| Oak Ridge Children's Academy & Day Care | 1118 & 1124 W Oakridge Road, Orlando, FL 32809 | Preschool |
| Oak Ridge YMCA Head Start Early Childhood | 814 West Oak Ridge Road, Orlando, FL 32809 | Center |
| Oakshire Elementary Preschool | 14501 Oakshire Blvd, Orlando, FL 32824 | Preschool |
| Ocoee Elementary School Preschool | 400 S. Lakewood Ave, Ocoee, FL 34761 | Preschool |
| Ocoee Oaks Preschool | 201 South Clarke Road, Ocoee, FL 34761 | Preschool |
| On The Rock Preschool | 801 Fairlane Ave., Orlando, FL 32809 | Center |
| Orange Center Elementary School Preschool | 631 Texas Ave, Orlando, FL 32839 | Preschool |
| Orlando Day Nursery | 626 Lake Dot Circle, Orlando, FL 32801 | Preschool |
| Orlando Early Learning Academy | 6261 Old Winter Garden Road, Orlando, FL 32835 | Preschool |
| Orlando Junior Academy | 30 East Evans Street, Orlando, FL 32804 | Preschool |
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| Orlando School of Cultural Dance | 412 N Pine Hills Road, Orlando, FL 32811 | Center |
| Orlando Science Center | 777 East Princeton Street, Orlando, FL 32803 | Preschool |
| Orlo Vista Elementary School Preschool | 3 N. Hastings St, Orlando, FL 32835 | Preschool |
| Palmetto Elementary School Preschool | 2015 Duskin Ave, Orlando, FL 32839 | Preschool |
| Park Lake Presbyterian Church Child Care Center | 309 East Colonial Drive, Orlando, FL 32801 | Preschool |
| Pastora Beula Child Care | 13 East Cypress Street, Winter Garden, FL 34787 | Center |
| Pathways School | 1877 West Oak Ridge Road, Orlando, FL 32809 | Center |
| Pathways School | 1877 W. Oakridge Rd, Orlando, FL 32809 | Preschool |
| Peabo Child Care Center | 815 Hills Street, Orlando, FL 32805 | Center |
| Peace United Methodist Preschool | 13502 Town Loop Blvd, Orlando, FL 32837 | Center |
| Peek A Boo Academy | 1222 North Pine Hills Road, Orlando, FL 32808 | Center |
| Peek-A-Boo Learning Center | 1304 N Pine Hill Rd, Orlando, FL 32808 | Preschool |
| Peekaboo Play and Learn | 825 Greenway Professional Court, Orlando, FL 32824 | Preschool |
| Peter Pan Child Care Center | 1602 Bruton Boulevard, Orlando, FL 32805 | Center |
| Piaget Academy | 6395 Raleigh Street, Orlando, FL 32835 | Preschool |
| Pine Hills Christian Child Care | 800 Pine Hills Road, Orlando, FL 32808 | Preschool |
| Pine Hills Elementary School Preschool | 1006 Ferndell Road, Orlando, FL 32808 | Preschool |
| Pine Hills Head Start Center | 6408 Jennings Road, Orlando, FL 32808 | Preschool |
| Pine Hills Preschool and ChildCare Center | 627 Pine Hills Road, Orlando, FL 32808 | Preschool |
| Pineloch Elementary School Preschool | 3101 Woods Ave, Orlando, FL 32805 | Preschool |
| Pinewood Elementary School Preschool | 3005 N Apopka Vineland Road, Orlando, FL 32818 | Preschool |
| Planet Learning | 1333 Florida Mall Avenue, Orlando, FL 32809 | Preschool |
| Power CARE Preschool Childcare and MDO Mother's Day Out | 3311 North Powers Drive, Orlando, FL 32818 | Preschool |
| Precious Creations Preschool | 201 North Kirkman Road, Orlando, FL 32811 | Center |
| Precious Gems Preschool | 2101 South Apopka Vineland Road, Orlando, FL 32835 | Preschool |
| Precious Hearts Daycare & Learning Center | 1320 44th Street, Orlando, FL 32809 | Preschool |
| Precious Times Learning Center | 630 Emeralda Rd Suite 104, Orlando, FL 32808 | Preschool |
| Premier Academy | 9380 Gotha Road, Windermere, FL 34786 | Center |
| Primary Prep Academy | 445 Gaston Foster Rd, Orlando, FL 32807 | Preschool |
| Primrose School of Hunters Creek | 5741 Town Center Boulevard, Orlando, FL 32837 | Preschool |
| RJB Christian Childcare Center | 6308 West Colonial Drive, Orlando, FL 32818 | Center |
| RJM Learn-To-Read Childcare | 6573 Hidden Beach Cir, Orlando, FL 32819 | Center |
| Raising Angels Child Care | 2385 West Church Street, Orlando, FL 32805 | Center |
| Raising Angels Child Development Center | 1720 Rio Grande Avenue, Orlando, FL 32805 | Center |
| Regency Christian Academy | 11513 S Orange Blossom Trail, Orlando, FL 32837 | Center |
| Resurrection Preschool | 1211 Winter Garden Vineland Road, Winter Garden, FL 34787 | Preschool |
| Richmond Heights Elementary School Preschool | 2500 Bruton Blvd., Orlando, FL 32811 | Preschool |
| Ridgewood Park Elementary Preschool | 3401 Pioneer Road, Orlando, FL 32818 | Preschool |
| Rock Lake Elementary School Preschool | 408 N. Tampa Ave, Orlando, FL 32805 | Preschool |
