| Kidz n Kidz Academy | 6229 Merrill Road, Jacksonville, FL 32277 | Preschool |
| Kimbari Learning Academy | 3651 Crown Point Road, Jacksonville, FL 32257 | Preschool |
| Kinder Kollege Learning Center | 1403 Myrtle Avenue North, Jacksonville, FL 32209 | Center |
| Kingdom Kare Learning Center | 5827 Seaboard Avenue, Jacksonville, FL 32244 | Center |
| Kingdom Kids at CrossRoad | 10005 Gate Parkway North, Jacksonville, FL 32246 | Preschool |
| Kings and Queens Child Care and Learning Center | 3325 Plateau Street, Jacksonville, FL 32206 | Center |
| La Core Christian Academy | 122 Arlington Road, Jacksonville, FL 32211 | Preschool |
| La Escuelita Pre School | 5415 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Lakewood Presbyterian Church Preschool | 2001 University Boulevard West, Jacksonville, FL 32217 | Preschool |
| Lakewood United Methodist Preschool | 6133 San Jose Boulevard, Jacksonville, FL 32217 | Preschool |
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| Lane Avenue Child Development Center | 1650 Lane Avenue, Jacksonville, FL 32210 | Preschool |
| Lang's Little Lambs Preschool | 5310 Lenox Ave Unit #26, Jacksonville, FL 32205 | Preschool |
| Learning Ladders Child Development Center | 11270 San Jose Boulevard, Jacksonville, FL 32223 | Preschool |
| Leslie's Angels | 2114 Tilden Street, Jacksonville, FL 32206 | Center |
| Lil' Nuggets Daycare Center | 7054 Wilson Boulevard, Jacksonville, FL 32210 | Preschool |
| Lil' Sprouts Learning Center | 8840-7 Atlantic Blvd., Jacksonville, FL 32211 | Preschool |
| Little Britches | 10829 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Little Diamonds Big Heart Day Care | 1441 East 31st Street, Jacksonville, FL 32206 | Center |
| Little Friends | 4001 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Little People Child Care Center II | 5868 Lenox Avenue, Jacksonville, FL 32205 | Preschool |
| Little Royalty Learning Center | 4833 Plymouth Street, Jacksonville, FL 32205 | Center |
| Little Saints Child Care Center | 2179 Emerson Street, Jacksonville, FL 32207 | Preschool |
| Little Sarephims Daycare | 220 Mill Creek Road, Jacksonville, FL 32211 | Center |
| Little Temples Child Care Center II | 9100 Merrill Road Unit 15, Jacksonville, FL 32225 | Preschool |
| Little Tots Day Care | 6019 Lynnwood Avenue, Jacksonville, FL 32210 | Preschool |
| Little Wise Kids Early Learning Center | 6318 Arlington Road, Jacksonville, FL 32211 | Center |
| Little Wise Kids Early Learning Center II | 5703 Arlington Road, Jacksonville, FL 32211 | Center |
| Lone Star Learning Center | 8142 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Long Branch Elementary Preschool | 3723 Franklin Street, Jacksonville, FL 32206 | Preschool |
| Magellan Academy - CLOSED | 14985 Old St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| Magellan Christian Academies | 10550 - 704 Deerwood Park Boul ..., Jacksonville, FL 32256 | Preschool |
| Mandarin Childhood Learning Center | 4228 Hood Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Prep School | 3968 Sunbeam Road, Jacksonville, FL 32257 | Preschool |
| Mandarin Presbyterian Preschool | 11844 Mandarin Rd., Jacksonville, FL 32223 | Preschool |
| McCrary's Learning Center | 6620 Arlington Expressway, Jacksonville, FL 32211 | Preschool |
| Merry Pats Preschool | 1461 Fruit Cove Road South, Saint Johns, FL 32259 | Preschool |
| Merry Pop-Ins Child Care Center | 3935 St. Johns Avenue, Jacksonville, FL 32205 | Preschool |
| Mine Yours & Ours Child Care Learning Center | 7645 Merrill Road #107, Jacksonville, FL 32277 | Preschool |
| Murray Hill Baptist Child Care | 4300 Post Street, Jacksonville, FL 32205 | Preschool |
| Nakesa's Learning Center | 727 N Mcduff Avenue, Jacksonville, FL 32254 | Preschool |
| Naval Air Station Jacksonville Child Development Center | 705 Child Street, Jacksonville, FL 32212 | Preschool |
| New Dimension Christian Early Education Center | 5505 Normandy Bvld, Jacksonville, FL 32205 | Preschool |
| New Dimensions Learning Center | 3612 Belfort Road, Jacksonville, FL 32216 | Preschool |
| Northern Lights Sunshine Academy | 6240 Fort Caroline Road #1-4, Jacksonville, FL 32277 | Preschool |
| Oak Hill Elementary Preschool | 6910 Daughtry Blvd South, Jacksonville, FL 32210 | Preschool |
| Oakleaf Village Elementary Preschool | 410 Oakleaf Village Parkway, Orange Park, FL 32073 | Preschool |
| On The Right Track Child Development Center | 3325 Plymouth Street 14, Jacksonville, FL 32205 | Preschool |
| Once Upon A Time Development Center | 1129 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Open Arms Christian Childcare Center | 9039 Beach Boulevard, Jacksonville, FL 32216 | Preschool |
| Orange Park Presbyterian Church Preschool | 1905 Park Avenue, Orange Park, FL 32073 | Preschool |
