| Tiny Treasures Learning Center | 800 Paul Street, Orlando, FL 32808 | Preschool |
| Today's Kids Day Care Center | 2625 Hiawasse Rd, Orlando, FL 32818 | Preschool |
| Toddler Trails Learning center | 1012 North Pine Hills Road, Orlando, FL 32808 | Center |
| Tomorrow's Academy | 3121 Monte Carlo Trail, Orlando, FL 32805 | Center |
| Toni's Touch Pre-K | 1301 North Pine Hills Road, Orlando, FL 32808 | Preschool |
| Tots in Toyland Learning Center | 11235 West Michigan Street, Orlando, FL 32805 | Preschool |
| Treasure of Knowledge Christian Academy | 13001 Landstar Blvd., Orlando, FL 32824 | Center |
| Trinity Child Development Center | 420 & 424 Ruth Ln, Orlando, FL 32801 | Preschool |
| Trinity Christian Academy | 2113 East South Street, Orlando, FL 32803 | Center |
| True Deliverance Center Day Care | 29 South Ivey Lane, Orlando, FL 32811 | Center |
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| Tutor Time Child Care | 2275 Whisper Lakes Boulevard, Orlando, FL 32837 | Preschool |
| Tutor Time Childcare Learning Center | 1025 South Clarke Road, Ocoee, FL 34761 | Preschool |
| United Cerebral Palsy - West Orange | 630 S Dillard Street, Winter Garden, FL 34787 | Preschool |
| United Cerebral Palsy - Pine Hills Child Development | 5800 Golf Club Parkway, Orlando, FL 32818 | Preschool |
| United Cerebral Palsy of Central FL - Hollow | 3305 S. Orange Avenue, Orlando, FL 32806 | Preschool |
| Universal Child Care Development | 1003 South Kirkman Road, Orlando, FL 32811 | Preschool |
| Victory Christian Academy | 1601 A.d. Mims Rd, Ocoee, FL 34761 | Center |
| Washington Shores Elementary School Preschool | 944 West Lake Mann Drive, Orlando, FL 32805 | Preschool |
| Wee Kare Child Care Learning Academy | 5020 Silver Star Road, Orlando, FL 32808 | Preschool |
| Weekday School at First Presbyterian Church | 106 E. Church Street, Orlando, FL 32801 | Center |
| Wesley Child Development Center | 42 E. Jackson Street, Orlando, FL 32801 | Preschool |
| West Oaks Elementary School Preschool | 905 Dorscher Rd, Orlando, FL 32818 | Preschool |
| West Orlando Baptist Child Discovery Center | 1006 E Crown Point Rd, Ocoee, FL 34761 | Center |
| Westgate Children's Learning & Development Center | 7450 Sandlake Commons Boulevard, Orlando, FL 32819 | Preschool |
| Windermere Community Preschool | 8464 Winter Garden Vineland Rd, Orlando, FL 32836 | Center |
| Windermere Elementary Preschool | 11125 Park Avenue, Windermere, FL 34786 | Preschool |
| Windermere Union Church Preschool | 10710 Park Ridge Gotha Road, Windermere, FL 34786 | Preschool |
| Winegard Elementary School Preschool | 7055 Winegard Road, Orlando, FL 32809 | Preschool |
| Winners Preschool and Child Care Center III | 609 North Powers Drive Suite 308, Orlando, FL 32818 | Preschool |
| YMCA Family Center at Disney - Buena Vista | 2800 East Vista Boulevard, Orlando, FL 32830 | Preschool |
| Young Achievers | 11 East Lancaster Road, Orlando, FL 32809 | Preschool |