| Johnson City KinderCare | 906 Sunset Dr, Johnson City, TN 37604 | Preschool |
| 21st Century Homework Club | 907 Kingsprings Road, Johnson City, TN 37601 | Center |
| Ashley Academy | 1502 Knob Creek Road, Johnson City, TN 37604 | Center |
| Babyzone Child Care Center | 103 L.p. Auer Rd., Johnson City, TN 37604 | Center |
| Boone Trail Early Learning Center | 1985 Carroll Creek Rd., Johnson City, TN 37615 | Preschool |
| Boones Creek Child Development Center | 1806 Old Gray Station Rd, Johnson City, TN 37615 | Center |
| Boones Creek Elementary - SACC | 348 Boones Creek, Johnson City, TN 37615 | Center |
| Boones Creek Head Start | 348 Christian Church Rd, Johnson City, TN 37615 | Preschool |
| Carver Head Start | 500 Washington St, Johnson City, TN 37601 | Center |
| Central Baptist Preschool | 300 North Roan Street, Johnson City, TN 37601 | Preschool |
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| Central Elementary - Head Start | 252 Taylor Town Rd., Johnson City, TN 37601 | Preschool |
| Central Elementary Carter Cares | 252 Taylortown Road, Johnson City, TN 37601 | Center |
| Cherokee Christian Learning Center | 2615 South Roan Street, Johnson City, TN 37601 | Preschool |
| Children First Head Start Center | 837 Pardee St., Johnson City, TN 37601 | Preschool |
| Children's Center Of Sycamore Shoals Hospital | 408 Bemberg Road, Elizabethton, TN 37643 | Center |
| Coalition For Kid's - Kid's In Action | 2308 Watauga Rd., Johnson City, TN 37601 | Center |
| Coalition For Kids Mountain View Elementary | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Coalition For Kids North Side Elementary | 1000 N. Roan St., Johnson City, TN 37601 | Center |
| Community Day Care & Learning Center | 820 Hemlock St., Elizabethton, TN 37643 | Center |
| Community Day Care & Learning Center | 210 South Hills Dr, Elizabethton, TN 37643 | Center |
| Crystal's Cuties | 1497 Southside Rd., Elizabethton, TN 37643 | Center |
| Early Learning Center - First United Methodist Church | 900 Spring Street, Johnson City, TN 37604 | Center |
| East Side Day Care & Learning Center | 206 Old Siam Road, Elizabethton, TN 37643 | Center |
| East Side Elementary Pre-kindergarten | 800 Siam Road, Elizabethton, TN 37643 | Preschool |
| Elizabethton Boys And Girls Club | 104 Hudson Drive, Elizabethton, TN 37643 | Center |
| Etsu Child Study Center | 2101 Signal Drive, Johnson City, TN 37604 | Center |
| Fairmont Elementary - Educare | 1405 Lester Harris Road, Johnson City, TN 37601 | Center |
| First Christian Church Preschool | 200 Mountcastle Drive, Johnson City, TN 37601 | Preschool |
| First Presbyterian Church Preschool | 105 South Boone Street, Johnson City, TN 37604 | Preschool |
| First United Methodist Preschool | 325 East E Street, Elizabethton, TN 37643 | Preschool |
| Gray Elementary - SACC | 755 Gray Station Road, Johnson City, TN 37615 | Center |
| Gray Elementary Pre-kindergarten | 755 Gray Station Road, Johnson City, TN 37615 | Center |
| Gray United Methodist Church Preschool | 2108 Oak Street, Johnson City, TN 37615 | Preschool |
| Hampton Elementary Carter Cares After | 408 Highway 321, Hampton, TN 37658 | Center |
| Hampton Elementary Head Start | 408 Highway 321, Hampton, TN 37658 | Preschool |
| Hampton Elementary Head Start II | 408 Highway 321, Hampton, TN 37658 | Preschool |
| Happy Valley Elementary Head Start | 1840 Milligan Highway, Johnson City, TN 37601 | Preschool |
| Happy Valley Elementary School - Extended School Program | 1840 Milligan Highway, Johnson City, TN 37601 | Center |
| Happy Valley Middle School Leaps | 163 Warpath Lane, Elizabethton, TN 37643 | Center |
| Harold Mccormick - Extended School Program | 226 Cedar Avenue, Elizabethton, TN 37643 | Center |
| Hospitots Child Care Center | 1719 West Market Street, Johnson City, TN 37604 | Center |
| Indian Trail Intermediate School Educare | 307 Car-mol Drive, Johnson City, TN 37601 | Center |
| Jack And Jill | 1620 Old Boones Creek Rd, Johnson City, TN 37615 | Center |
| Johnson City Kidz Clubhouse | 408 West Market St., Johnson City, TN 37604 | Center |
| Johnson City Prek At Fairmont Elementary | 1405 Lester Harris Road, Johnson City, TN 37601 | Preschool |
| Jonesborough Elementary - SACC | 306 Forest Drive, Jonesborough, TN 37659 | Center |
| Jonesborough Middle School SACC | 308 Forest Drive, Jonesborough, TN 37659 | Center |
| Keenburg Carter Cares | 139 Keenburg Road, Elizabethton, TN 37643 | Center |
