Out - Preschool | 9705 Westport Road, Louisville, KY 40241 | Preschool |
| Westport Road Bible Preschool | 4500 Westport Road, Louisville, KY 40207 | Preschool |
| Wheeler Elementary Childcare Enrichment Program | 5700 Cynthia Drive, Louisville, KY 40291 | Preschool |
| Whitefield Academy Preschool East Campus | 15201 Shelbyville Road, Louisville, KY 40245 | Preschool |
| Whitehall Day Care Center | 2633 Whitehall Terrace, Louisville, KY 40220 | Preschool |
| Whiz Kidz Academy | 10634 Watterson Trail, Louisville, KY 40299 | Preschool |
| Wilder Elementary Childcare Enrichment Program | 1913 Herr Lane, Louisville, KY 40222 | Preschool |
| YMCA Christian Academy of Louisville - Rock Creek SACC Program | 3110 Rock Creek Road, Louisville, KY 40207 | Center |
| YMCA St. Leonard Afterschool Program | 440 Zorn Avenue, Louisville, KY 40206 | Preschool |
| YMCA St. Margaret Mary Afterschool Program | 7813 Shelbyville Road, Louisville, KY 40222 | Center |
| YMCA St. Patrick Afterschool Program | 1000 N. Beckley Station Road, Louisville, KY 40245 | Center |
| Zachary Taylor Elementary Childcare Enrichment Program | 9620 Westport Road, Louisville, KY 40241 | Preschool |