| Rolling Hills Elementary School Preschool | 4903 Donovan St, Orlando, FL 32808 | Preschool |
| Rosemary's Learning Center | 1446 39th Street, Orlando, FL 32839 | Preschool |
| Rosemont Elementary School Preschool | 4650 Point Lookout Rd, Orlando, FL 32808 | Preschool |
| Royal Academy Preschool | 5516 South Texas Avenue, Orlando, FL 32839 | Preschool |
| Royal Children's Academy | 4340 N Hiawassee Road, Orlando, FL 32818 | Preschool |
| Royal Children's Academy | 4340 N. Hiawassee Rd, Orlando, FL 32818 | Center |
| Sadler Elementary School Preschool | 4000 W. Oakridge Rd, Orlando, FL 32809 | Preschool |
| Salvation Christian Academy | 7085 S. Orange Blossom Trail, Orlando, FL 32809 | Center |
| Sea Shells Child Care | 1021 Pine Hills Road, Orlando, FL 32808 | Center |
| Sharon Wright's Christian Child Care Academy | 1041 North Pine Hills Road, Orlando, FL 32808 | Center |
| Shingle Creek Elementary School Preschool | 5620 Harcourt Ave, Orlando, FL 32839 | Preschool |
| Silver Star Learning Center | 5386 Silver Star Road, Orlando, FL 32808 | Preschool |
| Silver Star Learning Center II | 5398 Silver Star Road, Orlando, FL 32808 | Preschool |
| Simply Growing | 4743 Raleigh Street, Orlando, FL 32811 | Center |
| Small Blessings Child Care | 1647 Peel Avenue, Orlando, FL 32806 | Preschool |
| Small Wonders Children's Center | 2856 Curry Ford Road, Orlando, FL 32806 | Preschool |
| South Chase KinderCare - CLOSED | 12307 South Orange Blossom Trail, Orlando, FL 32837 | Preschool |
| Southwood Head Start | 6225 Brookgreen Avenue, Orlando, FL 32839 | Preschool |
| St Luke's Child Development Center | 4851 S Apopka Vineland Rd, Orlando, FL 32819 | Preschool |
| St Mark Children Enrichment Center | 4055 Covington Street, Orlando, FL 32811 | Preschool |
| St Mark Children Enrichment Center # 2 | 4087 Covington Street, Orlando, FL 32811 | Center |
| St Mary's Preschool | 6316 Matchett Rd, Orlando, FL 32809 | Preschool |
| St Michael's Preschool | 2499 N Westmoreland Dr, Orlando, FL 32804 | Center |
| St Vincent's Anglican Academy | 1515 Edgewater Dr, Orlando, FL 32804 | Preschool |
| St. Charles Borromeo Catholic School | 4005 Edgewater Drive, Orlando, FL 32804 | Preschool |
| Star Child Academy of Winter Garden | 1324 Winter Garden Vineland Road, Winter Garden, FL 34787 | Center |
| StarChild Academy - Crystal Creek | 12050 Crystal Commerce Way, Orlando, FL 32837 | Center |
| Storybook School | 209 West Oak Ridge Road, Orlando, FL 32809 | Preschool |
| Sugar and Spice Learning Center & Child Care | 2606 S Ferncreek Ave, Orlando, FL 32806 | Preschool |
| Sunchild Academy | 13740 Town Loop Boulevard, Orlando, FL 32837 | Preschool |
| Sunset Childcare | 4540 South Orange Blossom Trail, Orlando, FL 32839 | Preschool |
| Super Kids Academy | 4974 Millenia Boulevard # 101, Orlando, FL 32839 | Preschool |
| Super Kids Academy II | 4976 Millenia Boulevard, Orlando, FL 32839 | Preschool |
| Super Kids Academy Orlando | 2618 E. Robinson Street, Orlando, FL 32803 | Center |
| Super Star Winners Academy of Learning | 2517 Curry Ford Road, Orlando, FL 32806 | Preschool |
| Sweet Dreams Child Care | 7230 W Colonial Dr, Orlando, FL 32818 | Preschool |
| TLC Christian Academy | 1408 W Michigan Street, Orlando, FL 32805 | Preschool |
| Taft Head Start Center | 9504 South Orange Avenue, Orlando, FL 32824 | Preschool |
| Tangelo Park Elementary School Preschool | 5115 Anzio St, Orlando, FL 32819 | Preschool |
| The Garden Early Care and Learning Center | 1 South Cottage Hill Road, Orlando, FL 32805 | Preschool |
| The King's Academy | 1302 Edgeway Drive, Winter Garden, FL 34787 | Center |
| The Learning Castle | 1530 South Primrose Drive, Orlando, FL 32806 | Preschool |
| The Learning Center of South Park | 7350 Futures Drive Unit # 6, Orlando, FL 32819 | Preschool |
| The Learning Place Academy | 1616 Conway Gardens Road, Orlando, FL 32806 | Preschool |
| The Light Academy | 4895 West Colonial Drive, Orlando, FL 32808 | Center |
| The Little Red Train Pre-School and Child Care | 647 W Indiana St, Orlando, FL 32805 | Preschool |
| The Play Palace | 2817 North Powers Drive, Orlando, FL 32808 | Center |
| The Source After School Program | 8624 A. D. Mims Road, Orlando, FL 32818 | Center |
| Tiny Praying Hands Christian Academy | 1259 N Pine Hills Road, Orlando, FL 32808 | Center |
| Tiny Praying Hands II | 1438 N Pine Hills Road, Orlando, FL 32808 | Center |
| Tiny Praying Hands III | 1440 North Pine Hills Road, Orlando, FL 32808 | Center |