| D L C Nurse & Learn | 4101 College Street, Jacksonville, FL 32205 | Preschool |
| Dawn's Daycare and Learning Center | 1775 Cassat Ave, Jacksonville, FL 32210 | Preschool |
| Daystar Daycare & Learning Academy | 8061 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Deermeadows Preschool | 9780 Baymeadows Road, Jacksonville, FL 32256 | Preschool |
| Different Faces | 6221 Merrill Road, Jacksonville, FL 32277 | Center |
| Dream Kids Preschool & Arts Learning Center | 3980 Southside Blvd Ste#102, Jacksonville, FL 32257 | Preschool |
| ECS Early Learning at Exchange South | 9143 Phillips Highway Suite 500, Jacksonville, FL 32256 | Preschool |
| Eagle Academy | 8985 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Early Learning Center of Arlington | 930 Cesery Boulevard, Jacksonville, FL 32211 | Center |
| Episcopal Children's Services Early Learning Center | 1070 West 18th Street, Jacksonville, FL 32209 | Preschool |
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| Exclusively Infants | 11900-225 Atlantic Blvd, Jacksonville, FL 32225 | Center |
| Faith Academy & Daycare | 6561 Firestone Road, Jacksonville, FL 32244 | Center |
| Fields Christian Academy | 6620 Arlington Expressway, Jacksonville, FL 32211 | Center |
| First Baptist Junior Academy | 125 West Ashley St, Jacksonville, FL 32202 | Preschool |
| First Christian Preschool | 11924 San Jose Blvd, Jacksonville, FL 32223 | Preschool |
| First Coast Christian School | 7587 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| First Coast Young Stars Learning Center | 3911 North Main Street, Jacksonville, FL 32206 | Preschool |
| Flower Garden Early Education | 3602 Park Street, Jacksonville, FL 32205 | Center |
| For Your Child Only # 1 | 2183 W 15th Street, Jacksonville, FL 32209 | Center |
| For Your Child Only Day Care #4 | 2135 Kings Road, Jacksonville, FL 32209 | Preschool |
| Fort Caroline Learning Tree | 7440 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Fruit Cove Baptist Weekday Preschool | 501 State Road 13, Saint Johns, FL 32259 | Center |
| Ft. Caroline Christian Pre-K | 8510 Ft. Caroline Rd., Jacksonville, FL 32277 | Preschool |
| Future Duck Early Learning & Preschool | 504 Golfair Boulevard, Jacksonville, FL 32206 | Preschool |
| Future Duck Early Learning & Preschool | 619 Golfair Boulevard, Jacksonville, FL 32206 | Preschool |
| G E B Momma's House Academy Private School | 5431 Lenox Avenue, Jacksonville, FL 32205 | Center |
| Gateway Community Services | 555 Stockton Street, Jacksonville, FL 32204 | Center |
| Gateway Nursery and Kindergarten | 641 West 4th Street, Jacksonville, FL 32209 | Preschool |
| Gingerbread House Learning Center | 6005 Chester Ave., Jacksonville, FL 32217 | Preschool |
| Good Shepherd Lutheran Church Precious Lambs Preschool | 6551 Argyle Forest Boulevard, Jacksonville, FL 32244 | Preschool |
| Grace Episcopal Dayschool | 156 Kingsley Avenue, Orange Park, FL 32073 | Center |
| Graham Bears Childcare | 5151 Colonial Ave., Jacksonville, FL 32210 | Preschool |
| Grove Park Elementary School Preschool | 1643 Miller Street, Orange Park, FL 32073 | Preschool |
| Growing Minds Development Center | 1181 Cassat Avenue, Jacksonville, FL 32205 | Center |
| Happy Acres Ranch | 7117 Crane Avenue, Jacksonville, FL 32216 | Preschool |
| Happy Hands Christian Child Care | 2017 Lane Avenue South, Jacksonville, FL 32210 | Preschool |
| Harvest Christian Academy | 1057 Arlington Road, Jacksonville, FL 32211 | Center |
| Head Start at Anders Park | 10770 Anders Blvd #407, Jacksonville, FL 32246 | Preschool |
| Head Start at Brentwood | 3465 Village Center Drive South, Jacksonville, FL 32206 | Preschool |
| Head Start at Fairfield | 515 Victoria Street, Jacksonville, FL 32202 | Preschool |
| Head Start at Ft. Caroline Road | 5543 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Head Start at Happyland Center | 1577 W 26th Street, Jacksonville, FL 32209 | Preschool |
| Head Start at Jacksonville Urban League 8th St | 20 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Head Start at Lee Road | 1210 Lee Road, Jacksonville, FL 32225 | Preschool |
| Head Start at Little Angels In Paradise | 3252 N Canal Street, Jacksonville, FL 32209 | Preschool |
| Head Start at Northeast Springfield | 1221 E 16th Street, Jacksonville, FL 32206 | Preschool |
| Head Start at Old Stanton | 521 West Ashley Street, Jacksonville, FL 32202 | Preschool |
| Head Start at Pearson | 1314 West Union Street, Jacksonville, FL 32209 | Preschool |
| Head Start at R L Wilson | 2131 Woodside Drive, Jacksonville, FL 32209 | Preschool |