| Orange Park's Little Miracles | 1981 Wells Rd, Orange Park, FL 32073 | Preschool |
| Ortega Hills Preschool | 5029 Greenway Drive, Jacksonville, FL 32244 | Preschool |
| Ortega Play School | 4807 Roosevelt Boulevard, Jacksonville, FL 32210 | Preschool |
| Page of Learning | 5809 Southpoint Parkway, Jacksonville, FL 32216 | Preschool |
| Parkwood Weekday Early Education Center | 7900 Lone Star Road, Jacksonville, FL 32211 | Preschool |
| Parson's Little Scholars Creative Child Care | 6505 Fort Caroline Road, Jacksonville, FL 32277 | Preschool |
| Parsons Christian Academy | 5705 Ft. Caroline Road, Jacksonville, FL 32277 | Preschool |
| Peas In A Pod Learning Center | 9652 Hood Road, Jacksonville, FL 32257 | Preschool |
| Pinedale Elementary Preschool | 4229 Edison Avenue, Jacksonville, FL 32254 | Preschool |
| Plantation Christian Academy | 6263 Roosevelt Boulevard, Jacksonville, FL 32244 | Preschool |
| Pokey's Angelic Christian Children's Academy | 3863 St. Augustine Road, Jacksonville, FL 32207 | Preschool |
| Pooh + Friends Child Care Development Center | 6029 Bennett Road, Jacksonville, FL 32216 | Preschool |
| Power Challengers Ministries | 4316 Barnes Road, Jacksonville, FL 32207 | Center |
| Praise Is What We Do Early Learning | 1225 West 29th Street, Jacksonville, FL 32209 | Preschool |
| Precious Angels Child Care & Learning Center | 1144 Arlington Road, Jacksonville, FL 32211 | Preschool |
| Precious Promises Preschool | 10365 Old Saint Augustine Road, Jacksonville, FL 32257 | Preschool |
| Primrose School of Julington Creek | 480 State Road 13 North, Saint Johns, FL 32259 | Preschool |
| Progressive Learning Center | 1855 Hamilton Street, Jacksonville, FL 32210 | Preschool |
| Promise Land Preschool | 3990 Loretto Road, Jacksonville, FL 32223 | Center |
| Punkin Place Child Development of Jax | 6641 San Juan Avenue, Jacksonville, FL 32210 | Preschool |
| Resurrection Catholic School | 3406 Justina Road, Jacksonville, FL 32277 | Preschool |
| Rising Stars of Tomorrow Preschool | 6304 Hyde Park Haven, Jacksonville, FL 32210 | Preschool |
| River of Life Child Care Center | 4537 Emerson Street, Jacksonville, FL 32207 | Center |
| Ronelli's Academy | 12078 Old St. Augustine Road, Jacksonville, FL 32258 | Center |
| Ruth Upson Elementary Preschool | 1090 Dancy Street, Jacksonville, FL 32205 | Preschool |
| S. P. Livingston Elementary Preschool | 1128 Barber Street, Jacksonville, FL 32209 | Preschool |
| Sacred Heart Catholic School | 5752 Blanding Boulevard, Jacksonville, FL 32244 | Preschool |
| Saint Joseph Catholic School | 11600 Old St. Augustine Road, Jacksonville, FL 32258 | Preschool |
| Saint Marks Ark Child Development Center | 3976 Hendricks Ave, Jacksonville, FL 32207 | Preschool |
| Saint Paul's Catholic School | 2609 Park Street, Jacksonville, FL 32204 | Preschool |
| Saint Pius School | 1470 W. 13th St., Jacksonville, FL 32209 | Preschool |
| San Carlos Learning Center | 2045 Gilmore Street, Jacksonville, FL 32204 | Preschool |
| San Jose Catholic School | 3619 Toledo Road, Jacksonville, FL 32217 | Preschool |
| San Jose Elementary Preschool | 5805 St. Agustine Road, Jacksonville, FL 32207 | Preschool |
| Seacoast Christian Academy Preschool | 9570 Regency Square Boulevard, Jacksonville, FL 32225 | Preschool |
| Sensational Kids Learning Academy | 6043 Terry Road, Jacksonville, FL 32216 | Preschool |
| Seventh Generation Academy | 10679 Old St. Augustine Rd, Jacksonville, FL 32257 | Preschool |
| Sharing Tree Preschool | 6045 Greenland Road, Jacksonville, FL 32258 | Preschool |
| Shepherd of the Woods Lutheran School | 7860 Southside Blvd, Jacksonville, FL 32256 | Preschool |
| Shepherd of the Woods Lutheran School II | 6595 Columbia Park St., Jacksonville, FL 32258 | Preschool |
| Smart Start Learning Center | 1112 Fountain Road, Jacksonville, FL 32205 | Preschool |
| Smitty's Child Care and Pre-School | 7710 Hilsdale Road, Jacksonville, FL 32216 | Preschool |
| Soaring High Learning Center | 4218 Blanding Bvd, Jacksonville, FL 32210 | Preschool |
| South Jacksonville Presbyterian Preschool | 2137 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Southside United Methodist Church Preschool | 3120 Hendricks Avenue, Jacksonville, FL 32207 | Preschool |
| Spring Park Elementary Preschool | 2250 Spring Park Road, Jacksonville, FL 32207 | Preschool |
| St Catherine's Episcopal Preschool | 4758 Shelby Avenue, Jacksonville, FL 32210 | Preschool |
| St Stephen Child Care & Learning Center | 1525 North Davis Street, Jacksonville, FL 32209 | Preschool |
| St. John's Presbyterian Kindergarten | 4275 Herschel Street, Jacksonville, FL 32210 | Center |
| St. Johns Cathedral School and Early Learning Center | 330 North Market Street, Jacksonville, FL 32202 | Preschool |