| Selwyn ASEP | 2840 Colony Road, Charlotte, NC 28211 | Center |
| Selwyn Presbyterian Child Development Center | 2929 Selwyn Avenue, Charlotte, NC 28209 | Center |
| Shamrock Gardens After School Enrichment Program | 3301 Country Club Drive, Charlotte, NC 28205 | Center |
| Sharon Elementary ASEP | 4330 Foxcroft Road, Charlotte, NC 28211 | Center |
| Sharon Montessori Academy | 1119 North Sharon Amity, Charlotte, NC 28211 | Preschool |
| Shelia's Wonderland | 1032 White Plains Rd, Charlotte, NC 28213 | Center |
| Shining Stars Academy - Ballantyne | 14115 Lancaster Highway, Charlotte, NC 28277 | Preschool |
| Shining Stars Academy Child Development Center | 910 East Arrowood Road, Charlotte, NC 28217 | Preschool |
| Shirley's Christian Day Care Home | 2711 Meadowcliff Dr, Charlotte, NC 28215 | Center |
| Small Treasures | 6318 Holly Knoll Drive, Charlotte, NC 28227 | Center |
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| Smart Kid Child Care And Education Center #1 | 225 Lambeth Drive, Charlotte, NC 28213 | Preschool |
| Smart Kids Child Development Center #3 | 6215 E Wt Harris Blvd, Charlotte, NC 28215 | Preschool |
| Smart Start Early Childhood Learning & Development Center | 4037 Sofley Road, Charlotte, NC 28206 | Center |
| Smarty Pants Full Day Preschool | 1411 East 7th Street, Charlotte, NC 28204 | Preschool |
| Smithfield Church Road Elementary ASEP | 3200 Smithfield Church Road, Charlotte, NC 28210 | Center |
| South Charlotte Baptist Church And Academy | 12416 Lancaster Highway, Pineville, NC 28134 | Preschool |
| St. John's Head Start | 300 Hawthorne Ln, Charlotte, NC 28204 | Preschool |
| St. Marks Child Development Center | 917 Clanton Road, Charlotte, NC 28217 | Center |
| St. Martin's Migrant Head Start NC - 41 | 15375 Spivey Corner Hwy, Charlotte, NC 28234 | Preschool |
| Stallings Elementary After School Program | 3501 Stallings Road, Matthews, NC 28104 | Center |
| Starfaith Child Care | 221 Short Hills Dr, Charlotte, NC 28217 | Center |
| Steele Creek ASEP | 4100 Gallont Ln, Charlotte, NC 28217 | Center |
| Step By Step Home Day Care | 4214 Sudbury Rd, Charlotte, NC 28205 | Center |
| Stephanie Jennings Education Center | 3320 North Sharon Amity Road, Charlotte, NC 28205 | Center |
| Stephanie's Sheltering Arms Daycare | 7724 Pence Pond Lane, Charlotte, NC 28227 | Center |
| Stepping Stone Home Day Care Center | 7038 Stillmeadow Drive, Charlotte, NC 28277 | Center |
| Sterling Elementary Afterschool Enrichment Program | 9701 China Grove Church Rd, Pineville, NC 28134 | Center |
| Stratford Richardson YMCA | 1946 West Blvd, Charlotte, NC 28208 | Center |
| Sugar Babies | 7637 Pence Pond Ln, Charlotte, NC 28227 | Center |
| Sunny Days After School | 12825 Hashanli Place, Matthews, NC 28105 | Center |
| Sunsetwood Academy | 1304 Eastway Drive, Charlotte, NC 28205 | Preschool |
| Sunshine Daycare And Preschool | 900 September Lane, Charlotte, NC 28208 | Preschool |
| Sunshine Daycare And Preschool | 3801 Glenwood Drive, Charlotte, NC 28208 | Preschool |
| Sunshine House Early Learning Academy - Baybrook Lane | 9239 Baybrook Lane, Charlotte, NC 28277 | Preschool |