| Keenburg Elementary Head Start | 139 Keenburg Road, Elizabethton, TN 37643 | Preschool |
| Kidz Academy | 5452 Highway 11e, Piney Flats, TN 37686 | Center |
| Lake Ridge Elementary - Educare | 1001 Lake Ridge Square, Johnson City, TN 37601 | Center |
| Lake Ridge Elementary - Preschool | 1001 Lake Ridge Square, Johnson City, TN 37601 | Preschool |
| Lake Terrace Head Start | 2450 Mint Hill Rd, Johnson City, TN 37601 | Preschool |
| Lamar Elementary - SACC | 3261 Highway 81s, Jonesborough, TN 37659 | Center |
| Lamar Elementary Pre-kindergarten | 3261 Highway 81 South, Jonesborough, TN 37659 | Preschool |
| Leaps Jump In - Jump Ahead | 820 West Market Street, Johnson City, TN 37604 | Center |
| Learn And Grow Childcare Center | 604 Boones Creek Road, Jonesborough, TN 37659 | Center |
| Little Bo Peep Learning Center | 305 West Main, Jonesborough, TN 37659 | Center |
| Little Buccaneers Student Child Care Center | 807 University Parkway, Johnson City, TN 37614 | Center |
| Little Lambs Learning Center And Day Care | 1207 Broad St., Elizabethton, TN 37643 | Preschool |
| Little Tigers Preschool & Enrichment | 305 Forest Dr, Jonesborough, TN 37659 | Preschool |
| Mother Goose Day Care Center | 709 N. Boone St., Johnson City, TN 37604 | Center |
| Mountain View Baptist Daycare & Learning | 4005 Bristol Hwy., Johnson City, TN 37602 | Center |
| Mountain View Elementary Educare | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Munsey Memorial Preschool | 201 E. Market St., Johnson City, TN 37601 | Preschool |
| North Side Elementary School Educare | 1000 North Roan Street, Johnson City, TN 37601 | Center |
| Northside Elementary Pre-kindergarten | 1000 N. Roan, Johnson City, TN 37601 | Preschool |
| Parkway Preschool | 116 Claude Simmons Road, Johnson City, TN 37604 | Preschool |
| Persimmon Ridge Head Start | 1521 Persimmon Ridge Rd, Jonesborough, TN 37659 | Preschool |
| Pinecrest Head Start | 500 Angel Drive, Johnson City, TN 37601 | Preschool |
| Playstation - The Kids Depot | 1006 South Broadway, Johnson City, TN 37601 | Center |
| Princeton Preporatory | 504-a Princeton Road, Johnson City, TN 37601 | Center |
| Range Elementary Carter Cares | 655 Watauga Road, Watauga, TN 37694 | Center |
| Range Elementary School Pre-kindergarten | 655 Watauga Road, Watauga, TN 37694 | Preschool |
| Ridgeview Elementary Idea 619 Pre-kindergarten | 252 Sam Jenkins Road, Johnson City, TN 37615 | Preschool |
| Ridgeview School Age Child Care | 252 Sam Jenkins Road, Johnson City, TN 37615 | Center |
| Rise Up! | 1500 East Millard Street, Johnson City, TN 37601 | Center |
| Science Hill Preschool | Technology Center, Johnson City, TN 37601 | Preschool |
| Shepherd's Pasture Preschool | 1112 Cherokee Road, Johnson City, TN 37604 | Preschool |
| Shining Stars Christian Learning | 2615 South Roan Street, Johnson City, TN 37604 | Center |
| Small Steps Children's Academy | 1201 East Chilhowie Ave., Johnson City, TN 37601 | Center |
| St. Mary's School | 2211 East Lakeview Drive, Johnson City, TN 37601 | Center |
| Stepping Stones Day Care And Preschool | 216 East Market St., Johnson City, TN 37601 | Preschool |
| Sundale Preschool | 2106 Sundale Road, Johnson City, TN 37604 | Preschool |
| Telamon Early Head Start | 1207 Broad Street, Elizabethton, TN 37643 | Center |
| Telamon Head Start I | 551 Mocking Bird Court, Unicoi, TN 37692 | Preschool |
| Telamon Migrant Head Start II | 553 Mocking Bird Court, Unicoi, TN 37692 | Preschool |
| Tennessee Tots Learning Center | 440 Blue Ridge Drive, Elizabethton, TN 37643 | Center |
| Tiny Town | 1012 Knob Creek Road, Johnson City, TN 37604 | Center |
| Toddler Time Day Care Center | 435 Highway 321, Hampton, TN 37658 | Center |
| Tri-cities Christian Preschool & Aftercare - Gray | 2102 Maple Dr, Johnson City, TN 37615 | Preschool |
| Unicoi After School Enrichment Program | 404 Massachusetts Avenue, Unicoi, TN 37692 | Center |
| Unicoi Child Care Center | 4029 Unicoi Drive, Unicoi, TN 37692 | Center |
| Unicoi Elementary Pre-kindergarten | 404 Massachusetts Avenue, Unicoi, TN 37692 | Preschool |
| Valley Forge Carter Cares | 1485 Riverview Drive, Elizabethton, TN 37643 | Center |
| Valley Forge Elementary Pre-kindergarten | 1485 Riverview Drive, Elizabethton, TN 37643 | Preschool |
| Wesley Memorial United Methodist Church Preschool | 225 Princeton Road, Johnson City, TN 37601 | Preschool |