| Head Start at Robert F Kennedy | 1133 Ionia St, Jacksonville, FL 32206 | Preschool |
| Head Start at The Bridge | 320 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Headstart at Shands | 2052 Jefferson Street, Jacksonville, FL 32209 | Preschool |
| Headstart at Southwind Villas | 8711 Newton Road, Jacksonville, FL 32216 | Preschool |
| Heaven Sent Daycare | 2151 Lane Ave. Suite. 102 & ..., Jacksonville, FL 32210 | Center |
| Hendricks Day School | 1824 Dean Road, Jacksonville, FL 32216 | Preschool |
| Hidden Treasures Child Care Center | 1941 Dean Road, Jacksonville, FL 32216 | Preschool |
| Highlands Precious Gifts Preschool | 10900 Mccormick Road, Jacksonville, FL 32225 | Preschool |
| Hodges Boulevard Presbyterian Church | 4140 Hodges Boulevard, Jacksonville, FL 32224 | Preschool |
| Hogan Christian Preschool | 8045 Hogan Road, Jacksonville, FL 32216 | Preschool |
| Holy Family | 9800-3 Baymeadows Road, Jacksonville, FL 32256 | Preschool |
| Honey Child Pre-School | 2627 Spring Glen Road, Jacksonville, FL 32207 | Center |
| Hope Haven Children's Clinic and Family Center | 4600 Beach Blvd, Jacksonville, FL 32207 | Preschool |
| Hopes-n-Dreams Child Care | 6689 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Hussett's Little Steppers Learning Center | 3304 Lenox Avenue, Jacksonville, FL 32254 | Preschool |
| Hyde Park Elementary Preschool | 5300 Park Street, Jacksonville, FL 32205 | Preschool |
| ISIS LITTLE HOMESCHOOL LLC | 8500 BLACKBERRY LANE EAST, Jacksonville, FL 32244 | Preschool |
| Island View Baptist Child Care Center | 900 Park Avenue, Orange Park, FL 32073 | Preschool |
| It's A Small World | 6723 Golfview Street, Jacksonville, FL 32210 | Preschool |
| It's Your World Learning Center | 2952 Edison Avenue, Jacksonville, FL 32254 | Preschool |
| Jacksonville Adventist Academy | 4298 Livingston Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Jewish Center | 3662 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Jacksonville Urban League Early Headstart | 1626 Grunthal Street, Jacksonville, FL 32209 | Center |
| Jacksonville Urban League Head Start | 3811 St Augustine Road, Jacksonville, FL 32207 | Preschool |
| Jewish Community Alliance | 8505 San Jose Boulevard, Jacksonville, FL 32217 | Center |
| John Love Elementary Preschool | 1531 Winthrop Street, Jacksonville, FL 32206 | Preschool |
| Joshua Christian Academy | 924 St. Clair St., Jacksonville, FL 32254 | Preschool |
| Juliette's Day Care | 6939 Como Road, Jacksonville, FL 32210 | Preschool |
| Julington Creek KinderCare - CLOSED | 200 N Ridgecrest Ln, Saint Johns, FL 32259 | Preschool |
| Just 4 Kids Learning Academy | 4371 Losco Rd., Jacksonville, FL 32257 | Preschool |
| Just Kids Learning Center | 120 Parkwood Drive East, Orange Park, FL 32073 | Preschool |
| Justina Road Elementary Preschool | 3101 Justina Road, Jacksonville, FL 32277 | Preschool |
| Kampus Kids | 2300 Kingsley Avenue, Orange Park, FL 32073 | Preschool |
| Kats Little Kittens Learning Center | 3692 Beach Blvd., Jacksonville, FL 32207 | Preschool |
| Kias Little Angels | 5870-2&3 San Juan Boulevard, Jacksonville, FL 32210 | Preschool |
| Kid Kampus U S A | 1868 Parental Home Road, Jacksonville, FL 32216 | Preschool |
| Kid's Academy USA | 1842 Blanding Blvd Ste 3, Jacksonville, FL 32210 | Preschool |
| Kid'z on Top II Child Care & Learning Center | 5571 Playa Way, Jacksonville, FL 32211 | Preschool |
| Kiddieville Learning Center | 6820 Daughtry Blvd. South, Jacksonville, FL 32210 | Center |
| Kids By The Hour | 13947 Beach Boulevard Suite 210, Jacksonville, FL 32224 | Preschool |
| Kids Expanding the Kingdom Christian Preschool | 3749 Crownpoint Road, Jacksonville, FL 32257 | Preschool |
| Kids Konnection | 2221 Noble Street, Jacksonville, AL 36265 | Center |
| Kids Park | 4274 Hershel St., Jacksonville, FL 32210 | Preschool |
| Kids United Childcare & Learning Center | 7037-1 Phillips Highway Suite 1, Jacksonville, FL 32216 | Preschool |
| Kids World Academy Westside Jacksonville | 4932 Blanding Blvd, Jacksonville FL, Jacksonville, FL 32210 | Center |
| Kids World of Florida | 6150 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Kids World of Florida | 2597 Oak Street, Jacksonville, FL 32204 | Preschool |
| Kids World of Florida | 4040 Confederate Point Road, Jacksonville, FL 32210 | Preschool |
| Kids in Dynamic Settings | 3271 Tiger Hole Road, Jacksonville, FL 32216 | Center |
| Kidz Klub Developmental Learning Center | 4804 Blanding Boulevard, Jacksonville, FL 32210 | Preschool |
| Kidz Quest Academy | 1820 University Boulevard North, Jacksonville, FL 32211 | Preschool |