| Sunshine House Early Learning Academy - Matthews | 1208 Sam Newell Road, Matthews, NC 28105 | Preschool |
| Superlative Academy | 1238 East 10th Street, Charlotte, NC 28204 | Center |
| Taylor's Home Day Care | 6214 Mayridge Dr, Charlotte, NC 28215 | Center |
| Teachers' Pet | 406 Silversmith Ln, Charlotte, NC 28270 | Preschool |
| Tegge's Learning Christian Enrichment Center | 2214 N Sharon Amity Road, Charlotte, NC 28205 | Center |
| Tele - Time Academy | 3426 Central Ave, Charlotte, NC 28205 | Center |
| Tender Hearts Child Care | 2301 Sharon Amity Rd N, Charlotte, NC 28205 | Center |
| The Charlotte Preparatory School | 212 Boyce Road, Charlotte, NC 28211 | Preschool |
| The Early Learning Center | 4304 S Tryon, Charlotte, NC 28217 | Center |
| The Learning Collaborative | 3045 North Davidison Street, Charlotte, NC 28205 | Preschool |
| The Learning Experience | 3937 West Arrowood Road, Charlotte, NC 28273 | Preschool |
| The Learning Experience | 11625 Providence Road West, Charlotte, NC 28277 | Preschool |
| The Learning Tree Enrichment Academy | 5500 Starkwood Drive, Charlotte, NC 28212 | Preschool |
| The Little Daisy's Child Care Center | 2008 Washington Ave, Charlotte, NC 28216 | Center |
| The Nest At Charlotte Latin School | 9502 Providence Road, Charlotte, NC 28277 | Preschool |
| The Sunshine House | 7532 Little Avenue, Charlotte, NC 28226 | Preschool |
| The Sunshine House - Ballantyne | 15644 Donnington Drive, Charlotte, NC 28277 | Preschool |
| The Sunshine House - Mint Hill | 5825 Phyliss Lane, Charlotte, NC 28227 | Center |
| The Village At Rainbow Station | 9829 Providence Road West, Charlotte, NC 28277 | Preschool |
| Thompson Child Development Center | 1645 Clanton Road, Charlotte, NC 28208 | Preschool |
| Tiaes And Joshua's Christian Playschool | 9827 Veronica Dr, Charlotte, NC 28215 | Center |
| Tiny Tots 2 Childcare | 2100 Eastway Drive, Charlotte, NC 28205 | Center |
| Today's Future Christian Academy | 2426 Holton Avenue, Charlotte, NC 28208 | Center |
| Toddlers University | 7231 Fox Hunt Road, Charlotte, NC 28212 | Center |
| Toddletown Kidz | 7328 Mossborough Ct, Charlotte, NC 28227 | Center |
| Tots Round The Clock | 3639 Eastway Dr, Charlotte, NC 28205 | Center |
| Tots World | 4432 Gaynelle Dr, Charlotte, NC 28215 | Center |
| University Park Elementary ASEP | 2400 Hildebrand Street, Charlotte, NC 28216 | Center |
| Victory Christian Center Preschool Building #1 | 7000 Old Pineville Road, Charlotte, NC 28217 | Preschool |
| Vision Love Day Care Home | 5424 Dennis Ct, Charlotte, NC 28213 | Center |
| Walter G. Byers Elementary ASEP | 1415 Hamilton Street, Charlotte, NC 28206 | Center |
| Westerly Hills Elementary ASEP | 4420 Denver Avenue, Charlotte, NC 28208 | Center |
| Westminster Presbyterian - SUNDAY SCHOOL ONLY | 101 Collville Road, Charlotte, NC 28207 | Center |
| Winterfield Elementary ASEP | 3100 Winterfield Place, Charlotte, NC 28205 | Center |
| Woodlawn Baptist Child Development Center | 4622 Nations Crossing Road, Charlotte, NC 28217 | Preschool |
| Word And Power Christian Academy | 501 N Linwood Ave, Charlotte, NC 28216 | Center |